MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint ishida-2005-1 Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. rat-brain Rattus norvegicus brain brain Brain [cerebrum] far western blotting far western blotting Affinity blotting Affinity blotting fingerprinting peptide massfingerprinting fingerprinting Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation ishida-2005-2 Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation ishida-2005-4 Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation ishida-2005-5 Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. in vitro In vitro phosphatase assay phosphatase assay autoradiography autoradiography Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation ishida-2005-3 Identification of major Ca(2+)/calmodulin-dependent protein kinase phosphatase-binding proteins in brain: biochemical analysis of the interaction. rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip monoclonal western monoclonal antibody western blot monoclonal western Ishida A., Tada Y., Nimura T., Sueyoshi N., Katoh T., Takeuchi M., Fujisawa H., Taniguchi T., Kameshita I. aishida@asahikawa-med.ac.jp Arch. Biochem. Biophys. (0003-9861) 2005 partial coverage rapid curation ppm1e_rat Protein phosphatase 1E Ppm1e Camkn Ca(2+)/calmodulin-dependent protein kinase phosphatase N CaMKP-nucleus Partner of PIX 1 Partner of PIX-alpha protein protein rat Rattus norvegicus (Rat) Rat MAGCIPEEKTYRRFLELFLGEFRGPCGGGEPEPEPESEPEPEPEAELVAAEAAEASGEDPGEDAATVEAAEEGVQDQDPEPEEEAVEEEAAAEGEEEEEEEEAAAPGHSAVPPPQPQLPPLPPLPRPLSERITREEVEGESLDLCLQQLYKYNCPSFLAAALARATSDEVLQSDLSAHCIPKETDGTEGTVEIETVKLARSVFSKLHEICCNWVKDFPLRRRPQIYYETSIHAIKNMRRKMEDKHVCIPDFNMLFNLEDQEEQAYFAVFDGHGGVDAAIYASVHLHVNLVRQEMFPHDPAEALCRAFRVTDERFVQKAARESLRCGTTGVVTFIRGNMLHVAWVGDSQVMLVRKGQAVELMKPHKPDREDEKQRIEALGGCVVWFGAWRVNGSLSVSRAIGDAEHKPYICGDADSASTVLDGTEDYLILACDGFYDTVNPDEAVKVVSDHLKENNGDSSMVAHKLVASARDAGSSDNITVIVVFLRDMNKAVNVSEESDWTENSFQGGQEDGGDDKENHGECKRPWPQHQCSAPADLGYEGRVDSFTDRTSLSPGPQINVLEDPDYLDLTQIETSKPHSTQFLPPVEMIGPGAPKKAYVNELIMEESSVTPSQPERSGAGELLVSFNLGSTGQQICRMENLSPVYSGLENEQFKSPGKRASRLYHLRHHYSKRQRGFRFNPKFYSFFSAQESSRKIGISLSSLTRSGKRNKMLRSSLPWRENSWEGYSGNMAIRKRNNISCPDLPWDYKI mint Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Inactivates multifunctional CaM kinases aldoa_rat Fructose-bisphosphate aldolase A Muscle-type aldolase Aldoa protein protein rat Rattus norvegicus (Rat) Rat MPHPYPALTPEQKKELADIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSSLAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACTQKFSNEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYIKRALANSLACQGKYTPSGQSGAAASESLFISNHAY g3p_rat Glyceraldehyde-3-phosphate dehydrogenase Gapdh 38 kDa BFA-dependent ADP-ribosylation substrate BARS-38 Gapd Peptidyl-cysteine S-nitrosylase GAPDH protein protein rat Rattus norvegicus (Rat) Rat MVKVGVNGFGRIGRLVTRAAFSCDKVDIVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQERDPANIKWGDAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQAAEGPLKGILGYTEDQVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMAYMASKE kcc1a_rat Calcium/calmodulin-dependent protein kinase type 1 Camk1 CaM kinase I CaM kinase I alpha protein protein rat Rattus norvegicus (Rat) Rat MPGAVEGPRWKQAEDIRDIYDFRDVLGTGAFSEVILAEDKRTQKLVAIKCIAKKALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVKYLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVLAQKPYSKAVDCWSIGVIAYILLCGYPPFYDENDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGDTALDKNIHQSVSEQIKKNFAKSKWKQAFNATAVVRHMRKLQLGTSQEGQGQTASHGELLTPTAGGPAAGCCCRDCCVEPGSELPPAPPPSSRAMD Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By similarity). Recognizes the substrate consensus sequence [MVLIF]-x-R-x(2)-[ST]-x(3)-[MVLIF]. Phosphorylates EIF4G3/eIF4GII. In vitro phosphorylates CREB1, ATF1, CTFR, MYL9, SYN1/synapsin I and SYNII/synapsin II mint kcc4_mouse Calcium/calmodulin-dependent protein kinase type IV Camk4 CaM kinase-GR protein protein mouse Mus musculus Mouse MLKVTVPSCPSSPCSSVTASTENLVPDYWIDGSNRDPLGDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDARDAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVKKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHTSIQENHKASSDPPSTQDAKDSTDLLGKKMQEEDQEEDQVEAEASADEMRKLQSEEVEKDAGVKEEETSSMVPQDPEDELETDDPEMKRDSEEKLKSVEEEMDPMTEEEAPDAGLGVPQQDAIQPEY mint Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory aldoa-ppm1e 1230324 ppm1e_rat 1230329 enzyme enzyme neutral component neutral component aldoa_rat 1230330 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association false false false mint F1; T1 gapdh-ppm1e 1230324 ppm1e_rat 1230329 enzyme enzyme neutral component neutral component g3p_rat 1230331 enzyme target enzyme target substrate substrate neutral component neutral component physical association physical association false false false mint F1; T1;F2 gapdh-ppm1e-1 1230325 ppm1e_rat 1230329 unspecified role unspecified role neutral component neutral component g3p_rat 1230331 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false F3 mint aldoa-ppm1e-1 1230325 ppm1e_rat 1230329 unspecified role unspecified role neutral component neutral component aldoa_rat 1230330 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false F3 mint ppm1e-gapdh-1 1230326 ppm1e_rat 1230329 unspecified role unspecified role bait bait g3p_rat 1230331 unspecified role unspecified role prey prey physical association physical association false false false F5 mint gapdh-ppm1e-2 1230326 ppm1e_rat 1230329 unspecified role unspecified role prey prey g3p_rat 1230331 unspecified role unspecified role bait bait physical association physical association false false false F5 mint camk1-ppm1e 1230327 kcc1a_rat 1230332 enzyme target enzyme target substrate substrate neutral component neutral component ppm1e_rat 1230329 enzyme enzyme neutral component neutral component dephosphorylation dephosphorylation reaction dephosphorylation false false false F9 mint camk4-ppm1e 1230327 ppm1e_rat 1230329 enzyme enzyme neutral component neutral component kcc4_mouse 1230333 enzyme target enzyme target substrate substrate neutral component neutral component dephosphorylation dephosphorylation reaction dephosphorylation false false false F7 mint ppm1e-gapdh 1230328 ppm1e_rat 1230329 unspecified role unspecified role bait bait g3p_rat 1230331 unspecified role unspecified role prey prey physical association physical association false false false F4 mint