IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lai-2004b-4 The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate axon outgrowth in C. elegans. chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lai T., Garriga G. COS cells were used , but the exact cell line is not specified. garriga@berkeley.edu mixed stage C. elegans cDNAs Development (0950-1991) 2004 Only protein-protein interactions imex curation lai-2004b-1 The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate axon outgrowth in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lai T., Garriga G. garriga@berkeley.edu mixed stage C. elegans cDNAs Development (0950-1991) 2004 Only protein-protein interactions imex curation lai-2004b-2 The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate axon outgrowth in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lai T., Garriga G. garriga@berkeley.edu mixed stage C. elegans cDNAs Development (0950-1991) 2004 Only protein-protein interactions imex curation lai-2004b-3 The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate axon outgrowth in C. elegans. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lai T., Garriga G. garriga@berkeley.edu mixed stage C. elegans cDNAs Development (0950-1991) 2004 Only protein-protein interactions imex curation lai-2004b-5 The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate axon outgrowth in C. elegans. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lai T., Garriga G. wild-type UNC-51 was purified from COS cells, but the exact COS cell line used is not stated. garriga@berkeley.edu mixed stage C. elegans cDNAs Development (0950-1991) 2004 Only protein-protein interactions imex curation g5ecq1_caeel K10D3.2 unc-14 CELE_K10D3.2 protein protein caeel Caenorhabditis elegans MVELCELHMMPEEDQWPDDIDARIAALNFCGEDSMLFESVDPSVSTDSLDSQQFRERCQMKKEDFQLAFADSGHWQSGINDNLTTWGRIRTSEPLDERTASAPDVWNVKRSDSARSPNRPNSLIANFVSGDATRFVDVNDNEIREANEEIIRKDRWRRDSARRCSSGGQNQKRTFADILEKNVTAPTSMAITSSDNEKPPKLDFLAMHHEMPSLCESFTASFRDAIIKMQKCEPLPSITSTNDFPLFFQEDSPDSGLGCSGPSHIEDWQSLSVLLPKHVAEACSFFKSNTQLLTSSTSKTAPQTSTNIVSNCIDRRISGISASANEACRTCYRVRRRIHPPVWAQTAQSKTVLCDCASTPTDTNFSFAPTTSTTRHQLRAKELSIVGLPIYAAKRTLVENVVEGVAAISRGDGSDLLVIAMRCLIEDGLQENVSAWTMIQTVTSKGPATKDVHSIVKQLEECSKTDNVKVEIFFEELIRENSLDCWLCYIVLKEKVLKTLYSENAFLLSASSEYRTLLWRMVDSLSLLPVIEARSDSVHQQFKSMQQWGGASRIASDSRVPKSSSFPARLSTAPSRRSRIPLSTSRISISSTTSTPRSARSPSTTSRIRVASIMGDFTLANFSLSDGEKVSVLSTRGGLARCVRLTTSHSKINNGVIPIEHLLFQ unc51_caeel Serine/threonine-protein kinase unc-51 unc-51 Y60A3A.1 Uncoordinated protein 51 protein protein caeel Caenorhabditis elegans MEQFDGFEYSKRDLLGHGAFAIVYRGRYVDRTDVPVAIKAIAKKNISKSKNLLTKEIKILKELSSLKHENLVGLLKCTETPTHVYLVMEFCNGGDLADYLQQKTTLNEDTIQHFVVQIAHALEAINKKGIVHRDLKPQNILLCNNSRTQNPHFTDIVIKLADFGFARFLNDGVMAATLCGSPMYMAPEVIMSMQYDAKADLWSIGTILFQCLTGKAPFVAQTPPQLKAYYEKTRELRPNIPEWCSPNLRDLLLRLLKRNAKDRISFEDFFNHPFLTSPLLPSPSKRILESARSPLLANRRIITPQSSLPVPKRAGSTKLDSPTPVRRIGESPRVQRRVITPGMPSPVPGAPMQESTDFTFLPPRQESSPVKQVQVHTNVSPSLTTCKPVPVPSQRLTYQKMEERLAAARKTAVPSSSSPTGSAVSAQHQHQHQQQQEPASSPVVQRIERPDQLPRRTTLQDPNAHDIERMTMPNPTFVVCGSSTKPSPNNANRVRRSTITSPADTQDMVAADQMLSNLDPTTTTTTIPKSATTANIQGIPRGARDRSVTSPPQPTIHENEPLDNAKYQQTDVNNSPTAPTEPFIIKNQTTCSTSSTSSSVVEEEEAMSLPFASGSHLAAGFKKTPAEVPMDHGALPPALDQEIVLGEEHKQILAKLRFVAELVDTLIHVAEQKDNPLASAMASRRQLLTTGTSTTNTSSPYRRAEQLVVYVRALHMLSSALLLAQTNVANRVLHPSVAVQQVLNQLNDKYHQCLVRSQELASLGLPGQDPAMAVISAERIMYRHAIELCQAAALDELFGNPQLCSQRYQTAYMMLHTLAEQVNCDQDKTVLTRYKVAVEKRLRILERQGFVAAVNT ki26l_caeel Kinesin-like protein vab-8 vab-8 Variable abnormal morphology protein 8 K12F2.2 protein protein caeel Caenorhabditis elegans MEACSSKTSLLLHSPLRTIPKLRLCASISSEDVAHGRCSLTDQHLQIEGKNYSKTTFDHIFRTDATQDDMYTAFLSDTINSVFAGNDATVLAMGAKTNGKDERLYGNSVSRNGLVQMAITQLMNALDDNKDSEERIQVRMSAIMVSQNESSIVDLLSPFNPDPRHRVVKIVDDARTGVFIDNESEIRVETIDQALFYLNTAVDHRMIQDEHTHRTSHVFISLSLYSYKMGDKMQGGRRRLCFLDMGIGERNSTNGGMTMPALGSILLAMVQRNKHIPSRDSSVCQLIRCALSTSRFTTFVFSFGAKSDDNENIAHLACKIARTRAKSMVGHGRKSSGTMSTGTMESNSSSCGTTTITPGGTPRTQRRFELESGSELSAAETVIFLGPNSSRTASPASTTMPFTPTSIRPLHRTTRNHSGVEALSKPLSVETKSSPTHNCHDGCIHSIPPMLRGHTPFLSASLKLYDELCSPPSSSRASPAPPAAFGGKTSEKREDFGIMIAQPSIPLMKAKSKYNLDDGKMKQIMQWMETSEAPPILFSSPCYENSATSVEELRECVGILSHPLEDIIEQEEESMRTSTATTGGSKKDHPLRILSKQDLNVEPEIKDKQEENELELVMAASLSSMRSHDILAKLEAMRNAQNGNSVQNIGNSQSNTDMDVSEMDVYRRASHLEEYAMQRVREIEENKLKNKKKIKLGLNCCQQQSMISSGSTVVDWSQIERKKEKEREVHEEEMRKEMLRERRAKLKITELEIKRERNLIDKELDDKKGIANSIARQLQHFSLSPCRGGRTHRSVSTHRIDPPNASLPSTPTMSHKKVIGGSLAKLSASGASGSTGAPPSPALGYHQSLPRHSKLPTSVNGRRASAERERKSNKASRNSCSKERKISGSKEELQWRSPYAQMTSPKSYGGPGTSSSGRGSSAPGSDFETPVVSTTEKSANGTIPRSKRQSYSASSGYESANDYHIYSTTNKKPHILDKKRNEEKLSLVRQADEIRHRQWQLKKELEEAKRAIGQEDDAKMIANSSDQRLNGLSRTTMIDAMLQENRILEKRLVACRNHSMLVTTFI unc_51-unc_14-2 1144316 n/a 1144321 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1144322 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7A/B unc_51-vab_8-1 1144316 n/a 1144323 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 1144322 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig 7D vab_8-unc_51 1144317 n/a 1144323 unspecified role unspecified role bait bait n/a 1144322 unspecified role unspecified role prey prey physical association physical association false false false vab_8-unc_51-1 1144318 n/a 1144322 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1144323 unspecified role unspecified role bait bait physical association physical association false false false Fig 1A unc_51-vab_8 1144319 n/a 1144322 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 1144323 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig 1B/C unc_14-unc_51-1 1144320 n/a 1144321 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 1144322 enzyme enzyme neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false caere Caenorhabditis remanei Caenorhabditis vulgaris phosphorylation phosphorylation reaction phosphorylation false false false Fig 7C UNC-14 is phosphorylated by UNC-51