IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kitagawa-2002-1 The Cdc20 homolog, FZY-1, and its interacting protein, IFY-1, are required for proper chromosome segregation in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kitagawa R., Law E., Tang L., Rose AM. snf1-snf4 interaction from Figure S4 has not been added as snf4 is not in UniProt. WormBase reports that this is pseudogene arose@gene.nce.ubc.ca Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation kitagawa-2002-3 The Cdc20 homolog, FZY-1, and its interacting protein, IFY-1, are required for proper chromosome segregation in Caenorhabditis elegans. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kitagawa R., Law E., Tang L., Rose AM. snf1-snf4 interaction from Figure S4 has not been added as snf4 is not in UniProt. WormBase reports that this is pseudogene arose@gene.nce.ubc.ca Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation kitagawa-2002-2 The Cdc20 homolog, FZY-1, and its interacting protein, IFY-1, are required for proper chromosome segregation in Caenorhabditis elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kitagawa R., Law E., Tang L., Rose AM. snf1-snf4 interaction from Figure S4 has not been added as snf4 is not in UniProt. WormBase reports that this is pseudogene arose@gene.nce.ubc.ca Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation fzy1_caeel WD repeat-containing protein fzy-1 fzy-1 Fizzy protein 1 CDC20 protein family homolog 1 ZK177.6 protein protein caeel Caenorhabditis elegans MNNKGRTPGSAGRTVRSSAQQNGLTMRKRDMTPTRNTNLLPNATFVGDRFLGVRLDQDELDHANHLMTSKLYSNKENLNNSMSEPNSPEKKSVEGEALKQMMRHKSTGALTDADDGDRILCYKKNLAPPPAIGYINQAKVLYSTNSVINPASSVKKSTRHVKETATKVLDGPGLTKDLYSRHLDWGCHNWVAVALGHELYLWNTETCVIKNLFEDNAPTNEGLITSVRWSQEGRYISLGYASGAVKIYDPNRPKTTEYVRELRTLRVGGASRCASIAWRKQGVMTCGYKSGDIVNHDVRISQHVVSSWGGDNGHCRDVTALEWSADENMCVSGSSDRTAKIWDGRHVRGSTVIQDPEPMFTIDEHTGQVRTAQFCSFRDGILATGGGINDGTVKLWDVKRQFQKVRELNVCETGGVGGIVFNRPYSEMLTASDDGFLRIYRFNANYKLSHEIQASNEPIMDLVGSPFDEVLIGDMEETLKVFQLFNVDKSTNILDRTAPKNVGLNVR ify1_caeel Securin-like protein ify-1 Interactor of Fizzy protein C27A2.3 protein protein caeel Caenorhabditis elegans MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLASACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAADIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDFNYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEANLPL q9ngt3_caeel mdf-2 Y69A2AR.30 CELE_Y69A2AR.30 protein protein caeel Caenorhabditis elegans MTDVKTQNAISLKGSAQLVKEFFHFGLNSILYQRALYPSDSFKREKKYGLTLWVAHEKKLQAFMDPLLQQVEYWLAKRQLKRLVMVISEVKTKEVVERWQFDIHTENLAEEGENAHRVKEEKKIRQEISDVIRQITASVSFLPLLEEPVSFDVLIYTGKDTQAPEDWTESGACLIQNSETVQLRSFSTSVHSVNTNVQYKADF sep1_caeel Separin homolog sep-1 sep-1 Separase Y47G6A.12 protein protein caeel Caenorhabditis elegans MKITNKSVDKQHIEKLDELRKNVSCTVIGFAEQTAELQQEISELFIAEFGVNGPIDMNSLSKLARITSYYASSEYFQGLAKYQRTACKMFITWQTLRKEAMECRSKDREIFASIPAKLCFFYFYNGELCRAVVCLLDYIDLSDDTLAKEAALRWLMFLGETELIEKKLKTWKMDKSSKDMFSATEFAMNYLKKSEYRVEMLEKLMKLRDKVKSDPTRSFSRYELASYVSWLCSTLSNVPVGSALRECEFPDRVSHIQEAALKSDSLVRNRIPGLASSQFDNSVNASIWPFLDGHQEDSNYYVHIGSTIAWHFEMRRECALVNVTTAQTRDSMSAMILNLRVALKSASFFRVLQTTNTLAYYSSIIEEAGSEKNAKLMRVSCVNLLSSNPIIVRCSTPKETGATSRAHTPMAGSSVSEKQNTMRPDLADLLGDLELLDEQSFHPITRSCVCNVCTIYPLHSSFAAEYMMSYAIHSDFSQLSIKHFNDEFARIRERGMSSQVLMHRDSSVRPRPNIIQNEIFGMCVIRWLTKKLDSKESADEDTMEIFNNALKIVRYLQQRTTDMILAVTQLGRQLEFPMECNYSWMRPTIRKPRVKATIDCAVDILRAVSPFGRRPKVEKLEKNLQPFDKERFEKVRLAMRNEMNHYGHILYREWRCRLFAYVGRTSRDPWEAAYAWAESTQIGARNAVQSRLEKCKRGLVTMSGHDRFKTCVQSMPDEMTLVQIAMADDKTIYLVKLHADRDPIIMPLAHYSQAVELMDKFTFLLDEDEMIAKYPGDITPEEFWKRRKIVDGRMMTFVDEVQKHFLGVAASLLMPSGQLGPKAAELAIKIHKLSKGGLLLGEAKEMVYQSKLMDAKSWEALILRFCEMRTTDEKFKSFLPLMHRNSVEVMNQDDSIVTEKKYTYLVICPHLSQFCWERLPIFDEYPYVGRQVSIHSTFSQLEAMKSQEKQIPLQIDVQNAYYILDPDNNLGETQKRMVEYINKFNWEGTVGSAPKSNEISAALSQRDAFFFIGHGSGSSVMPRSVLKQSTCNAISLLMGCGSVRTIPQALGFDGKTAILDYAMAKCPLIVGCLWTVTDGEIDRFLIRMIDDCFEDSKSLTGIDKLRQLSEAMHEARSKARLKYLTGAAVVMYGLPVVAKQTTPFVEKDQRNLPQTPKTSARTSMRMETVPKTPKQEFVTSKSVPMTPIFSNNENKSPSRARMPSRVLKTPRQVKTFQEEDDEAPKRSTTRQLKPLVAPPIPATPTTRTTRSSARTPSRSRNL fzy1-ify_1 1015985 n/a 1015988 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 1015989 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false fzy1-mdf_2-1 1015985 n/a 1015990 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 1015988 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false ify_1-sep_1 1015986 n/a 1015991 unspecified role unspecified role prey prey experimental prep experimental preparation experimental prep 35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1015989 unspecified role unspecified role bait bait electroporation electroporation gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Fig. 4B ify_1-sep_1-1 1015986 n/a 1015989 unspecified role unspecified role bait bait electroporation electroporation gst his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro n/a 1015991 unspecified role unspecified role prey prey experimental prep experimental preparation experimental prep 35s 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 autoradiography autoradiography undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro physical association physical association false false false Supp. Fig. 5 sep_1-ify_1 1015987 n/a 1015989 unspecified role unspecified role prey prey n/a 1015991 unspecified role unspecified role bait bait physical association physical association false false false Fig. 4B fzy_1-mdf_2 1015987 n/a 1015990 unspecified role unspecified role unspecified role unspecified role experimental prep experimental preparation experimental prep yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast n/a 1015988 unspecified role unspecified role unspecified role unspecified role experimental prep experimental preparation experimental prep yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false Supp. Fig. 4