IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ korswagen-2002-2 The Axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Korswagen HC., Coudreuse DY., Betist MC., van de Water S., Zivkovic D., Clevers HC. rkors@niob.knaw.nl mixed stage C. elegans cDNA Genes Dev. (0890-9369) 2002 Only protein-protein interactions imex curation korswagen-2002-1 The Axin-like protein PRY-1 is a negative regulator of a canonical Wnt pathway in C. elegans. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Korswagen HC., Coudreuse DY., Betist MC., van de Water S., Zivkovic D., Clevers HC. rkors@niob.knaw.nl mixed stage C. elegans cDNA Genes Dev. (0890-9369) 2002 Only protein-protein interactions imex curation bar1_caeel Beta-catenin/armadillo-related protein 1 bar-1 pvl-1 spy-1 Protruding vulva protein 1 Suppressor of polyray 1 C54D1.6 protein protein caeel Caenorhabditis elegans MDLDPNLVINHDDTNLSEASFTMEQHTSSYSDIHMGSTPCTGHRKVDMWRNHNFDSGFQTMNHSEAPSIISSLHPSSHLSGMSSMADYEPIPTLSDQQKQKFDGITQNQADGQYNTVRAIPELTMLMKDQDNEVVHKAVILMQNIAKMECDPMRRQNEARIVDPRVIFTLRDLLRDKVEFPNIIRCTLGTFFHICNRQEGIDLVTRAIAEQPDIIPNLIRHIGTYPSSIYKYAILTMHSILSDKQRGGQSVIIARQQDAITHVTPWLEAEKSEKLLPVIVDLIRVLCEKNTEQKIKFVKMGGPQKLLMLLQHRVYENLLWRTTQLLKTFSNFDAPNLVAFGGRQILANLLSHGSPRLVQSTLETLRNISDVPSKIKEDLLLKSLLELVNSRNTTIRLYSAQIMSNLVANNRHNKEFMCGNNGVVILVRALTIATKEMGDLRDKEAQQMEDYIESLICTLRHLCVGHPMSDKVQAFVFRDPALFLHKLLTMRPVLLKHTLSLLLKVVSQHALLAPFRSCRIGDKGFVEQLIHILRVACTQLNVQESIEGVRVKDIIHLCIQILRWITRDQDILNEVVFFLQTPENSRMGDGHTLPIFVLQKANVEENTKSSALALIYNLMHHEQMANVLDRDDVLVKMLQNVQMQSQTHPELASLANNILKMMYEKREKTRNTLPRYNSYLESQFGHMSMTTPRSEALNSSGEVCEGAGEQWSTPLTDDTMMDSYCNSSGRDSSKPYNSPMYHSPPAMYPEYSIGPPETYLDPHATASCYPRPTPPQYNSYDRSPPVYNDLPSNPGPSSHSSDYYPSRNSRF pry1_caeel Axin-like protein pry-1 pry-1 Protein polyray C37A5.9 protein protein caeel Caenorhabditis elegans METHLGWARSLEAVLSDRSALDAFQEWLIEYSSPQYLDLFFAIRAYERMALEGKPEKSQLSKSIYSKFLSSRTGNCEAIPKHFRAPIGEKLRHGTELEDRVFSHCSNFVQEFLRRQHEEFVGSEEFIEAFNKMSSTTADQLPGGSAHHSSHQNTMRRSSGTTSRKSAAQIATQLTAEALLKSKHDRHSKLGETKLEKMYPPTRQPYVCNATTSHNDSAVSSTFSGDTPEAHRMHSNRLRHIRDEQARENHGTMTLPRVEKASVDGQQWDHSSESGRRNFAMEITRKLLRHIDKVKLNDEMEKRIDDIEECRYTTIDMVNGTEPNDDLGKIDEDEELDDYLKMKMTDDSQKGSTNRSPKGPAGEPNKSGEGSKNTTLSPTNRAPAQLHNTIRVPRRKDYPRDTSASLKSHRHHQIDTNRMMSQSMCAPSYSSASSSYSRDSFAPAPTTRVNFAPGSSKSSQFYDSSGIGSMAPSAFSATSSLDYKDRRQHRKAPTPKKHSKIGKNLSNLITISYLGTDKIPVVTHVPNDGPMTLAEFKRHFALPNGAHQLFFKTECEDGSAPFQLLLIKDEHHLLPVFEGRIAAELR gsk3_caeel Glycogen synthase kinase-3 gsk-3 sgg-1 Y18D10A.5 protein protein caeel Caenorhabditis elegans MNKQLLSCSLKSGKQVTMVVASVATDGVDQQVEISYYDQKVIGNGSFGVVFLAKLSTTNEMVAIKKVLQDKRFKNRELQIMRKLNHPNIVKLKYFFYSSGEKKDELYLNLILEYVPETVYRVARHYSKQRQQIPMIYVKLYMYQLLRSLAYIHSIGICHRDIKPQNLLIDPESGVLKLCDFGSAKYLVRNEPNVSYICSRYYRAPELIFGATNYTNSIDVWSAGTVMAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIQSMNPNYKEFKFPQIKAHPWNKVFRVHTPAEAIDLISKIIEYTPTSRPTPQAACQHAFFDELRNPDARLPSGRPLPTLEMDGPMGTGEVSTTSGDVAGPSA mig5_caeel Segment polarity protein dishevelled homolog mig-5 Abnormal cell migration protein 5 T05C12.6 mig-5 protein protein caeel Caenorhabditis elegans MEPPCTSDCSQIKVFYYLDDETTPYVSVIEAREGVATLGNFKNSFTKRGYKYYAKELDPDIQREVKVELTTDSDRLRKSQNGFYEIFLVSTPGYGTLPRNSGTMTRPQRTALDKRRRRSADFDATPYSDASLAPSTIVSRRAGEHLAELYTSNSEDPYQYDEHTRRTGDDSSLYEPLAARDMNKIYDDDRRRKKQKKERFRRPYVPSTISSATESSVNSGLPRILEIYLPMKNVPYLGLSVCTIDGHIFVSEIAPEGAVEKDGRVNVGDQILQVNRVSFEELSGPQAVRSLREAASSKRPITLYISKFARGAPSEYDDPLASMASETMPLDVGVWVETAVQNTEKMKALGLDPQEQTATTIDDGTLPFTSTASDDEERMLYDQRRNGIPRALIEEAERKRENEQNEKIEQLTEMIDPIIVVRSMARPDSGLAVKNRKWLKILVPMSFIGRDLVDWLVDHMADIHNRKKARIYAARLLAAGLIRHVVSKLTFTEKCYYVFGDGILGNDRNSTDTTGTSGTTMRVEATTEVTYVGSPAPHALAARVGRNIPHRLETTTLSPVAHDQTWLRRRRDCESPMTNDYASMVGESQIGMNPVGNYHVFGTKNNHRQVPAPSQVTSSSLTNGSGGLGGPPPTPLSSTMVLAASPIQSQNAVNHDFDGENSSNSRTRILRT apr1_caeel Adenomatous polyposis coli protein-related protein 1 apr-1 K04G2.8 protein protein caeel Caenorhabditis elegans MSSSSSDENETTIHRTGSNTGGSGIYSQPRAGSSKRTSNVRHDVSDVDDEEEHYARFREDTAIEVDDAITVLLSSLHFEHKRDIVPTDEDDNKLRELHEKIFALITSESDVNRKRRLKKALPASNCVREQVYYLRRKPSTPPASYYHRLNAALHTIVKESFGEEYRKVATVLGLVEALAEVLILEVHTFGINETNPGEHRNIRKLIANALTNLTYGQIHSKRRLCSYDGFIRCVVRIVIESPNITQVYAGLIRNLSWNADSGMSEALQPTVHALSIAAVHAHTHRFDVTATLSALWNLAGHSVENKRTICDTPNCLKVLASLLSPDARFTSLVDSATGILKYVSQYLANTSTHLELRSLLITRMLTLLKSASFTCVTNTLGAIANLIVKDPHMQQMIRQDMAAVQQLNVLRNSNRDDIRTAVKSVLNTLNQPCSHRYGDMSHSVGGGATGMQMLSEPQLQMQTSHHAYHGTASPRLLSLRATRASPGKYIQPQAQQQLIQTPQVDQRSSSLPRHFAVQRNGFVMAQSYNQQMDQHQQQQMIYQLQQQQQIMFQTEDQAQMEHHQQIMYLQQQQQQFHQIQQQQQMQKAQEADPVPPTDDDLDIPTSTVMGTRSNSERSLGSMNPGSVMTNWNSSLDTAANSSRALSPVSYNDIPASPTMCAQVFNLPKSTESEHHQLTSQQQNTTHYSSGSANTMTRSDGATTVPMDNIITPTYAILNPILVHEQTPNGTVPRKTSEELDSPDDVLPGPSLEEEEGDYAIIGGAAQKTDDELLTRSIQSEMPTSSSTPKMKVSPRLNGFFSPTQKTTSSPAWSHPDTSPIPKSSSHRTQPNRRQDASDADRLLMESIMSEMPKSRIISPRLAGTQQYLEPEPERRSHSKNEEADRRDAFTASHEPSDHNGIDVARGSDWSPQQQLHRMESLESQASSEDSFGLTAEEPNSSTSGAAANTMRFDDEIDASLPMDCVDDDDYDYTYDHFEDYEDEEDPDATQFDDGVDAQLTIDCSMISSGSGSSQRNETTTTSRDSKALATSTPKGSASSLPGVRQATRVSTNGKSRLPVPKTNGSLVDKNPKPIIASRRPRLPPKPTLLKDKHYPEEDSIENQTRDDTIYVNAPVVEAEQERIYMNALKQQKNIEQSPSIGNGSPIAKSAIVTPYNYQKPPFTGRNNGEMSNEKSVTPNPKQMLVTIV hmp2_caeel Beta-catenin-like protein hmp-2 hmp-2 Protein humpback-2 K05C4.6 protein protein caeel Caenorhabditis elegans MLLHSTNSYSIFTDHEVETRTSRIRSAMFPDWIPPTSAAEATNSTTSIVEMMQMPTQQLKQSVMDLLTYEGSNDMSGLSLPDLVKLMCDHDESVVARAVHRAYMLSREDPNFFNAPGFDHRSFVEALMAASKSSNVNVRRNAIGALSHMSEQRGGPLLIFRSGGLAEIIRMLYDSLESVVHYAVTTLRNLLMHVSDSRAQARALNAVEALTPHLHKTNPKLLAQVADGLYFLLIDDAPSKITFLSLLGPQILVSILREYSDHRKLIYTVVRCIRSLSVCPSNKPALISLGCLPALYVELCTAKDERSQTAILVAMRNLSDSATNEENLTQLIIKLLEIIRVANDGMTACACGTLSNLTCNNTRNKQTVCSHGGIDALVTAIRRLPEVEEVTEPALCALRHCTARHSLAEEAQSELRFCQAFPVILDQLETLRTPVIKAALGVIRNSALLQTNLIELTQEQTANGHTAVSLTMDILRRAITAIEENPDIAVDGVPMWGVIEGAVSALHQLANHPAVAAACCDDIGQVGNPECPPFLDLLHRLLAHPRLGSMDDEVLEREILGLLYQLSKRPDGARAVESTGVSALLMESRGSQYKSVVTYANGVLSNLKRGDSAAIMNMSNSYDYEMSGSAADWQRDGLERELFAEMYPTNDGGHSESINMALNNSQMRPNHNWYDTDL hmr1_caeel Cadherin-related hmr-1 hmr-1 Protein Hammerhead W02B9.1 protein protein caeel Caenorhabditis elegans MSWNILLILLISNLDEVLAKTLLKLPSNAPPGWLISDLQFQNLIGDSEIATLQPSIFSTNFEVEDGYRIITNTTVTQFHGELFELFLNVKEQNFQRLVTLHVYVDPRGTSQQPATFLSTVYHATVYTSQQPGSTVVFSKPITVRNRKNFVISPISKIDKISKYSSPFSVMTRGKSVDIVMMKQKLEEDDITRHVIFLGAFTEKTGEMIAQTKVIIDVIDSGDVHFLLKSKKSIAKFASAIPANSTVFDVEKRNLSEPLLFHLEEPSRFFKIDQFSGRVSTVLPVGYGTYHIHVVARNQKKQRSDAWLEISVKKEQKLEPMTSSRSRRHLDDIVFRIPENTTMEDIEKKDMKIPLFAGETIGEINVAKEWLKIDDDGKIHLLKPLNYEKTSSIIATVPINGLQSTRTQTIRIHVADIDEPPSFVNSPLPMLAVVPLNPTIGRIVYQFVARDEHGDGDSNVLYKTIDVIPAGSFIVDPKSGVVRTGWSKYERGDTYRISAQAMDLSPSDNTTSQLSEVAILEILADERPPQFAKQEYEVTVSEDNLVDYSVVDVKAQSFRSFEDGRSKGPITYSLEGDTPEDETKWFRIDPSTGIIHLTRLLDFDDPALPKLHKLKVTAREDNRESHVDLTIRIDDVNDNVPTFTRPLYTAQVREDIPLNQTILKVTAVDKDTGDNSRITYSVDNHNFSINSNGEISAKVRLDADQLNERHFVYRFNVTARDHGEPVSLSSSAMIHIRTENTNDESAVFLPTSQYTAFVAEDAQGGTPVIQIQARDADRDEVTYSFMDKNGRSTQKMNLFSIDEHTGLVKLRHGVSAADLAEAENPINLTVIVQDDGSCCVYPSKTHTSYATLLIGIEDVNNNKPEFPDCAKYSDIAKIMEGTYKTDPPTIVKVEATDDDSSANGDIVYSLYYTQSESRKAFVIDRQTGVLTPSPHVVFDRETRPREDVTVKATDRGDRPLIGFCQFSVEVVDINDNSPQFERPSYETSVSRFEAVGTSVITVFAFDNDAAHNAEITYSLEIDTTAGEEHQNDLDFFELVNRRSGEITLIKPIPMKTQKFIFNVIADDNGIPEALQSSAQVTLNVLDKQQKAPKWQTSPDCKPGITVDENVELNKVILRCRAVSSGDSRNSDVIYKLTASGGPGNKAESKFRQFNKFENGNEWVEVVIMEGLDYEQVNNYTLTLTATDMTSRVASTKTFVVEVRDVNDVVPQFTVDLFTGTIDEEMTPNEHLEKTNGKPIVTVKAIDTDSDGPQNEVHYRIVGEANGEETKHFRIDELTGEIFPNEKFDREKIDMYILTVEASDRSVSALPGANGPNKDNVKVQIVINDVNDNAPSFEEQKYIGRVKESEGEGHDVITIKAHDLDKHSNLRYHLIGAGGGRIPFGVRTDSGTIFVKEPLDFEASDQYHLVLIASDGRHNATTNVYIHIEDVNDNAPQFEQQKYATTVIEEDVDIPKVLFNVHATDADQDEKSSRIVYRLEGQGADEVFRIGKYSGTIELVKALDRDPPAGVPSWNFVVQAIDDDGNGLVGYADVQVNVRDINDNSPIFPERLFGYIEENREPIHSDGVYFMDVQARDFDDPTTENANIEYGIVRNKLINGESVFRIDQNTGKIFAMRSLDREISSEREFIIEVRANDRGVPSREGFANVTIKVTDMNDNAPFFEKTRYEGSVEETAPIGAAVMSFSAFDADEEAKDNVFTYQLSEESDYFYVTTDKDSKQSSVGVLRVKQPLDYEDVTQRDGFHLGIRVSDGRHDAEAAVHVALVDRNDHAPHIHGATEHRVREDVPRGTSIGRYTATDRDAGDTARFRINRQSDPKRQFTIDQDGTLRVAHTLDREDIAVYNLIIEAYDNSNNIGRQMVAVYLQDVNDNGPEPYTVPRPCIFRENTPVNQLGTCEIRATDRDTAEFGPPFTMEVSPSFKYSQYLNVIFNANGDGGNGSMTITPLQEFDREAPVPGKILEIPLILADRAGRRNEASVHVIIGDLNDNTMHDGRMTIHVNSYLGRLKETVIGRVYVDDADDWDLGDKTFSWKDSRPGFELSDKGSITMAGEMAAGTYTMSANVHDNARDEDAVGYVTVIVNAVPQIAFDNQGSVQLLIAEETPLQLPDDFIRADSNGQSLMDTFKQEMTAYMGGDVTVDVFSVQVGIATLQTRDVPVLNVRFNARGSTYRDTAQLNGLIAAHRADLQRKLNVEIVGVGIDMCKFTQCDAGCQTLNSADYDGIVVSANSTVIVGVNATSRDDCTCPVWRAPPACQHSLCHNDGVCHNTNPGFFCECRNDGLKGARCQGTTRSFGGNGFAWYKPMPACTSLNISFSFMTTQSDALLFYNGPLETLRNDTHIEYSDYIFIQLRGGRISLEVSMNGQSRSSLEVASTALNDGTWHDISVNQEGKRVELVVDNCRFLGAGADDSSCRAELYTPDDDERLNIVTPVQIGGLAPLSGQDYPQTIPRAGLNGCVRNLNVNGDQYDLATPAFEQNSEKGCRLWGATCDSNSVDSLNHCIHGDCFADVQGSGAMVAKCVCDPGWGGARCERRMEWIQFAQGAFIEYSPRIAFPEQVSDIELLFISGKVNGAPAELSFGTDSQQSYVSTNLESGQNGVTAAGKFDIGTGGRRARQELRVSEVLLKENASYWLQFTRNPTRASLSIDNAYTVSTQLDKGEPFSLQVNQITLGTQGQNKGFQGCIGTYRWSKQNLPLKRGGAMDENEESIVSISNMAGVQDGCDLRITCADLPAGYCGGSFVCVDFWKGPFCTCNDGANAILGDDGQVVGCGETLAVSKLGISSPAIILILVSLALLILLVMMMVVYTRRSPGAFENVRPEEMNRDNLRQYGVEGGGEADNDQYSMAGLRKPVMPLDTGMGPAIGGHPPHYPPRGMAPPKDDHELNSKIKDLETDQNAAPYDELRIYDDERDNISVVTLESIESAQ bar_1-pry_1-2 1015655 n/a 1015657 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1015658 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2 gsk_3-pry_1 1015655 n/a 1015659 unspecified role unspecified role bait bait n/a 1015658 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2 mig_5-pry_1-1 1015655 n/a 1015660 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1015658 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2 apr_1-pry_1 1015655 n/a 1015661 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1015658 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 2 hmp_2-hmr_1 1015655 n/a 1015662 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1015663 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false bar_1-pry_1-1 1015656 n/a 1015657 unspecified role unspecified role bait bait n/a 1015658 unspecified role unspecified role prey prey physical association physical association false false false