UniProt UniProt http://www.uniprot.org gommel-1999-1 p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gommel D., Orci L., Emig E.M., Hannah M.J., Ravazzola M., Nickel W., Helms J.B., Wieland F.T., Sohn K. CoIP experiments with endogenous CHO (Cricetulus griseus) proteins are not included as protein AcNos are not available (fig 3a). Colocalization experiments using coatomer antibody not included as the antibody is not specific to differentiate coatomer subunits (fig 4 a-i). cl6@popix.urz.uni-heidelberg.de FEBS Lett. (0014-5793) 1999 Only protein-protein interactions imex curation gommel-1999-3 p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Gommel D., Orci L., Emig E.M., Hannah M.J., Ravazzola M., Nickel W., Helms J.B., Wieland F.T., Sohn K. CoIP experiments with endogenous CHO (Cricetulus griseus) proteins are not included as protein AcNos are not available (fig 3a). Colocalization experiments using coatomer antibody not included as the antibody is not specific to differentiate coatomer subunits (fig 4 a-i). cl6@popix.urz.uni-heidelberg.de FEBS Lett. (0014-5793) 1999 Only protein-protein interactions imex curation gommel-1999-3 p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gommel D., Orci L., Emig E.M., Hannah M.J., Ravazzola M., Nickel W., Helms J.B., Wieland F.T., Sohn K. CoIP experiments with endogenous CHO (Cricetulus griseus) proteins are not included as protein AcNos are not available (fig 3a). Colocalization experiments using coatomer antibody not included as the antibody is not specific to differentiate coatomer subunits (fig 4 a-i). cl6@popix.urz.uni-heidelberg.de FEBS Lett. (0014-5793) 1999 Only protein-protein interactions imex curation tmeda_rat Transmembrane emp24 domain-containing protein 10 21 kDa transmembrane-trafficking protein Transmembrane protein Tmp21 Tmed10 Tmp21 p24 family protein delta-1 protein protein rat Rattus norvegicus (Rat) Rat MSGLSGPLSWPGPLLSALLFLFLLGPSSVLGISFHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLKITDSAGHILYAKEDATKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE tmed2_rat Transmembrane emp24 domain-containing protein 2 RNP21.4 Tmed2 Rnp24 Membrane protein p24A p24 family protein beta-1 COPI-coated vesicle membrane protein p24 protein protein rat Rattus norvegicus (Rat) Rat MVTLAELLVLLAALLATASGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV tmed2_human Transmembrane emp24 domain-containing protein 2 TMED2 RNP24 Membrane protein p24A p24 family protein beta-1 p24 protein protein human Homo sapiens Human MVTLAELLVLLAALLATVSGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV tmeda_rabit Transmembrane emp24 domain-containing protein 10 TMED10 TMP21 21 kDa transmembrane-trafficking protein Integral membrane protein p23 Transmembrane protein Tmp21 p24 family protein delta-1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MSGWSGPLARRGPGPLALLFLFLLGPSSVLAISFHLPVNSRKCLREEIHKDLLVTGAYEITDQSGGAGGLRTHLKITDSAGHILYSKEDASKGKFAFTTEDYDMFEVCFESKGTGRIPDQLVILDMKHGVEAKNYEEIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE tmed2_crigr Transmembrane emp24 domain-containing protein 2 TMED2 RNP24 COPI-coated vesicle membrane protein p24 p24 family protein beta-1 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster DVLVLLAALLATASGYFVSIDAHAEECFFERVTSGTKMGLIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMTPKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRAINDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV Similar to Q15363 g3hfr3_crigr I79_009427 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MAPTASLGQRRVTWVLPVPARWRPRVVNAAPAPCLVCLAHYVGLALARIAKVEKLKPLEVELRRLEDLSESIVNDFAYMKKREEEMRDTNESTNTRVLYFSIFSMFCLIGLATWQVFYLRRFFKAKKLIE tmed2-tmed10-1 1184189 n/a 1184192 unspecified role unspecified role prey prey n/a 1184193 unspecified role unspecified role bait bait physical association physical association false false false 3c and d tmed10-tmed2-5 1184190 n/a 1184194 unspecified role unspecified role neutral component neutral component n/a 1184195 unspecified role unspecified role neutral component neutral component n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 4o tmed2-tmed10-4 1184191 n/a 1184196 unspecified role unspecified role bait bait n/a 1184197 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B