IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kumagai-2010-3 Treslin collaborates with TopBP1 in triggering the initiation of DNA replication. xenla-oocyte Xenopus laevis oocyte Oocyte anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kumagai A., Shevchenko A., Shevchenko A., Dunphy WG. dunphy@caltech.edu Cell (0092-8674) 2010 Only protein-protein interactions 4 imex curation kumagai-2010-1 Treslin collaborates with TopBP1 in triggering the initiation of DNA replication. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Kumagai A., Shevchenko A., Shevchenko A., Dunphy WG. dunphy@caltech.edu Cell (0092-8674) 2010 Only protein-protein interactions 4 imex curation kumagai-2010-2 Treslin collaborates with TopBP1 in triggering the initiation of DNA replication. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kumagai A., Shevchenko A., Shevchenko A., Dunphy WG. dunphy@caltech.edu Cell (0092-8674) 2010 Only protein-protein interactions 4 imex curation tob1a_xenla DNA topoisomerase 2-binding protein 1-A Cut5 protein DNA topoisomerase II-binding protein 1-A topbp1-A cut5 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MASSENEPFCVKFIKSPENSEYFFKAYEAIKQIQSDESLQLTEEREALLLKEKDKSLYICDPFSGAAFSHLKKLGCRIVGPQVVIFCMENQRRVPRAEYPVYNMAMADVTISCTSLDKETREDVHHYVQIMGGCVYRDLNVSVTHLIAGEVGSKKYLVAASLEKPILLPSWVKELWEKSNQRIIRYSDVNMTEYLCPIFRGCTICVTGLSSLDRKEVQRLTALHGGEYTGQLKMNESTHLIVQEAKGQKYECARKWNVHCISVQWFFDSIEKGFCQDETMYKIEPASTIKSVPDTSTPTGGNSKPNSRTLYDVSQISNISTSCVNESAFNSAMASRLDPPADTLENLDISSLQAPDDLLDGCRIYLCGFGGRKLDKLRKLINNGGGVRFNQLTGDVTHIIVGETDEELKQFLNKTQHRPYVLTVKWLLDSFAKGHLQPEEIYFHSSYQQTEMPSPFEPAINLTANKMSSTRDPLNHTRNHQADEDLLSQYTENNSTLIEDEHPKTSNTNSISQMSMHEDMTTCTSQSGLADTSTIIEGGLFSRKQFMVLGFLEEDEACIIDIIKKSAGKVLSSQKRAIADYAVVPLLGCEVESTVGEVVTNAWLGMCIEQEKLLDPHSNALFTPVPFLEGSTPLRECVLSVSQFMGAERDSLVYLAGLLGAKVQEFFVRKANPKKGMFASTHLVLKDAEGSKYEAAKKWNLPAVTMNWLLQCARTGRKADEDSYLVDNVPEEDKDESFISQTYKPQAIRLSMHAPCHLENHPEALTKAAVTPLDMNRFKSKAFQSVISQHNKNPQTSGGESKVLQREPSLHLDTPSKFLSKDKLFKPSFDVKDALAALETPGGPNQKNRTQSTPLSEVIGRNLQLAIANSTRQTAAVTASPQLKAAEKKEFDNSKLLINVVICVSKKLIKKQGELNGIAASLGAEYRWCFDESVTHFIYHGRQNDMSREYKSVKERSGIYIVSEHWLFACSEQQKRVPEALYPHTYNPKMSLDISAVQDGSYTASKFSADTSLQQDENSELQLQQNNKFGETSDDQVKKAAGDGNPQNPSKDVKGALTQTLEMRENFQRQLQEFMSATSVVKPRGSVGRAGFDNSPCTPEGARSTRNGRSRVLEALRQSRQAMTDLNTEPSQNEQIIWDDPTAREERAKLVSNLQWPDSPSQYSEQLQHNMNDAGGNYTPAKESLTDTEIAELEACEFEPKSAMRTPVIENNLQSPTKPDHLTPTPQAPSIAFPLANPPVAPQPREKPVQPFSKEETLKERRFQLSSLDPQERIDYSQLIEELGGVVIEKQCFDPSCTHIVVGHPLRNEKYLASMAAGKWVLHRSYLEACRAAKRFIQEEDYEWGSMSILSAVTNINPQQRMLAEAAMRWRKKLQGIKQNMGIAEGAFSGWKVILNVDQTKEPGFKRLLQSGGAKVFAGHSSPLFKEASHLFADFSKLKPDEPRVNVAEAAAQGVNCLKPEYIADYLMKELPPPMNNYCLPDAIPYVRVTGTGLSRKRKTSGDVSDVKRSRHY ticrr_xenla Treslin TopBP1-interacting checkpoint and replication regulator TopBP1-interacting, replication-stimulating protein ticrr protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAPSHNVVLLVDTAESSDKSRLRRVSLRLLNFLACRAGLGQVRWSYRFLNSSGGRCRPPRRSDLRELGPRGWEEFEDELEACWERARNCRPSSTQSSRAQLLQTALMETLADFQWDRPDITSPTKPTLLRSRRGRIVAADEPLKDDSPDNFINPHSRNSIFLLSSCPHSGTELGQFAATSGDFSTQKVMDKLLPKSLQKIVSSKRVRVYWLDTSDWTQFGSSSDHSGYWTMVELMHQVEGRILPSESILGSSCQKAKTLPSFSVPPINIPFESVLNYLIFSEPDYQLWFPRRDGILFLTGKDGTKQLDCAVSLEPVSMIQKLSTSLMTIELKGTMQNCNLPLAGLRVETWLLHNSDCVQLQKLTKELMLKELHMIATVTLEDDVLPRTGILSPLSETAAVLNVICSERTLGLDNLHVQGSVHETDKETFSDLPDIVMSVLNHVYSSEDNTLAPDFPVPEWIKQELSQSSRWTSSVTARWYPLSGVSGASCNLMESFRLINAASSNCDEHLKFDQELTNYLSEFYQKKSVDDAGLGVHRENQKKSGLPRTPVRQKMKTLPRALQMLNAARLNVKAQKADSTLPVPNEKNSQMKRRSSGKQDNKPKQLKPTEFQSEDGLISYIKENYEEAVSLVDHSTMTWARDTLTTIKSYLKSIGSEQIETEAIDKVKLLFKTSKVIRQNYRNNQDKEVKLKECQLQVFLRLEMFVQCPVIQMDSDELELIIEEITDILRIVSLTEDPLFLTKFLEVDVLTQYIASVPKILADIYFSLGTQIPEVLVLVLPSDGDDSIMHEEKSVKSQPSTSRVPSVAPIGAETDQLEDLRTRSAKKRRSTALARHRSVAESSQSFRQIEVPKRQPNKENVQSNAVVVLEKLKLPLPAQPQKDAEAKVRRNLFIQETRSPSKRCSKMPRSQSVSAVESLKRKRSKSHDGSKDHHKLLTKKVSETPVHKQTANRLLLRQIKGRPSESNSNISIVEESPEKEIRDIDLRRSPRIKQLSLTRRNSSSFYASQPKSRNLERVNSATQLQQSRERPGSCLISEVKTPKRLLFGEVLGMISPPTTKRSRRILDMVNPVYKTPGKTPRKTPSKNIPNFEDQSGNMLVKSPCTPYTPRTPSRTPKRLKTPSKGSTERKKAAKNLGKLFSPSKPEEKSPLKLWGRRSERLAQMTPGKDGSPYKQSVCQTLMEVKTPQKLQRLESKDFRTPSRTPTRSNNTTPAKQSMQISNTPRKSDLKHPQEHESRGPSGYILWTPQKRILASVPHTPILQTPQKPISASVPRTPVCRTPQKAILSSVPSTPVCQTPNKAILTSVSRTPVYQTPQKAILASVMSTPVYQTPQKPVLASVPTTPILKTPQRSALASVSHTPSPKKYIMKELTVAITRMRECTPEKVLGSNLSSSATPSSALKSFCSEKTTSVCQTPKKSSIALLKPCDSLEFSGAPERLMDSLCSNKDSTKAETACTVPSQISTQMQNVINAGECTDSLSQTSVSSPSIPFTDKSLSPDLKDALSDVTSSKAEGVTIVSEKLDSSSMDSQEATDSFINSSQTEESIDISEARVVSTEASELKMKVLITRKPSGSGVSYLPTTPKCLGNVCSTSTYGLRCTPDRRQREAAARLGTPEIPAKFSTPKSHCKMIPQSIYEVELEMQESGLPKLRFKRTDSNSTIDMDVNKTPKISRKRKGDESPFNEKWCSKHAVRTEPACVSPSCVRTSHYTPGKSGIQTFICQSYTPNRCLSAAASPSQSDAGVPWTPSPKEKLSTDVINSWPRKKKASALCTNLLKCDKIPEYAEEDGGDFELEGVSKLLEKSPVIEQQSKVDGGTFGLRSRKRVFSLVSPTKETENPVKRVCTFNRHEDSSTATHRHQTKEEMEIFSSDQSRSSYLSSSQQSICDDVFNMSDFTPPSKVPKNPLSACGLLTLTQSPLLYKGKTPSSKRKEKIQDVFSDGDSDHGTPTLKRPTNPAAVSDDSPFRKVNPLRSISKTYSRKKLIT topbp1-loc443616-2 Interaction between TOPBP1 and LOC443616 identified by coimmunoprecipitation 885375 n/a 885378 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 885379 unspecified role unspecified role prey prey xenla-oocyte Xenopus laevis oocyte Oocyte physical association physical association false false false Fig. 4A, Supp. Fig. 3 p27 CDKN1B Binding of Treslin to TopBP1 increases substantially near the commencement of DNA replication, triggered by addition of sperm nuclei extracts. topbp1-loc443616-3 Interaction between TOPBP1 and LOC443616 identified by coimmunoprecipitation 885375 n/a 885378 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 885379 unspecified role unspecified role bait bait xenla-oocyte Xenopus laevis oocyte Oocyte physical association physical association false false false Fig. 4A p27 CDKN1B topbp1-loc443616 Interaction between TOPBP1 and LOC443616 identified by coimmunoprecipitation 885376 n/a 885379 unspecified role unspecified role prey prey xenla-oocyte Xenopus laevis oocyte Oocyte n/a 885378 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association false false false Fig. 1A topbp1-loc443616-1 Interaction between TOPBP1 and LOC443616 identified by coimmunoprecipitation 885377 n/a 885378 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection n/a 885379 unspecified role unspecified role prey prey xenla-oocyte Xenopus laevis oocyte Oocyte physical association physical association false false false Fig. 1C, 2D, 2E, 4C p27 CDKN1B