IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fridolfsson-2010-1 UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Fridolfsson HN., Ly N., Meyerzon M., Starr DA. Only interactions observed in at least 3 independent experiments were taken as positive. dastarr@ucdavis.edu [Daniel A. Starr] dastarr@ucdavis.edu Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation fridolfsson-2010-2 UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Fridolfsson HN., Ly N., Meyerzon M., Starr DA. dastarr@ucdavis.edu [Daniel A. Starr] dastarr@ucdavis.edu Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation fridolfsson-2010-3 UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Fridolfsson HN., Ly N., Meyerzon M., Starr DA. dastarr@ucdavis.edu [Daniel A. Starr] dastarr@ucdavis.edu Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation fridolfsson-2010-4 UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Fridolfsson HN., Ly N., Meyerzon M., Starr DA. dastarr@ucdavis.edu [Daniel A. Starr] dastarr@ucdavis.edu Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation fridolfsson-2010-5 UNC-83 coordinates kinesin-1 and dynein activities at the nuclear envelope during nuclear migration. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Fridolfsson HN., Ly N., Meyerzon M., Starr DA. dastarr@ucdavis.edu [Daniel A. Starr] dastarr@ucdavis.edu Dev. Biol. (0012-1606) 2010 Only protein-protein interactions imex curation q23064-3 Nuclear migration protein unc-83 unc-83 Uncoordinated protein 83 W01A11.3 protein protein caeel Caenorhabditis elegans MDGRVTVDSCTSSSEDQMVEQSTNKKWESVLQDVGYSSGENSIHEALNTCADHLVPETSDMRRKRIECSPVKAFYRTVQLEDMSDLEVTKAINHDVEEEPNLSDSMYVNHDSTFLATQNLPEYDEVMALMDDDDLPMDMSMTESFNTKWREIHGQKKPLRRASRPSREQMNLIAKSSCDASSEDSSEGENQTNLEDDPEMMSVSFNSAQFDTSSPLKRQRSARGLKNASFLYDSLEMDGSFCSTRSEMLPPCKTRSLARRKLRVRRMPRSMSDGEQLGVVSSKPEGMMTPMIRVSPPSTPVRRLLRKLDEQIRNRDSDTAPEHSDAAQAYEWDEYNPPQKDDSISDRHIQTMTDISDQLMNIDDDFAEHFGTSSAIRLIEESKSHLRVVLKALEESDSNIPQLSNFELIARSNLRQVDEALKIQSGNQPSFLETSTLQDLRSEWANLYESIRSPFARIMHQVKKFAATLQEVSSMASLGDVDIRSKEDVAKTLDAVTAIERRLSSERQELRDLLASSSFRDVAKDLSCEFESVSEGYDDAVDKIGKMAHSLSQVKGEWDAWNSRQNDIRNAMVRIESHLKEGQMDNKMIADEMELCQERMNSLETMCNYLTASLGSIQNESNSKNLPDFKAELSIYSNALARLKDRFNDMIRVPTPPTVQFHPPEPLPSLARSMTTQTAEMESETENEPLTIAEAISSSRLIKFTFALSLLAALAAIFYYHVFGKPFGPHVTYVNGPPPV klc_caeel Kinesin light chain klc-2 C18C4.10 protein protein caeel Caenorhabditis elegans MSNMSQDDVTTGLRTVQQGLEALREEHSTISNTLETSVKGVKEDEAPLPKQKLSQINDNLDKLVCGVDETSLMLMVFQLTQGMDAQHQKYQAQRRRLCQENAWLRDELSSTQIKLQQSEQMVAQLEEENKHLKYMASIKQFDDGTQSDTKTSVDVGPQPVTNETLQELGFGPEDEEDMNASQFNQPTPANQMAASANVGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAANLLNEALSIREKCLGESHPAVAATLNNLAVLFGKRGKFKDAEPLCKRALEIREKVLGDDHPDVAKQLNNLALLCQNQGKYEEVEKYYKRALEIYESKLGPDDPNVAKTKNNLSSAYLKQGKYKEAEELYKQILTRAHEREFGQISGENKPIWQIAEEREENKHKGEGATANEQAGWAKAAKVDSPTVTTTLKNLGALYRRQGKYEAAETLEDVALRAKKQHEPLRSGAMGGIDEMSQSMMASTIGGSRNSMTTSTSQTGLKNKLMNALGFNS dyl1_caeel Dynein light chain 1, cytoplasmic dlc-1 T26A5.9 protein protein caeel Caenorhabditis elegans MVDRKAVIKNADMSDDMQQDAIDCATQALEKYNIEKDIAAYIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG nud2_caeel Protein nud-2 nud-2 R11A5.2 protein protein caeel Caenorhabditis elegans MDLSEDQIRGLPHHELLGHFLQMREEFNEFQTSSAEIEKMMDSELDDLKTQLKKAETRVQQMTTEQIRNKDRQDDSRVQFAQVEEQLRRENSHLHEQCESQRERIRKLEQRNDVLETSERNKEYLASDLGSKLDHAIEKIAMLESELYERQVAAEEMHRLREEQLRTTERPRLIVEPLRNDPEILPDEPSPGPSKEEFKMSSEDVFMEDVQHHEDVRMEETIAKIDEVRIDDNKNIQEKSQRVSTGTGAGACINRIVKDLMTKVERLDSILSTIRVSNNSSNNNSSHLTTTRA bicd_caeel Protein bicaudal D homolog bicd-1 C43G2.2 protein protein caeel Caenorhabditis elegans MAESELEKLRQDIAILTEKYEQAKEDIHKAANAGLELLRQKEDLEKRLAEMQAELDLARTEIDKTNQTLAEYRSQHQRSTRSELENEESLLEESSAKEEEYLQRIAKLEADLKKKEQELAEKKEELESIEKKHSKEIDSGAALEDERRKLRAELKETKEREQRLISEYSELEEENIGLQKTVANLRGSQVEYESLRIDNNRLEETIEIMKMAAEEDEILRVIADKQLEEALLTAQQERDQRLAMKRELEQTRNAEHISSLNDMLFGLERLGEDGELPPPQPGASDLFSELQGSSDVKVRELEAAKEGLQEELKSREKIFIEFVTGLADTLNIHRPTNELDYMHARQQKDVVLEKIQNIARDTDRHDKEGEEKRSGILKADLRTLVLVAGEKSAQLAAAQDAMIQVSDQLYQFYHQMTQNQGVQTEKSVQEIVKKLRLLARANAEDVPRVSLADEGVESGTETDVNASRSIPLNSDRLVIAPSFAKEIEKKLASVKIGDVLSETDLRQRILTEGNAISETTESLKKMIQVVKRTSEQAFNQAVMASGAENEIEMQNMKLRSLLSTKRDQISTLRTVLKSNKLTAESALTSMREKYESEKKMMMEINDKMRRELKQLKEDAATFASHRAMFTARGEELKSKVEELSNELRANEEEKKTLNQLLRLAIQQKLTLTQRLEEVEVDRDRQVFKRSSTRAPTRETYQPPRAVRYPGSTTTAQQPAPSSSGGSRGGPRRGDNQQ pat12_caeel Protein pat-12 pat-12 Paralyzed arrest at two-fold 12 protein T17H7.4 protein protein caeel Caenorhabditis elegans MTSHIATETSVNRWSTEPVVREEGRPPGPEDFPSTKQGREQDGNVIGITPNYKIGEQKPNSELNLGKTGSLPRGSGDYKMTYRQEDYKQTIIDPKVITNANMPYISTRIPDPRRNLAGDFPSEHSKSKIKLDPLEQRRFRSSESRPITTSNYLAESVDRHREMEASRLREYLKAKENEANQPWNKPGWPGPKKNDESLRELETMKQRIETLKRDANKGPVNAELDELSKRAEELRKRDGWSKYKLVESDIYKTDPDPMPANIKDQVRDLLESRNSVETTTTQRDHDKSGYVTDVSTATWNFSTVDYSPRSVVSMNGASHDILKDDKPRSIMKRNDLVRREQMLYPTVDTQVVKSVVKKPTVTETVQRFEETRRTEEVERRVQRREKKERRSRHHSSSRHHSGWEGHTGGYQGHRSSSLSRGGHGGGGQETYYRQETTRRQQHNNYDDNFNRGIAHARYGSLSDSLRRGELQYVPNGEVRQSFYRDGSNGGQRMHKSYSTRDVFTGDGYDDRRSVSSFRRGSQQQVSPFVEFPPTLPRRGGGGDYRREEDAYFRPVSKSRSYADWDDAGRAGMGREVRRYDDDMSRLEAEFRDSLLMPMPAGNMNERDHRTEQLPGGYETFNKERHANSGRRSGRDGKPVDFNEATQEYNYKREQTLNDDRRRR mysp_caeel Paramyosin unc-15 F07A5.7 Uncoordinated protein 15 protein protein caeel Caenorhabditis elegans MSLYRSPSAALLKSPSQAAFGAPFGSMSVADLGSLTRLEDKIRLLQEDLESERELRNRVERERADLSVQVIALTDRLEDAEGTTDSQIESNRKREGELSKLRKLLEESQLESEDAMNVLRKKHQDSCLDYQDQIEQLQKKNAKIDRERQRVQHEVIELTATIDQLQKDKHTAEKAAERFEAQANELANKVEDLNKHVNDLAQQRQRLQAENNDLLKEVHDQKVQLDNLQHVKYTLAQQLEEARRRLEDAERERSQLQSQLHQVQLELDSVRTALDEESIARSDAEHKLNLANTEITQWKSKFDAEVALHHEEVEDLRKKMLQKQAEYEEQIEIMLQKISQLEKAKSRLQSEVEVLIVDLEKAQNTIALLERAREQLERQVGELKVRIDEITVELEAAQRELRAVNAELQKMKHLYEKAVEQKEALARENKKLHDELHEAKEALADANRKLHELDLENARLAGEIRELQTALKEADAQRRDAENRAQRALAELQALRIEMERRLQEKEEEMEALRKNLQFEIDRLIAALADAEARMKSEISRLKKKYQAEIAELEMTVDNLNRANIEAQKTIKKQSEQLKILQASLEDTQRQLQQVLDQYALAQRKVAALSAELEECKTALDNAIRARKQAEVDLEEANGRISDLISINNNLTSIKNKLETELSTAQADLDEVTKELHAADERANRALADAARAVEQLHEEQEHSMKIDALRKSLEEQVKQLQVQIQEAEAAALLGGKRVIAKLETRIRDLETALDEETRRHKETQNALRKKDRRIKEVQQLVDEEHKNFVMAQDTADRLTEKLNIQKRQLAESESVTMQNLQRVRRYQHELEDAEGRADQAESSLHLIRAKHRSSVVTGKSSSKIFVTEDDY mel26_caeel Protein maternal effect lethal 26 ZK858.4 mel-26 protein protein caeel Caenorhabditis elegans MEPRIDGGVFIGGIGNSGNEMCSNGVPALGVSSQTEIKVEKVQHTWTVKNFSHCYQEYLENFVYLQRGDEQLTWSIKIYPKGNGENNKDFVFLCLNRVINNNVKAGKIGFKSQFKLRTAENKDIEMRIHPNPSHSDYVSYIKRDVLFPQIMPRDMIIVNVEIDVAVETITTTNEPIQFEPTNSEQQLIEDYQRLFSQELLCDFAINVNGKIIRAHKAVLAARSPVFNAMLTHQDTDEAKSSMMYINDMDYDVIYEMVYYIYCGRCNKDITDMATALLIAADKYRLEELKSHCEKYLVENINIENACSLLIIGDLYSAPKLRKRAVTYILARPKNVTGTPGWEDILKGHPNLITDIFSQIDRQSSTGATSSVSNLPGVPMDIPGITGNIVPPPSGL g5ecd7_caeel mel-11 C06C3.1 CELE_C06C3.1 protein protein caeel Caenorhabditis elegans MGQDDEDDAHMLQAKQQITFNVVNKRKDQLRRWQSSEMNAEAARRTKRPKVQFQDSDIFLSACMSGDEEEVEELLNKGANINTCTVDGLTALHQSVIDSKPEMVRFLCEKGADVNAQDNEGWTPLHAAACCGNVAIVRYLCQHGADLSIVNSDKELALDLAVDEQCRDYLDDDYKRQMIDLDACRDQELQTMLKDVNMWMSQGEYRDIPHHRTGGTAMHVAAGRGYTQLLELLIKAGGNVRAQDVEGWTPLHAAAHWAERDACKILLENGAELSDLTFTGADVLGVADKECIDYLVELADTVKVQNKRKSPGSGSQPPTSILQEKNHRMPSHEEHVLTSERKRDLQHKDQHSENEFLHSHPSTASVGSTTSSNTNTTTTTIVIGENDISAAQKDSRESSKESAVDDEEYVAEDEMEEAEEDEEEEDVEEEEEEKSEAELKNGKEISPLRSETTSSRSISITRSLDGYTDRSSSGRETSAEMSEAASSASTGTTSSSSRFTSSTPSSQRSAAGSVHTETPRSSMENSSSVGSGDQNVSATIPIVPLSAPPKAVHQSPSSWINRGVPLSSRSSTSSVTRSSSTPVSEIISPPSSIHSQLSFPSVSNTTTTTATVAPTSSTVPSTPVSNVMAATISSTAKNTPSPAGSVTTINATFSKPSSRHPSEEVYRSPSQPTNVPWASLWHNVNNASTGQIRSHIEQLRNASFSSLSSYPRVSAFRSPIKTTASSTSVNSASSPLLLQPHPHPPVIAEYPRGFVPQQINPNKMSRWQSKTVTESEAERRNNSRMQRQHRRSTQGVTKEQLEEASKFATDEVARRNSQVLSTYPNSLHARLASEEKDLSSTSDDLKDGDVSTTATVSLINSPNSNSANVRRKSQGLSISRSNRRATGPVNPEDLAPAMSLRLATAQPYSPASVRSNAGTLPTMTSRFSEKAQSLPPASNGMSSTQPLSTRSAIVAGLSSAPSMRTTAPLSSTATSSRFMPSSSLSSNGSSANQQPSSIYGLQSRPTETNLNYKALFEKERSECERLRREMEEMRRSQTADSYRGASTQLAWRARNASPSAQPHNLSVAKSTSLASFDENERRSMERKISDLELQLKTATNLRMENQRLKEENGALVRVISKMTI h2kz24_caeel C27H5.2 CELE_C27H5.2 protein protein caeel Caenorhabditis elegans MSSITGLQVKSLRIGVEPVEVELDEDAGILWTSLQTAFPGCSGMYYREIGDSCKKSVKFDGKKFMAPGGAWNDREYFVTLSQRCHTAAGNSGHTASYSDATKQFEKSVQAVQKMMLASGMKIDMSHLTKKRQHTSSRETASSTSSSTVTPDPIQAIEERSQMLKTAGRELSPLEQQFLDLCRISTAKDQIIDANRQEITKMNETLRILEKNLKETETNLSEANAMVDCKQQELEMVSEALKRLEESRHTISELNRKSGNLEENVTELQMKLMNVEEENHGLKAQLETSHNRIKEMSDTISMLTSSKDILSKELDQLRPFADAAGIEDYENIPTFIHAIQENKELRKTNENMKLQSEETKDNHKQMQSIHTEVFEENAKLRNRNEDLERRFAHVDDELACVNENWAKELKEKQQDWDKVKYAAESEVLSLREQLVHLQSILESATREAAENARRVSELEHERHEVRRLSDAMHIKAQEDLARQVRVLKERLAESDSQVAELNVLVSSLAADATASRREISDLL ylu3_caeel Uncharacterized protein F10E9.3 F10E9.3 protein protein caeel Caenorhabditis elegans MASKTRRGSSLAKKEKEIKKEIEEDKEVAPIQEEDDDGPPPILEREASCGENDDTDGDGKEEDAQKEDDIDGSKTKNESILEALSQFFTADDATVKNRRNSPPSESSSTRNTLGLNRGGAIPKALLLNKNLRPVPSKTPPIATTRFASLRHLSSAPGTSASTSGSPSVGIRKYGVPPPMPRSLRNIAGSINVVGSNSSLGPARLLESRKRHFDKDKDGKNPKRSSLVAYNGGRAGENFRKVPPSAVSPAPGSGSSASSGTSTSSSNGIIDVQSMLRDQSPVRMQVMVGGDHETNGDSIQEAMELANAQKESIGNIARASSHHNMLLGSMIINGLNTLQSRDKDEYTNFSSDLFSLITRYNIN o76640_caeel math-41 T08E11.4 CELE_T08E11.4 protein protein caeel Caenorhabditis elegans MANQDQNYLGPRTHPPNLQKSTSFSGAKLHYQYQYTGKEQKTSSSKDESRVHHSESRSPRLSKHLAEDDGSGSTLDQRLSRQLTVDYGGGSTVDQRSQLGTSGTKYLTTVQSSNLDSAASPLSFSGHTAPSKIAVVRETLPVSSQASIFPTSYRSSQYPFLNPSTKVTTTPPIQQSHLGTSRLTTVQSSNLDRSPSPSRSRYMNSRDRRKTTTIIPSSIPQSSEYKSAFQREAPAVYFNQHTESQSTYKPLPMSSQTQNRNLTIGYTPNQFQEIERAQREFKNQKKQEEEIRAKLDRRRIEESQRLEAERFALEATMVDEDEDVAKFIRNPEERFKDSRDSKKLEWRSRRAPNQPEQVCGESCENAKLGKFGKFAKLSKYGKFARLGKFGKFATLGKFSKYYIRTMPNSCKEFSISENVKNISRFVDGERYHTNLEDRFNIPWRIRIEKTNDNFQLYLRCEKEECENRKWSIETEITLKLVSHNGKSLMKNWKYTFAKPTGRGFEFISWKELESDYVVDDSIVVEAHVKILNILDSPCTETMNQKTFMLNHTVQNVSNIKERGIYTTNVETHYNIPWRIEIQRREGFFGMYLQCEKKHFDSKDWSIEVELVLTLKSSNGQRLSFTASSTLNEPASYGYDKLIRWDDMERKYAVNDSIIIEARVKIIKMTGFEERPPVGRLSGIVIPVGDREYSMEEWMEMAFGC h2kyj3_caeel bath-44 CELE_C07D10.2 C07D10.2 protein protein caeel Caenorhabditis elegans MDNSLMQMRHAADSWSTSEVRSLVHKHMWTIRGFSQLECRYLETSAKIKDITKDESGLQVPLTHQPSSSGGLPELPDITFRIRLHPQGNKESNKDFTFFQCFTNQATTTTTPTFRAKFKFTVFNQRTEETPTTVYSGTQQLHGYFEYIRREVLVGHVQPADDLILCLTITITFDTVTKAAQNTRSVPPELPMPSDVTKDLENLYRSGKHADFTLVVEERELKAHKAILAARSPVFAAMMEPHTAEAQNSRAILRDIDYEVVQAILYYIYTGTCTNMGGNALDILAAAERFALPGLKNIAEVAMRNGLATETVCKNLAFAEMYGLVDFKKEAIKYICMNANAVINSEGFLRLSRQNPDLIVDIMTVLVNERHNSPSCFSLDGTLEPASKRARMTETNL ess2_caeel Splicing factor ESS-2 ess-2 ES2 similar protein 2 F42H10.7 protein protein caeel Caenorhabditis elegans MSSFDKNDERAQLMVPKSINEGKVVKLSQKTLVTKKIERQVVPEEKYIAGLDKIIEKDYFPHLKKMQAQKEYLEAVANKDINKIKELQMKFCSTGSVRTDRSFRTPITTRSTTEAPDVSSFDADTPGPSSASTSSAHDWMQSPMPFANEEGDNEALNRKRKKKKEETLTSYLNKYTSEDNASFEELAKVMREREDARRPWVYKAEEEHNKNLVTRQAIAAEADVQLALKHAVDADDNRPLNVDNWAYKAWNTVLFNPDGAALTPAEIADAARKQQTEINKRGTRFPDSGKLKPSDEAMTRAAVSHALANAGKVDFLGNEVTPANSFKLLETPNPNPDDMDSPLMTWGEIDGTPFRLDAPDVTEHSLPGAAPVFKIPEVPYREKIAQSMNDSIAAKYRDKRKVAMRAAEGAHFSRTPGFGSKRVSDKLAQLSPAAQKLATKKLGLKMLPAHKSPFASPKIMSNWSRPSSSKRSTTPGSAWSRGSTTPGSSWSQGAQTPGTPGIESMIRRKGDNVGPSTSAGAADRANAGDFF q5fc73_caeel F54D5.5 CELE_F54D5.5 protein protein caeel Caenorhabditis elegans MHYLVFFDLLFKPPITLSPIDLPTFPVHISLSVKVSATHSIGPHVLPIDHPVRLALISHSADTTKMAAIIRTLFKSKKSKKGDEENNEDLAREVSNRRSFRAPSTSSSKSQRRAVARFADDGALPATAAYHTTSAPKLRKGPQSCPGGYRDESDVSYESDKYSQKSNSHRRRDVLHQSMRQNYTIDEGRKPSSRRHHSSKSDFRRRGESSEYGSGDPSPVGQYSSRHSHYEDRIEESENDDFSEKAQFAVIERAQQHYMQKYKESEQKRREEKRKLKDMEKECNDAKNAMFHYMNQLDKVKKERDCYRKELSRCKKQLEAFQNSGYDAFHHLRQPQISNPQQYPPPLSMGSYPYMSQSMNASHHMSSNFMNSAPPSSNTPMTMTSGAGESLINPSDVSFLQNSFLQPTSQGLTGMTPPPGGFKVPTNYGDDDRDEVKDYRSELLIETSRSTKEVDSISPSSSADSQVTLLDHSTIDKENRKPPSRPFSMMSI egal1_caeel Egalitarian protein homolog egal-1 C10G6.1 protein protein caeel Caenorhabditis elegans MEEAKNMALLFFMDHLMQKNGRRTIHDLSCQFGARGFSEEMRNAVGTTQEGLTEFLQGHPSLFTVEGDQVILNGHNDLNAKNNPLLQSGIRSRNYEKEAVDFFVTKLTKFGPELQIKSLLGHRSQAAPEVRLVSGRHLKEFCEFLQSQVDYFVVEGDRVRLKNMPEPDENAIEMDDEGRPLAGVKAKQAAVEYLKSVLEQNEDQPIPLDQFYQNFCQRFSHTIRQDVATNPKELLQFLKLNRGLFFIRSNKVSLVKNRLNEDGSENGSDEGEETNNNGMFPLDQSALTRIHFVKALKPAQDLISRLWQDINNMEKKVVGLDLKTVTVGVDGEIFLSLGVIATTSQIGIFDLASSDVIILESGFKGILESEKVVKVIHDARRVASLLAHKYAVHMRNVFDTQVAHSLLQHEKFNKSLNEMRPISFINLQRVYYPQSIMLSDVTPRKMSMCPNWGVRPITEEFQLTIVEEAHCLLSALYQSLSNLIPVHLRGVFEDKCIEVNHPEVLLASPNRPPPQPFISSPYRASTRRDVRNGGSIMQSFSPAPYAAAPRPQMSDACTQTFSTGDIEVLNVFYE unc83_caeel Nuclear migration protein unc-83 unc-83 Uncoordinated protein 83 W01A11.3 protein protein caeel Caenorhabditis elegans MDVMDSFSEVEMPNDISSEDHLLKVIESSAEEVDIFLENCSSLYNLILDSLHNLTSKTISCECLDEMTSTLEKSAKKILAERPEAENSVLLRLNTICCAMDQLRVQHNSRMMSGADSDTASSARSSTSSSTGEMRLWLHEVERRLEINEKRIRVEPNLQLLLSDQQALQLEIQHEGQLLVNRLNKQIKDDHDSDSSEEEKRKTCVDAIRKRWHTIYLNSLSLVCRIEELINHQQASEDSESDPDLVGPPIKRARIRTVGHLTASDTEESEADEEDRHSQTETVVTEDDNVLPFAENEYESIMDGRVTVDSCTSSSEDQMVEQSTNKKWESVLQDVGYSSGENSIHEALNTCADHLVPETSDMRRKRIECSPVKAFYRTVQLEDMSDLEVTKAINHDVEEEPNLSDSMYVNHDSTFLATQNLPEYDEVMALMDDDDLPMDMSMTESFNTKWREIHGQKKPLRRASRPSREQMNLIAKSSCDASSEDSSEGENQTNLEDDPEMMSVSFNSAQFDTSSPLKRQRSARGLKNASFLYDSLEMDGSFCSTRSEMLPPCKTRSLARRKLRVRRMPRSMSDGEQLGVVSSKPEGMMTPMIRVSPPSTPVRRLLRKLDEQIRNRDSDTAPEHSDAAQAYEWDEYNPPQKDDSISDRHIQTMTDISDQLMNIDDDFAEHFGTSSAIRLIEESKSHLRVVLKALEESDSNIPQLSNFELIARSNLRQVDEALKIQSGNQPSFLETSTLQDLRSEWANLYESIRSPFARIMHQVKKFAATLQEVSSMASLGDVDIRSKEDVAKTLDAVTAIERRLSSERQELRDLLASSSFRDVAKDLSCEFESVSEGYDDAVDKIGKMAHSLSQVKGEWDAWNSRQNDIRNAMVRIESHLKEGQMDNKMIADEMELCQERMNSLETMCNYLTASLGSIQNESNSKNLPDFKAELSIYSNALARLKDRFNDMIRVPTPPTVQFHPPEPLPSLARSMTTQTAEMESETENEPLTIAEAISSSRLIKFTFALSLLAALAAIFYYHVFGKPFGPHVTYVNGPPPV unc83c-klc2-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975002 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 20 Table 1 20 unc83c-dlc1-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975003 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 4 Table 1 4 unc83c-nud2-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975004 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 4 Table 1 4 unc83c-bicd1-1 974996 n/a 975005 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 3 Table 1 3 unc83c-gei16-1 974996 n/a 975006 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 12 There are 12 isoforms for gei-16 (Q95QA6) in UniProtKB/TrEMBL. It is unclear which isoform is identified in the screen so I have used isoform d as this is the longest isform and will be the master entry when the entries are merged. Table 1 12 unc83c-unc15-1 974996 n/a 975007 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 5 Table 1 5 unc83c-mel26-1 974996 n/a 975008 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 6 Table 1 6 unc83c-mel11-1 974996 n/a 975009 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 5 There are 4 isoforms for mel-11 (Q8I0N9) in UniProtKB/TrEMBL. It is unclear which isoform is identified in the screen so I have used isoform c as this is the longest isform and will be the master entry when the entries are merged. Table 1 5 unc83c-c227h5_5-1 974996 n/a 975010 unspecified role unspecified role prey prey n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 10 There are 4 isoforms for C27H5.2 (Q18266) in UniProtKB/TrEMBL. It is unclear which isoform is identified in the screen so I have used the longest isoform as this will be the master entry when the entries are merged. Table 1 10 unc83c-f10e9_3-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975011 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 5 Table 1 5 unc83c-math41-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975012 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 3 Table 1 3 unc83c-bath44-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975013 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 3 There are 2 isoforms for bath-44 (Q95QX2) in UniProtKB/TrEMBL. It is unclear which isoform is identified in the screen so I have used the longest isoform as this will be the master entry when the entries are merged. Table 1 3 unc83c-ess2-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975014 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 3 There are 2 isoforms for ess-2 (P34420) in UniProtKB/Swiss-Prot. It is unclear which isoform is identified in the screen so I have used isoform b as this is the longest isform and the master entry. Table 1 3 unc83c-f54d5_5-1 974996 n/a 975001 unspecified role unspecified role bait bait unc83c_137-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975015 unspecified role unspecified role prey prey physical association physical association false false false author score author-based confidence author score A score generated by an author, usually only relevant for that particular dataset. 3 There are 2 isoforms for F54D5.5 (Q5FC73) in UniProtKB/TrEMBL. It is unclear which isoform is identified in the screen so I have used the longest isoform as this will be the master entry when the entries are merged. Table 1 3 dlc1-egal1-2 974997 n/a 975016 unspecified role unspecified role bait bait n/a 975003 unspecified role unspecified role prey prey dlc1_1-89 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5. dlc1-unc83-2 974997 n/a 975017 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975003 unspecified role unspecified role prey prey physical association physical association false false false Fig. 5. bicd1-unc83-2 974997 n/a 975005 unspecified role unspecified role prey prey n/a 975017 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5. bicd1-egal1-2 974997 n/a 975005 unspecified role unspecified role prey prey bicd1_253-737 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975016 unspecified role unspecified role bait bait physical association physical association false false false Fig. 5. unc83c-nud2-2 974997 n/a 975004 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975001 unspecified role unspecified role bait bait unc-83c_362-692 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 975094 975092 physical association physical association false false false Fig. 6A unc83c-bicd1-3 974998 n/a 975005 unspecified role unspecified role bait bait gst-bicd1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 975001 unspecified role unspecified role prey prey mbp-unc83c mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 975101 975098 physical association physical association false false false egal1-bicd1-3 974998 n/a 975016 unspecified role unspecified role prey prey mbp-egal1 mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 975005 unspecified role unspecified role bait bait gst-bicd1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false bicd-1_227-737 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5D unc83c-dlc1-3 974998 n/a 975003 unspecified role unspecified role bait bait gst-dlc1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 975001 unspecified role unspecified role prey prey mbp-unc83c mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 5B egal1-dlc1-3 974998 n/a 975003 unspecified role unspecified role bait bait gst-dlc1 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 975016 unspecified role unspecified role prey prey mbp-egal1 mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5B unc83c-nud2-3 974998 n/a 975004 unspecified role unspecified role bait bait gst-nud2 gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nud2_239-293 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) n/a 975001 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 975125 975122 physical association physical association false false false Fig. 6B unc83c-nud2-4 974999 n/a 975001 unspecified role unspecified role prey prey myc-unc-83c myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false unc-83c_1-698 unc-83-KASH sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 975004 unspecified role unspecified role bait bait gfp-nud2 gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 7A unc83c-nud2-5 975000 n/a 975004 unspecified role unspecified role neutral component neutral component gfp-nud2 gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 975001 unspecified role unspecified role neutral component neutral component myc-unc-83c myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false Fig. 7B