IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ uhlenhaut-2009-1 Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. mouse-ovary Mouse ovary ovary Ovary ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Uhlenhaut NH., Jakob S., Anlag K., Eisenberger T., Sekido R., Kress J., Treier AC., Klugmann C., Klasen C., Holter NI., Riethmacher D., Schutz G., Cooney AJ., Lovell-Badge R., Treier M. treier@embl.de Cell (0092-8674) 2009 Only protein-protein interactions 3 imex curation uhlenhaut-2009-2 Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Uhlenhaut NH., Jakob S., Anlag K., Eisenberger T., Sekido R., Kress J., Treier AC., Klugmann C., Klasen C., Holter NI., Riethmacher D., Schutz G., Cooney AJ., Lovell-Badge R., Treier M. treier@embl.de Cell (0092-8674) 2009 Only protein-protein interactions 3 imex curation uhlenhaut-2009-3 Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. mouse-ovary Mouse ovary ovary Ovary fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Uhlenhaut NH., Jakob S., Anlag K., Eisenberger T., Sekido R., Kress J., Treier AC., Klugmann C., Klasen C., Holter NI., Riethmacher D., Schutz G., Cooney AJ., Lovell-Badge R., Treier M. treier@embl.de Cell (0092-8674) 2009 Only protein-protein interactions 3 imex curation sox9_mouse_gene Mouse Sox9 gene gene gene mouse Mus musculus Mouse foxl2_mouse Forkhead box protein L2 Foxl2 Pfrk Pituitary forkhead factor protein protein mouse Mus musculus Mouse MMASYPEPEDTAGTLLAPESGRAVKEAEASPPSPGKGGGTTPEKPDPAQKPPYSYVALIAMAIRESAEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEKGNYRRRRRMKRPFRPPPAHFQPGKGLFGSGGAAGGCGVPGAGADGYGYLAPPKYLQSGFLNNSWPLPQPPSPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYSRVQSMALPPGVVNSYNGLGGPPAAPPPPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL esr1_mouse Estrogen receptor Esr1 Esr Estr Estra Nr3a1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein mouse Mus musculus Mouse MTMTLHTKASGMALLHQIQGNELEPLNRPQLKMPMERALGEVYVDNSKPTVFNYPEGAAYEFNAAAAAAAAASAPVYGQSGIAYGPGSEAAAFSANSLGAFPQLNSVSPSPLMLLHPPPQLSPFLHPHGQQVPYYLENEPSAYAVRDTGPPAFYRSNSDNRRQNGRERLSSSNEKGNMIMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDLEGRNEMGASGDMRAANLWPSPLVIKHTKKNSPALSLTADQMVSALLDAEPPMIYSEYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFGDLNLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRMGVPPEEPSQTQLATTSSTSAHSLQTYYIPPEAEGFPNTI esr2_mouse Estrogen receptor beta Esr2 Estrb Nr3a2 Nuclear receptor subfamily 3 group A member 2 protein protein mouse Mus musculus Mouse MEIKNSPSSLTSPASYNCSQSILPLEHGPIYIPSSYVESRHEYSAMTFYSPAVMNYSVPSSTGNLEGGPVRQTASPNVLWPTSGHLSPLATHCQSSLLYAEPQKSPWCEARSLEHTLPVNRETLKRKLGGSGCASPVTSPSAKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRIVRRQRSASEQVHCLNKAKRTSGHTPRVKELLLNSLSPEQLVLTLLEAEPPNVLVSRPSMPFTEASMMMSLTKLADKELVHMIGWAKKIPGFVELSLLDQVRLLESCWMEVLMVGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTARFRELKLQHKEYLCVKAMILLNSSMYPLATASQEAESSRKLTHLLNAVTDALVWVISKSGISSQQQSVRLANLLMLLSHVRHISNKGMEHLLSMKCKNVVPVYDLLLEMLNAHTLRGYKSSISGSECCSTEDSKSKEGSQNLQSQ dmrt1_mouse Doublesex- and mab-3-related transcription factor 1 Dmrt1 protein protein mouse Mus musculus Mouse MPNDDTFGKPSTPTEVPHAPGAPPQGKAGGYSKAAGAMAGAAGGSGAGGSGGASGSGPSGLGSGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCSLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNASNPCLMAENSSSAQPPPASTPTPAASEGRMVIQDIPAVTSRGHMENTSDLVSDPAYYSSFYQPSLFPYYNNLYNYPQYSMALSAESSSGEVGNSLGGSPVKNSLRSLPAPYVPAQTGNQWQMKTSESRHPVSSQYRMHSYYGPPSYLGQSMSQIFTFEEGPSYSEAKASVFSPPSSQDSGLVSLSSSSPMSNESSKGVLECESASSEPSSYAVNQVLEEDEDE sox9_mouse Transcription factor SOX-9 Sox9 Sox-9 protein protein mouse Mus musculus Mouse MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQAGKVDLKREGRPLAEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAPTPATAGHVWMSKQQAPPPPPQQPPQAPQAPQAPPQQQAPPQQPQAPQQQQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQISYSPFNLPHYSPSYPPITRSQYDYADHQNSGSYYSHAAGQGSGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP foxl2-sox9_gene-1 871303 n/a 871306 unspecified role unspecified role prey prey n/a 871307 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false Fig. 5C FOXL2 binds to the cis-regulatory element for testis-specific Sox9 expression, referred to as TESCO. esr1-sox9_gene-1 871303 n/a 871308 unspecified role unspecified role bait bait n/a 871306 unspecified role unspecified role prey prey association association false false false Fig. 6A ESR1 binds to the cis-regulatory element for testis-specific Sox9 expression, referred to as TESCO. foxl2-esr1 Interaction between Foxl2 and Esr1 demonstrated by coimmunoprecipitation 871304 n/a 871307 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 871308 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6D foxl2-esr2-1 Interaction between Foxl2 and Esr2 demonstrated by coimmunoprecipitation 871304 n/a 871309 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 871307 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 6D sox9-dmrt1-1 Colocalisation of Sox9 and Dmrt1 871305 n/a 871310 unspecified role unspecified role neutral component neutral component n/a 871311 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 10