MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint ren-2009a-2 Structure and function of a novel endonuclease acting on branched DNA substrates. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation ren-2009a-3 Structure and function of a novel endonuclease acting on branched DNA substrates. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation ren-2009a-1 Structure and function of a novel endonuclease acting on branched DNA substrates. in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation ren-2009a-4 Structure and function of a novel endonuclease acting on branched DNA substrates. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation ren-2009a-5 Structure and function of a novel endonuclease acting on branched DNA substrates. in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Ren B., Kühn J., Meslet-Cladiere L., Briffotaux J., Norais C., Lavigne R., Flament D., Ladenstein R., Myllykallio H. EMBO J. (0261-4189) 2009 Only protein-protein interactions imex curation nucs_pyrab Endonuclease NucS nucS Nuclease for ssDNA PAB2263 PYRAB01260 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MRKVIIKENPSEEEIKELLDLAEKHGGVVTIFARCKVHYEGRAKSELGEGDRIIIIKPDGSFLIHQNKKREPVNWQPPGSKVTFKENSIISIRRRPYERLEVEIIEPYSLVVFLAEDYEELALTGSEAEMANLIFENPRVIEEGFKPIYREKPIRHGIVDVMGVDKDGNIVVLELKRRKADLHAVSQLKRYVDSLKEEYGENVRGILVAPSLTEGAKKLLEKEGLEFRKLEPPKKGNEKRSKQKTLDFFTP mint top6b_pyrab Type 2 DNA topoisomerase 6 subunit B top6B Type II DNA topoisomerase VI subunit B PAB0407 PYRAB05940 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MAEARDLFKEFKVQSVSEFFRRNAAMLGYTGKIRSLTTIIHEAVTNSLDACEEAGILPYIRVEIEELGKEHYKVIVEDNGPGIPEEYIPHVFGKMLAGTKAHRNIQSRGQQGIGISGAVMFAQITSGKATRVITSTGGEIVEAWVKIDVQKNEGKIVKKLKHPNPKGWRGTRIELEVKDVKYVRSKQGVYWYLKLTAIANPHAHIELVEPDGKLVVFPRSSEDIPEPPVEMKPHPKGVMVDDVYTMAHRSKRSSVRRFLVSEFSRISDKKVDELIKYITALRLIKSETNKEVKEKLYEKLVSGDVDSVLRAFGRKWKKELEKVAKIMDKSPEKLTWHEAEEIVEAFKLMKFLAPPTHGLRPIGEENIKKGLTSILKPEFVTAVTRAPRVYAGGIPFQVEVGLAYGGQIQGSEILRYANRVPLLFDAGSCVITSAVRSIDWKRYKIDSFDSAPLVVLVNVVSVHVPYTSTGKQSIADIDEIHNEIRLALMDAARRLSFYLGGKFRRMYQVKRRKTLEKYLPEIARSLHILTGEPEEKIKEYFLKLIESKIEVEEVSEVEAEEAEA mint rfcs_pyrab Replication factor C small subunit rfcS Clamp loader small subunit PabRFC small subunit PAB0068 PYRAB01100 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MRDMEEVREVKVLEKPWVEKYRPQKLEEIVGQEHIVKRLKHYVKTGSMPHLLFAGPPGVGKCLTGDAKVIANGELTTIGELVERISNGKLGPTPVRGLTVLGIDEDGKLVELPVEYVYKDKTSELVKIRTRLGRELKVTPYHPLLVNRRNGKIEWVKAEELKPGDRLAIPSFLPAMLNDNPLAEWLGYFFGNGYTDSEERVVFESKSKELRKRFMELTRKLFQDAEIKEDSGKVYVSSSEVKRLVKSLNKDSIPEQAWKGLRSFLRAYFDCNAEIKDKIIVSTAGKEIAEQISYALAGLGIVAEVDDKGSVIISDPENVSRFLDEIGFSVEEKKEEAKALIKKSTLNLGIYVDKELISYVREKLKLSFYENETMWSPEKAREIAWKLMKEIYYRLDELERFKKALSKSVIIDWSEVEKKKEEISEKTGISVNEILEYAKGKRKPSLEEYVKIAKALGVELKETLEAIFTFGKKYLGYVISDEIETLEEVRKEELKRLKELLNDEKLKKGVAYLIFLAQNELLWDEIIEVEKLKGDFVIYDLHVPKYHNFIGGNLPTVLHNTTAALALARELFGENWRHNFLELNASDERGINVIREKVKEFARTKPIGGASFKIIFLDEADALTQDAQQALRRTMEMFSSNVRFILSCVTGDTKVYTPDEREVKIRDFMNYFENGLIKEVSNRIGRDTVIAAVSFNSRIVGHPVYRLTLESGRIIEATGDHMFLTPEGWKQTYDIKEGSEVLVKPTLEGTPYEPDPRVIIDIKEFYNFLEKIEREHNLKPLKEAKTFRELITKDKEKILRRALELRAEIENGLTKREAEILELISADTWIPRAELEKKARISRTRLNQILQRLEKKGYIERRIEGRKQFVRKIRNGKILRNAMDIKRILEEEFGIKISYTTVKKLLSGNVDGMAYRILKEVKEKWLVRYDDEKAGILARVVGFILGDGHLARNGRIWFNSSKEELEMLANDLRKLGLKPSEIIERDSSSEIQGRKVKGRIYMLYVDNAAFHALLRFWKVEVGNKTKKGYTVPEWIKKGNLFVKREFLRGLFGADGTKPCGKRYNFNGIKLEIRAKKESLERTVEFLNDVADLLREFDVDSKITVSPTKEGFIIRLIVTPNDANYLNFLTRVGYAYAKDTYARLVGEYIRIKLAYKNIILPGIAEKAIELATVTNSTYAAKVLGVSRDFVVNRLKGTQIGITRDFMTFEEFMKERVLNGYVIEKVIKKEKLGYLDVYDVTCARDHSFISNGLVSHNCNYSSKIIEPIQSRCAIFRFRPLNDEDIAKRLRYIAENEGLELTEEGLQAILYIAEGDMRRAINILQAAAALDRKITDENVFLVASRARPEDIREMMLLALKGNFLKAREKLREILLKQGLSGEDVLVQMHREVFNLPIDEPMKVYLADKIGEYNFRLVEGANEMIQLEALLAQFTLIGKKK mint pcna_pyrab DNA polymerase sliding clamp pcn Proliferating cell nuclear antigen homolog PAB1465 PYRAB13790 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MPFEIVFEGAKEFAQLIETASRLIDEAAFKVTEEGISMRAMDPSRVVLIDLNLPASIFSKYEVDGEETIGVNMDHLKKVLKRGKAKETLILRKGEENFLEISLQGTATRTFKLPLIDVEEIEVDLPELPFTAKVVILGDVIKEAVKDASLVSDSMKFIAKENEFTMRAEGETQEVEVKLTLEDEGLLDIEVQEETKSAYGISYLSDMVKGLGKADEVTIKFGNEMPMQMEYYIRDEGRLIFLLAPRVEE mint q9v1z5_pyrab PAB0190 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MNTMYLRKELLQPRLYQELIYAKCKERNCLIVLPTGLGKTIIAMMIADYRLNKYGGKVLMLAPTKPLVLQHAETFRKFFNLPPEKIVALTGEVSPSERVKAWNRAKVIIATPQTVENDLLTGKINLEDVVLVIFDEAHRAVGNYAYVYIAREYLNQAKNPHVIGLTASPGSSQEKILEVLRNLGIEHIEYRSENSPDVKPYVQGIKFEWIKVELPGLYKEVRKLLRDMLRDSLKPLAEAGLIESASADLPKKEILRAGQIINEETAKGNHDLRKLLLFHAMALKLHHAIELLETQGLSALRVYLKKLYEEAKTGSTRASKELFLDRRMKKALALLIQAKELGIDHPKMEVLKELVKEQLSKKENSKIIVFTNYRETARKVVEELTKEGIKAKRFVGQATKENDRGMSQREQKLILDSFARGEFNVLVATSVGEEGLDVPEVDLVVFYEPVPSAIRSVQRRGRTGRQKPGRVVILIAQGTRDEAYYWSSKHKERQMRETIRMVSQAIKREKQLSLESYVKKDTEKEMEREEKEERKGEREGVKVVVDSRELRSEVVKRLKTLGVKIEVKTLDVGDYIISDEVAIERKSANDFIQSIIDGRLFDQVKRLKDSYPRPVVIVEGQLYGIRNVHPNAIRGAIASIVIDFGVPIIFSSNPEETANYIFFMARREQEERRREVRVRGEKKALTLAERQRLIVESLPHVSATLAKRLLKHFGSVERVFTASVPELMKVEGIGEKIAREIRRVITSPYTED mint rfcl_pyrab Replication factor C large subunit rfcL Clamp loader large subunit PabRFC large subunit PAB0069 PYRAB01110 protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MPEVPWVEKYRPRRLSEIINQEDAISKVKAWIEAWLHGNPPKKKALLLAGPPGSGKTTTVYALAREYNFEVIELNASDERTYDKIARYVQAAYTMDILGKRRKIIFLDEADNIEPSGAPEIAKLIDRARNPIIMAANHYWEVPKEIRDRAELVEYKRLTQRDVINALVRILKREGITVPKEVLVEIAKRASGDLRAAINDLQTVVAGGYEDARYVLAYRDVEKTVFQALGLVFASDNAKRAKLAMMNLDMSPDEFLLWIDENIPHMYLKPEEMARAYEAISKADIYLGRAQRTGNYSLWRYAIDMMTAGVAVAGTKKRGFAKFYPPNTLKMLAESKEERSTRNSIIKKIMSEMHMSKLEAIETMKIIKTIFEKNLDLAAHFTVFLGLSEKEVEFLAGRENAGTIWGKALAIRRKLKKEEEKIRKERKEEEKVEVREEKPEEKVEEKREERETKKEKEKKEEKKAEKKGKQVTLFDFIKK mint top6a_pyrab Type 2 DNA topoisomerase 6 subunit A Type II DNA topoisomerase VI subunit A PAB2411 PYRAB05950 top6A protein protein pyrab Pyrococcus abyssi (strain GE5 / Orsay) MKLKRQKPKEKFSYDPQKVLKKLEDLAWKILEEVKSGKNPYFDVPTRGLNNVYFDEEARLIKLGDKLSRRYFLNVAHARKFTQTLILMAYIKRLVSEGKHASLREAYYANKHTIPGTRENTFEDQSESDPIIEDLERMLGVLREEMHITADRRGYIYGDIVIKDGEDEFNASKLGTGGWAVPGTVEHIQFPEVNVDYVLVVETAAMADRLIEEKYPKKENCLIVATQGQASRGVRRLIHRLHYEEGLPIIVFTDGDPYGWYIYSTIKQGSINLAYLSEKLATPDAKFVGMTMDDIKEYNLEHVTEKLKGIPPDKKGGPTGDYKRLIEELNYPWFQNKEWQRQLKLALKWGVRIEQQALANKSLEFVAKEYLPEKIREGKLLP mint nucs-pcn 922882 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false top6b_pyrab DNA topoisomerase 6 subunit B 922888 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rfcs_pyrab Replication factor C small subunit 922889 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level pcna_pyrab PCNA 922890 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level q9v1z5_pyrab Hef 922891 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rfcl_pyrab Replication factor C large subunit 922892 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level top6a_pyrab DNA topoisomerase VI subunit A 922893 ms participant Identification by mass spectrometry ms participant unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false sf1 mint nucs-pcn-1 922882 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false pcna_pyrab PCNA 922890 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false f1c mint nucs-2 922883 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false f2d mint nucs-2 922883 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 4.0 direct interaction direct interaction false false false f2d mint pcn-nucs 922884 pcna_pyrab PCNA 922890 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false 922884 f1c mint nucs-2 922885 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false f1e mint nucs-2 922886 y126_pyrab NucS 922887 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false f1d mint