IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kloeker-1999-1 Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. bovin-testis Bos taurus testis chromatography chromatography technology column chromatography chromatography fingerprinting peptide massfingerprinting fingerprinting Kloeker S., Wadzinski BE. Soluble proteins from bovine testis were incubated with the microcystin- Sepharose. J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation kloeker-1999-2 Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. bovin-testis Bos taurus testis chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Kloeker S., Wadzinski BE. Soluble proteins from bovine testis were incubated with the microcystin- Sepharose. J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation kloeker-1999-3 Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. bovin-testis Bos taurus testis affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Kloeker S., Wadzinski BE. Soluble proteins from bovine testis were incubated with the microcystin- Sepharose. J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation kloeker-1999-4 Purification and identification of a novel subunit of protein serine/threonine phosphatase 4. chlae-cos_6 African green monkey kidney cell line Grivet cos_6 African green monkey kidney cell line affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Kloeker S., Wadzinski BE. Soluble proteins from bovine testis were incubated with the microcystin- Sepharose. J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation pp4c_bovin Serine/threonine-protein phosphatase 4 catalytic subunit PPP4C protein protein bovin Bos taurus (Bovine) Bovine MAEISDLDRQIEQLRRCELIKESEVKALCAKAREILVEESNVQRVDSPVTVCGDIHGQFYDLKELFRVGGDVPETNYLFMGDFVDRGFYSVETFLLLLALKVRYPDRITLIRGNHESRQITQVYGFYDECLRKYGSVTVWRYCTEIFDYLSLSAIIDGKIFCVHGGLSPSIQTLDQIRTIDRKQEVPHDGPMCDLLWSDPEDTTGWGVSPRGAGYLFGSDVVAQFNAANDIDMICRAHQLVMEGYKWHFNETVLTVWSAPNYCYRCGNVAAILELDEHLQKDFIIFEAAPQETRGIPSKKPVADYFL f1mrs1_bovin PPP4R1 protein protein bovin Bos taurus (Bovine) Bovine MLTPLGRLDKYAESENVFNRQMVARSLLDTLREVCDDERECIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPAIPYAFSKYLLPIVVRYLADQNNQVRKTSQAALLALLEQELIERVDVETKVCPVLVELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEENKSSEDTSVEDKNSEKIRGADAPEDIEARPTGGDSRVHLEETLAEGAETLRQPPGELSAPAESSSPLRLSPEPPPDAAGSEEEQEPCPCGLASQPEAGTGAQDSAPLDQELYNSFHFWRTPLPEIDLDVELQQGSGSEPGPQGLDVEPEVPAPGSASITMATRKELEEMIENLEPHIDDPDVKAQVDVLAAALRASSLDAGDEAGEAPRRAPQDELPSCRLDHDAAGPLISEETMDSALHYIHNDSDSGTSSGFSPDEERRAKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVRKLHRAAPPTFGADLIQELVESFGRCPRWSGRQAFVFVCQTVIEDDCLPMDQFAVQLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKEHFLTSASCHQEAVEQTIMALQMDRDSDVKYFASTHPASTRISEDALSTASSTY pp4c_cerae_protein Serine/threonine-protein phosphatase 4 catalytic subunit protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P97470 pp4r1_human Serine/threonine-protein phosphatase 4 regulatory subunit 1 PPP4R1 MEG1 PP4R1 protein protein human Homo sapiens Human MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY ppp4r1-ppp4c-1 Copurification of PPP4R1 and PPP4C 896400 n/a 896404 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 n/a 896405 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 physical association physical association false false false Fig. 3 Stoichiometry estimated from intensity of silver staining of the protein separation gel. ppp4r1-ppp4c-2 Copurification of PPP4R1 and PPP4C 896401 n/a 896404 unspecified role unspecified role neutral component neutral component n/a 896405 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 6B ppp4r1-ppp4c-3 Copurification of PPP4R1 and PPP4C 896402 n/a 896404 unspecified role unspecified role neutral component neutral component n/a 896405 unspecified role unspecified role neutral component neutral component physical association physical association false false false Fig. 7 ppp4r1-ppp4c-4 Interaction between PPP4C and PPP4R1 demonstrated by coimmunoprecipitation 896403 n/a 896406 unspecified role unspecified role prey prey n/a 896407 unspecified role unspecified role bait bait n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false