IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ho-2009-2 CHL1 functions as a nitrate sensor in plants. arath-mesophyll Arabidopsis thaliana mesophyll mesophyll Mesophyll of plant leaf The photosynthetic parenchyma between the epidermal layers of a foliage leaf. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Ho CH., Lin SH., Hu HC., Tsay YF. Mesophyll protoplasts were isolated as described by Waadt et al., 2008. yftsay@gate.sinica.edu.tw Cell (0092-8674) 2009 Only protein-protein interactions 3 imex curation ho-2009-1 CHL1 functions as a nitrate sensor in plants. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Ho CH., Lin SH., Hu HC., Tsay YF. yftsay@gate.sinica.edu.tw Cell (0092-8674) 2009 Only protein-protein interactions 3 imex curation cipkn_arath CBL-interacting serine/threonine-protein kinase 23 LKS1 PKS17 SnRK3.23 SOS2-like protein kinase PKS17 SNF1-related kinase 3.23 Protein LOW-K(+)-SENSITIVE 1 F12P21.6 CIPK23 At1g30270 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASRTTPSRSTPSRSTPSGSSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDKISSNGRLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVREDGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKIFKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDDVDAIFDDSGESKNLVVERREEGLKTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRKTRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEEGTDGGGTNGAMANRTIAKQST ptr7_arath Protein NRT1/ PTR FAMILY 6.3 NPF6.3 CHL1 NRT1 NRT1.1 Nitrate transporter 1.1 Nitrate/chlorate transporter Protein CHLORINA 1 F12F1.1 T28K15_13 At1g12110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSLPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDDEPSIPMGH cipk23-chl1-2 Colocalisation of CIPK23 and CHL1 shown by BiFC 859507 n/a 859509 unspecified role unspecified role neutral component neutral component n-terminal yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 859510 unspecified role unspecified role neutral component neutral component c-terminal yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5C, Supp Fig 7 cipk23-chl1-3 Colocalisation of CIPK23 and CHL1 shown by BiFC 859507 n/a 859510 unspecified role unspecified role neutral component neutral component n-terminal yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 859509 unspecified role unspecified role neutral component neutral component c-terminal yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5C, Supp Fig 7 cipk23-chl-1 859508 n/a 859510 unspecified role unspecified role bait bait region c-terminal ubiquitin tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region n-terminal transmembrane region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range false n/a 859509 unspecified role unspecified role prey prey region N-terminal ubiquitin tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 5A, 5B The coordinates of the N-terminal transmembrane region are not given in the text so have been inferred from the UniProtKB entry.