MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint retzlaff-2009-2 Hsp90 is regulated by a switch point in the C-terminal domain. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. EMBO Rep. (1469-221X) 2009 Only protein-protein interactions imex curation retzlaff-2009-1 Hsp90 is regulated by a switch point in the C-terminal domain. in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Retzlaff M., Stahl M., Eberl HC., Lagleder S., Beck J., Kessler H., Buchner J. EMBO Rep. (1469-221X) 2009 Only protein-protein interactions imex curation hsp82_yeast ATP-dependent molecular chaperone HSP82 HSP90 YPL240C HSP82 Heat shock protein Hsp90 heat-inducible isoform 82 kDa heat shock protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVPADTEMEEVD D7C35676D668FB63 hsp82-1 859704 hsp82_yeast Hsp90 859706 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified Stoichiometry: 2.0 direct interaction direct interaction false false false 859704 t1 mint hsp82-1 859704 hsp82_yeast Hsp90 859706 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P02829:p.Ala577Ile mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 2.0 direct interaction direct interaction false false false 859704 t1 mint hsp82-1 859704 hsp82_yeast Hsp90 859706 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified P02829:p.Ala577Asn mutation increasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 859704 t1 mint hsp82-hsp82 859705 hsp82_yeast Hsp90 859706 predetermined predetermined participant predetermined unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept purified purified fusion proteins fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false hsp82_yeast HSP90 859706 predetermined predetermined participant predetermined unspecified role unspecified role fluorescence donor fluorescence donor purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false mint f3a st2