IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yang-2009a-2 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-1 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-3 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-4 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-5 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation q4vhg6_9tryp TRF protein protein 9tryp Trypanosoma brucei MYCHAGVPSTSPSPTDFSEVHKILCGHIKQGNNFSVAAAVALEPLHGSSEFWRTCGYRSGIVSALLASSLGVQRETDQLSHLEPMTVADVYAMFRHVVDENAHLRQIIARLESGECDPDDGSGGITSSAERLRSLISEGYDALTGRSSPCMGEDDCGQTPGKWKTCSSGAAGTKRRRDSPEVTERAYVRPESPVPASETLSPAANSGDDTVDEAEQNNALDVLEQLPALPGTTRTVSSFDAVGEMVNRGAGGPSLHPTNPSEFRVGSERGSTIETHSSSELSNFANVGVGTSSGKQKRYKFSASEDEAIIKGLARFTKGQQRFQQIYYAYRSVWHPARTVSQLYDHWRGTLRYKVIQQQGYRGKNSVAARSPEKASNMENNE q387l4_tryb2 Tb11.03.0760 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTDVDGGSGASWKRLRFFVCADTLLTFQPVDSSHLEGRPGQPEQRYLLVDGEYRPITSYPTSSLFLETLKLAGSTVVVIAPWKRAKTKKLLSLFGWTAFDEIRSIHPEPGRDKLHLACIGCSADEYSTSVIVATSRADWSPSMYPQLVEVKHGVNQVPHSLALLRALSSCLRLAEWQVRLPHVPISSCATLCRSRLFSHCRFFLEPVVSEDATVALSINEHGGYIVTRREDATHCVVSGSLPNLPGDISNLPPGGCAAKAVKECSVLPTSAEGRSAEPKSAKVDTDSVSLVKNEVDESDDSIKDEFSYSAGNAATAMVECGDEGVWNEENADDEDPVTMIPPGTFSEDDEDDAPGGLEEADGETVTTVTTQWLKDCAEGLFMYPPQVSHTSKDGSDWNITGLLLATILPTSDAEPLLLSSFNTFAERSGFEPLQLNSADEGGLAEVMQRQWGGVLCMEEKDGDFLLSQTNIYTEAFSDEMDKHLTFVANRFCEAWKDAVQRRRALLGSDAGTQTTVSITVTFGTNTEVEVAPSVQPPPPPPPAPQPTTASAAANEGSKESATPALPFLMRALGPAEWAKEVEAPRSPPPSAVPDIPLKQDPVPSGEGVATAENSVGNDQKESTSRQVAGDAGDTANVAEATEEIAALDQPFEKCFIPTEALGSDREGLDRTQLERQLPFRNYPIKLNVSKSGIFCQFPTVSDAKRFYEEGTVEILNRSLPIKPVFEKRNETVAPAERKRRRSVSPGGVHPQTAAVSALSRRGIQGATYARTQRSRTDNGWKAEGDRNPFVLTEEENSLLTSLGITYTYALENVPLLEHYAQKDLSSKVYRKEKGVLVKMLEKLKAVKGTTSKAPQ trb-rap1-2 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by Y2H 814498 n/a 814503 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814504 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 814509 814507 physical association physical association false false false Fig. 2A trb-trb-1 Homodimerisation of Trb by Y2H 814498 n/a 814503 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814503 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 814514 814512 physical association physical association false false false Fig. 2A trb-rap1-1 814499 n/a 814504 unspecified role unspecified role prey prey n/a 814503 unspecified role unspecified role bait bait physical association physical association false false false trb-rap1-3 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by coimmunopreciptiation 814500 n/a 814504 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814503 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2B trb-rap1-4 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by coimmunopreciptiation 814501 n/a 814503 unspecified role unspecified role bait bait n/a 814504 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B trb-rap1-5 Colocalisation of Trb and Rap1 (Tb11.03.0760) 814502 n/a 814503 unspecified role unspecified role neutral component neutral component n/a 814504 unspecified role unspecified role neutral component neutral component ty1 region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 2C