MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint lee-2009e-1 Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation lee-2009e-2 Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation lee-2009e-3 Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lee CY., Stewart EV., Hughes BT., Espenshade PJ. EMBO J. (0261-4189) 2009 2009-03-09 Only protein-protein interactions imex curation ofd1_schpo Prolyl 3,4-dihydroxylase ofd1 PKHD-type hydroxylase ofd1 ofd1 2-oxoglutarate and Fe(II) dioxygenase domain-containing protein 1 uS12 prolyl 3,4-dihydroxylase SPBC6B1.08c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGDTQQPTVLDRFAPHALDTSEAERLSREYHKNGPYNHVVIRPLINDTLLRNVRKELMENIHFTEKVTDIYRVWQTGDLANLDGLDKKEQNMLKYLRQVRDALYSEEFRSHVQKITGCGPLSASKKDLSVNVYSKGCHLMNHDDVIGTRCISYILYLVEPDEGWKPEYGGALRLFPTLQPSFPAADFCHSIPPQWNQLSFFRVKPGHSFHDVEEVYVDKPRMAISGWFHYPQPGEPGYDANYCDNTVSTLQSLTMKQMDLELPRFSYYPVVKPEPLSKQDTDILSNYINPLYLTPDGIEKLSKRFFQDSVIVLVEFLNQEFANTLLKRIIDAERQPTPMHSSEVSFPWKTAIPPHKHRYLYLDHEEFGPDIILPMDLQRLPAFQRWIQLVSGLPLRSFHQVGRRFRPGSDFTLATTNDTALLEATLCLSPGTGIANTDNGAYDIYMIGDEDPDVDAAVYRGDQQDDSILLSLPAAWNVFSLVYRDEGVLQFVKYVSRQAESSRWDIYSQWNPVAE 4A6EBD870424EE0B mint ett1_schpo Negative regulator of ofd1 SPCC4B3.07 nro1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MIGRRPQGLRAAASLKKQQQLEKQKQEASYELSGNSSPSKENGSENVDNGEMEDETMLVYTEEDNISQLWGLYEMSREKLENDDIDASVSLVFGTIHEADRILRNTEDISTLPKDFHAAYSSALLAVSELFEIAQKRLKETNTEESYIDAAIERAQLGLDAPGNESRLFLALARAYLEKVRVLVWRHDNEESLANIPVTQLVNPYIEKAIQYLRPLAQDSTEYFDALTPDSLRPLYILSSYLFQFGDQFSEAFLLDVCSIITALWLKSVVDPNTPAYYKLIAQEAVLNNYTTFAEYYMDLLDNSESNVDDLINKASSWLNNSVDTWNVIYTLDKSPERLLKLADIKMDLAQIVQDEASQDNYLKEACNAIKEAQGSGVELSPDYVEFVEAYSA 081D5A027088DDDC mint ofd1-nro1 800641 ofd1_schpo ofd1 800644 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F5B F7B F7C mint nro1-ofd1 800641 yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level ofd1_schpo ofd1 800644 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell binding site binding region binding-associated region binding region certain certain sequence position certain c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false physical association physical association false false false F5D mint ofd1-nro1-1 800641 ofd1_schpo ofd1 800644 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.His210Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F6B mint ofd1-nro1-2 800641 ofd1_schpo ofd1 800644 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.His142Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F6B mint ofd1-nro1-3 800641 ofd1_schpo ofd1 800644 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell Q11120:p.Asp144Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q11120:p.His142Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F6B mint nro1-ofd1-1 800642 yj27_schpo Nro1 800645 anti tag immunost anti tag immunostaining anti tag immunost unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell myc tagged myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ofd1_schpo ofd1 800644 immunostaining immunostaining unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false F5A mint ofd1-nro1-4 800643 yj27_schpo Nro1 800645 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified his tagged his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ofd1_schpo ofd1 800644 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false F5C mint