IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ stitzel-2007-2 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Stitzel ML., Cheng KC., Seydoux G. Tagged proteins were immunostained with an anti-tag antibody. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation stitzel-2007-3 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. caeel Caenorhabditis elegans anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stitzel ML., Cheng KC., Seydoux G. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation stitzel-2007-5 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Stitzel ML., Cheng KC., Seydoux G. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation stitzel-2007-1 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. caeel-oocyte Caenorhabditis elegans oocyte oocyte Oocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Stitzel ML., Cheng KC., Seydoux G. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation stitzel-2007-4 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Stitzel ML., Cheng KC., Seydoux G. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation stitzel-2007-6 Regulation of MBK-2/Dyrk kinase by dynamic cortical anchoring during the oocyte-to-zygote transition. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Stitzel ML., Cheng KC., Seydoux G. gseydoux@jhmi.edu Curr. Biol. (0960-9822) 2007 Only protein-protein interactions imex curation mbk2_caeel Dual specificity tyrosine-phosphorylation-regulated kinase mbk-2 mbk-2 F49E11.1 Minibrain Kinase 2 Dual specificity Yak1-related kinase mbk-2 protein protein caeel Caenorhabditis elegans MAALASFTRNSRSYGQQPIDVTQQGQRDRSVMSLDAQGRSVSHECPTSTTLVRQLYLPQIPQSASFAAAPTSFSGASSSSSNHHHPVYHSQNSLPPNLLGSSQNSASSNSLVQGHRNPALGSGNTLTRSYHQPSSTNSSTNNLYGPLGTISRDLKQSIRDISPPVINSSANPHLVNYVQTSSFDNGSYEFPSGQAQQQRRLGGSQQHLAPLQQTASSLYSNPQSSSSQLLGQQQAVRPNYAYQQSLPRQQHINSHQTQAFFGTVRGPTNSTNIVTPLRASKTMIDVLAPVRDTVAAQATGLPNVGTSSSNGSSNSSSGVGSGGSGSLMTQSIGGPNKHLSASHSTLNTASTHDMMHSKIPKSPSNESLSRSHTSSSGGSQGGHNSNSGSNSGFRPEDAVQTFGAKLVPFEKNEIYNYTRVFFVGSHAKKQAGVIGGANNGGYDDENGSYQLVVHDHIAYRYEVLKVIGKGSFGQVIKAFDHKYQQYVALKLVRNEKRFHRQADEEIRILDHLRRQDSDGTHNIIHMLDYFNFRNHKCITFELLSINLYELIKRNKFQGFSLMLVRKFAYSMLLCLDLLQKNRLIHCDLKPENVLLKQQGRSGIKVIDFGSSCFDDQRIYTYIQSRFYRAPEVILGTKYGMPIDMWSLGCILAELLTGYPLLPGEDENDQLALIIELLGMPPPKSLETAKRARTFITSKGYPRYCTATSMPDGSVVLAGARSKRGKMRGPPASRSWSTALKNMGDELFVDFLKRCLDWDPETRMTPAQALKHKWLRRRLPNPPRDGLESMGGLADHEKKTETLPNIDSNANILMRKKF egg3_caeel Protein tyrosine phosphatase-like protein egg-3 egg-3 Egg sterile protein 3 F44F4.2 protein protein caeel Caenorhabditis elegans MRTSDSHLPLSNLARSDSIEFKDAVINEKWVHSANRRKGHLTPKAPEKKSGWFGGQKSEEELLMERVEQHELEELQTFIAQKLFRVDGIICDEETRILLVEKLMNAKEYPTINHDELAHRYGSSMAGWLRDRLVPSMSDCSSVLQRAAAEFYQNKMSDPLCNWGQLNPEHVSMVAARIAKFSEEMSSKVKWSLLVEPGKFSCHLTEFVQEFNRLDRMFVSNELSDEESLQTAFNANYLTKARSKMVPCAEFSRVKLNDGLGRLDDRNELRNGMFSDEHEFLQEEGYTAKSTYGTTDFIHANYVKGGPLLNTFICAQAPLKNTQEDFWRMVFQEKCQFIVMLNSAVDSSTLGPLDSANRNHCPYYWPRAENESLRFGSFHITCMKVDSKADPLFTITKLKVQKVGGNLLDAEFDEELFLEHWQWDWQYLGDVHWPFRVLRKARQLSTPTIVQCIDGCSKSGTLVSIETALMHFIRGSPITKSLILQSCVFVRLQRRLSVSSVLLYLFIYRVILRWIEPYVNKWYHRAALGLRFKSIGFIQKYNAMIQEFSRITPAY dyrktide_peptide Dyrktide peptide protein protein chemical synthesis Chemical synthesis (Chemical synthesis) RRRFRPASPLRGPPK An amino acid sequence which is not represented in UniProt mbk2-egg3-2 Colocalisation of MBK2 and EGG3 862898 n/a 862904 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 862905 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2B, 2C MBK-2 colocalizes with endogenous EGG-3 on the cortex in metaphase I embryos and in discrete cytoplasmic puncta during the second meiotic division. mbk2-egg3-3 Colocalisation of MBK2 and EGG3 862898 n/a 862904 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 862905 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Supp. Fig. 1C MBK-2 colocalizes with endogenous EGG-3 on the cortex in metaphase I embryos and in discrete cytoplasmic puncta during the second meiotic division. mbk2-egg3-4 Interaction between MBK2 and EGG3 demonstrated by coimmunoprecipitation 862899 n/a 862904 unspecified role unspecified role prey prey n/a 862905 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3A mbk2-egg3-5 Interaction between MBK2 and EGG3 demonstrated by coimmunoprecipitation 862899 n/a 862904 unspecified role unspecified role bait bait n/a 862905 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3B mbk2-egg3-7 Interaction between MBK2 and EGG3 demonstrated by coimmunoprecipitation 862900 n/a 862905 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 862904 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3D mbk2-egg3-8 Interaction between MBK2 and EGG3 demonstrated by coimmunoprecipitation 862900 n/a 862905 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) n/a 862904 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 3D mbk2-egg3-1 Colocalisation of MBK2 and EGG3 862901 n/a 862904 unspecified role unspecified role neutral component neutral component n/a 862905 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false Fig. 2A mbk2-egg3-6 Interaction between MBK2 and EGG3 demonstrated by coimmunoprecipitation 862902 n/a 862904 unspecified role unspecified role prey prey n/a 862905 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3C mbk2-dyrktide-1 Protein kinase activity of MBK2 862903 n/a 862904 enzyme enzyme neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q9XTF3:p.Lys490Arg Alters kinetics of interaction mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false caeel Caenorhabditis elegans n/a 862906 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation false false false Fig. 5H Phosphorylation of Dyrktide