IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ quan-2007-2 SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. arath-mesophyll Arabidopsis thaliana mesophyll mesophyll Mesophyll of plant leaf The photosynthetic parenchyma between the epidermal layers of a foliage leaf. bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Quan R., Lin H., Mendoza I., Zhang Y., Cao W., Yang Y., Shang M., Chen S., Pardo JM., Guo Y. Mesophyll protoplasts were prepared and transfected using Arabidopsis leaves as described in Sheen, 2001. guoyan@nibs.ac.cn Plant Cell (1040-4651) 2007 Only protein-protein interactions imex curation quan-2007-1 SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Quan R., Lin H., Mendoza I., Zhang Y., Cao W., Yang Y., Shang M., Chen S., Pardo JM., Guo Y. guoyan@nibs.ac.cn Plant Cell (1040-4651) 2007 Only protein-protein interactions imex curation quan-2007-3 SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay autoradiography autoradiography Quan R., Lin H., Mendoza I., Zhang Y., Cao W., Yang Y., Shang M., Chen S., Pardo JM., Guo Y. guoyan@nibs.ac.cn Plant Cell (1040-4651) 2007 Only protein-protein interactions imex curation quan-2007-4 SCABP8/CBL10, a putative calcium sensor, interacts with the protein kinase SOS2 to protect Arabidopsis shoots from salt stress. in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Quan R., Lin H., Mendoza I., Zhang Y., Cao W., Yang Y., Shang M., Chen S., Pardo JM., Guo Y. guoyan@nibs.ac.cn Plant Cell (1040-4651) 2007 Only protein-protein interactions imex curation cnbla_arath Calcineurin B-like protein 10 SOS3-like calcium binding protein 8 F26P21_120 At4g33000 CBL10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTTGRPNNILALKISTRSSSLTVGEQFCAVFIPFFAIIDVLVSSVGQCFDCRSTSPRTCQHADLERLARESQFSVNEVEALYELFKKLSCSIIDDGLIHKEELRLALFQAPYGENLFLDRVFDLFDEKKNGVIEFEEFIHALSVFHPYASIQEKTDFAFRLYDLRQTGFIEREEVQQMVSAILLESDMMLSDELLTMIIDKTFADADSDKDGKISKDEWNVYVHKHPSLLKNMTLPYLKDVTTAFPSFIFNTEVED cipko_arath CBL-interacting serine/threonine-protein kinase 24 SNF1-related kinase 3.11 Protein SALT OVERLY SENSITIVE 2 CIPK24 SnRK3.11 SOS2 At5g35410 F6I13.1 K21B8.3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTKKMRRVGKYEVGRTIGEGTFAKVKFARNTDTGDNVAIKIMAKSTILKNRMVDQIKREISIMKIVRHPNIVRLYEVLASPSKIYIVLEFVTGGELFDRIVHKGRLEESESRKYFQQLVDAVAHCHCKGVYHRDLKPENLLLDTNGNLKVSDFGLSALPQEGVELLRTTCGTPNYVAPEVLSGQGYDGSAADIWSCGVILFVILAGYLPFSETDLPGLYRKINAAEFSCPPWFSAEVKFLIHRILDPNPKTRIQIQGIKKDPWFRLNYVPIRAREEEEVNLDDIRAVFDGIEGSYVAENVERNDEGPLMMNAFEMITLSQGLNLSALFDRRQDFVKRQTRFVSRREPSEIIANIEAVANSMGFKSHTRNFKTRLEGLSSIKAGQLAVVIEIYEVAPSLFMVDVRKAAGETLEYHKFYKKLCSKLENIIWRATEGIPKSEILRTITF cnbl4_arath Calcineurin B-like protein 4 Protein SALT OVERLY SENSITIVE 3 CBL4 SOS3 At5g24270 MOP9.8 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCSVSKKKKKNAMRPPGYEDPELLASVTPFTVEEVEALYELFKKLSSSIIDDGLIHKEEFQLALFRNRNRRNLFADRIFDVFDVKRNGVIEFGEFVRSLGVFHPSAPVHEKVKFAFKLYDLRQTGFIEREELKEMVVALLHESELVLSEDMIEVMVDKAFVQADRKNDGKIDIDEWKDFVSLNPSLIKNMTLPYLKDINRTFPSFVSSCEEEEMELQNVSS calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p3-arath-pep Recognition sequences of protein kinase C or SNF1/AMPK protein protein chemical synthesis Chemical synthesis (Chemical synthesis) ALARAASAAALARRR An amino acid sequence which is not represented in UniProt sos2-scabp8-5 830436 n/a 830440 unspecified role unspecified role neutral component neutral component region C-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 830441 unspecified role unspecified role neutral component neutral component region N-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range false direct interaction direct interaction false false false Figure 5B sos2-sos3 830436 n/a 830442 unspecified role unspecified role neutral component neutral component region C-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 830441 unspecified role unspecified role neutral component neutral component region N-terminal region of YFP protein yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false Figure 5B sos2-scabp8-4 830437 n/a 830441 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind range range range range false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 830440 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 5A The authors do not provide the amino acid coordinates of the FISL domain deleted from the SOS2 protein. The region is represented by coordinates obtained from the UniProt record for the protein and shown as fuzzy coordiantes to indicate the coordinates of the deleted region are not explicitly stated in the text. scabp8-calcium 830438 n/a 830443 unspecified role unspecified role prey prey n/a 830440 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Figure 7E Calcium is radio-labelled. sos2-pep3 830439 n/a 830444 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 830441 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Figure 7F