IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ van_der_bie-2000-1 Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence van der Biezen EA., Sun J., Coleman MJ., Bibb MJ., Jones JD. jonathan.jones@bbsrc.ac.uk. jonathan.jones@bbsrc.ac.uk Arabidopsis cDNA library made from mRNA isolated from a number of sources. These were healthy leaves of 4-week-old wild-type Arabidopsis plants and pad4 mutant plants (Note: PAD4 gene was previously shown to be required for synthesis of camalexin in response to infection by the virulent bacterial pathogen) and from leaves of these plants harvested at several time points after infection with Pseudomonas syringae pv. tomato carrying AvrRPS4 or P. parasitica Noco2. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 Only protein-protein interactions imex curation o04264_arath RPP5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAASSSSGRRRYDVFPSFSGVDVRKTFLSHLLKALDGKSINTFIDHGIERSRTIAPELISAIREARISIVIFSKNYASSTWCLNELVEIHKCFNDLGQMVIPVFYDVDPSEVRKQTGEFGKVFEKTCEVSKDKQPGDQKQRWVQALTDIANIAGEDLLNGPNEAHMVEKISNDVSNKLITRSKCFDDFVGIEAHIEAIKSVLCLESKEARMVGIWGQSGIGKSTIGRALFSQLSSQFHHRAFLTYKSTSGSDVSGMKLSWQKELLSEILGQKDIKIEHFGVVEQRLNHKKVLILLDDVDNLEFLKTLVGKAEWFGSGSRIIVITQDRQLLKAHEIDLVYEVKLPSQGLALKMISQYAFGKDSPPDDFKELAFEVAELVGSLPLGLSVLGSSLKGRDKDEWVKMMPRLRNDSDDKIEETLRVGYDRLNKKNRELFKCIACFFNGFKVSNVKELLEDDVGLTMLAEESLIRITPVGYIEMHNLLEKLGREIDRAKSKGNPGKRQFLTNFEDIREVLTEKTGTETLLGIRLPHPGYLTTRSFLIDEKSFKGMRNLQYLEIGYWSDGVLPQSLVYFPRKLKRLWWDNCPLKRLPSNFKAEYLVELRMVNSKLEKLWDGTQPLGSLKKMDLYNSYKLKEIPDLSLAINLEELNLEECESLETLPSSIQNAIKLRELNCWGGLLIDLKSLEGMCNLEYLSVPSWSSRECTQGIVYFPRKLKSVLWTNCPLKRLPSNFKAEYLVELIMEYSELEKLWDGTQSLGSLKEMNLRYSNNLKEIPDLSLAINLEELDLFGCVSLVTLPSSIQNATKLIYLDMSECENLESFPTVFNLKSLEYLDLTGCPNLRNFPAIKMGCAWTRLSRTRLFPEGRNEIVVEDCFWNKNLPAGLDYLDCLMRCMPCEFRSEQLTFLNVSGCKLEKLWEGIQSLGSLEEMDLSESENLKELPDLSKATNLKLLCLSGCKSLVTLPSTIGNLQNLRRLYMNRCTGLEVLPTDVNLSSLETLDLSGCSSLRTFPLISTNIVCLYLENTAIEEIPDLSKATKLESLILNNCKSLVTLPSTIGNLQNLRRLYMNRCTGLELLPTDVNLSSLETLDLSGCSSLRTFPLISTRIECLYLENTAIEEVPCCIEDFTRLTVLRMYCCQRLKNISPNIFRLTSLTLADFTDCRGVIKALSDATVVATMEDHVSCVPLSENIEYTCERFWDACSDYYSDDFEVNRNPIRLSTMTVNDVEFKFCCSITIKECGVRLLYVYQETEHNQQTTRSKKRMRMTSGTSEEDINLPYGQIVADTGLAALNTELSLGQGEASSSTSLEGEALCVDDYMINEEQDEQIPILYPVYDIDDDMWRSLFFGDTDDDMWRSLYSAE q9m5p7_arath RSH1 At4g02260 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSASSMSVSVECVNICNLTKGDGNARSDCSALSCAWKAPRALTGFLASTAHPPVCSVYSCGRNGRKSRMKACAWQRYEYEVGFSEAPYFVNVRNILKSRLSCGGHKRWELYCVSAESSSGASSDVTVETLWEDLFPSISYLPRKELEFVQKGLKLAFEAHHGQKRRSGEPFIIHPVAVARILGELELDWESIVAGLLHDTVEDTNFITFEKIEEEFGATVRHIVEGETKVSKLGKLKCKTESETIQDVKADDLRQMFLAMTDEVRVIIVKLADRLHNMRTLCHMPPHKQSSIAGETLQVFAPLAKLLGMYSIKSELENLSFMYVSAEDYDRVTSRIANLYKEHEKELTEANRILVKKIEDDQFLDLVTVNTDVRSVCKETYSIYKAALKSKGSINDYNQIAQLRIVVKPKPSVGVGPLCSPQQICYHVLGLVHEIWKPIPRTVKDYIATPKPNGYQSLHTTVIPFLYESMFRLEVQIRTEEMDLIAERGIAVYYNGKSLSTGLVGNAVPLGRNSRGKTGCLNNADFALRVGWLNAIREWQEEFVGNMSSREFVDTITRDLLGSRVFVFTPKGEIKNLPKGATVVDYAYLIHTEIGNKMVAAKVNGNLVSPTHVLENAEVVEIVTYNALSSKSAFQRHKQWLQHAKTRSARHKIMRFLREQAAQCAAEITQDQVNDFVADSDSDVEDLTEDSRKSLQWWEKILVNVKQFQSQDKSRDTTPAPQNGSVWAPKVNGKHNKAIKNSSSDEPEFLLPGDGIARILPANIPAYKEVLPGLDSWRDSKIATWHHLEGQSIEWLCVVSMDRKGIIAEVTTVLAAEGIALCSCVAEIDRGRGLAVMLFQIEANIESLVSVCAKVDLVLGVLGWSSGCSWPRSTENAQVLEC rpp5-rsh1 RPP5 interacts with RSH1 806615 n/a 806616 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 806617 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 806622 806620 physical association physical association false false false Figure 1a Figure 1b