IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ bischoff-1991-5 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 bischoff-1991-1 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. in vitro In vitro comigration in gel comigration in gel electrophoresis comigration in gel predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 bischoff-1991-2 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. in vitro In vitro chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 bischoff-1991-3 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. in vitro In vitro reverse phase chrom reverse phase chromatography reverse phase chrom predetermined predetermined participant predetermined Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 bischoff-1991-4 Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bischoff FR., Ponstingl H. Soluble fraction of HeLa was subject to ammonium sulphate precipitation followed by complexing with GDP; the complex so formed was purified on Sephacryl S-200. Further purification to homogenity was obtained on hydroxylapatite. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1991 gdp guanosine 5-diphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 rcc1_human Regulator of chromosome condensation Chromosome condensation protein 1 Cell cycle regulatory protein RCC1 CHC1 protein protein human Homo sapiens Human MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFIPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGKAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQDEDAWSPVEMMGKQLENRVVLSVSSGGQHTVLLVKDKEQS ran-gdp-3 756580 N/A 756585 unspecified role unspecified role neutral component neutral component N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction false false false 8 ran-gtp 756580 N/A 756587 unspecified role unspecified role neutral component neutral component N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction false false false 8 ran-rcc1-2 756580 N/A 756588 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 756585 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 8 ran-rcc1 756581 N/A 756588 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction false false false 6B purified RAN and RCC1 proteins used. ran-gdp 756582 N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 756585 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 3 and 4 ran-gdp-1 756583 N/A 756585 unspecified role unspecified role neutral component neutral component N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 direct interaction direct interaction false false false 5 ran-gdp-2 756584 N/A 756586 unspecified role unspecified role neutral component neutral component human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 756585 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 3 and 4