IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ rayapuram-2008-2 The three mitochondrial encoded CcmF proteinsform a complex that interacts with CcmH and C-type apo-cytochromes in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Rayapuram N., Hagenmuller J., Grienenberger JM., Bonnard G., Giege P. Phillippe.Giege@ibmp-ulp.u-strasbg.fr J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation rayapuram-2008-3 The three mitochondrial encoded CcmF proteinsform a complex that interacts with CcmH and C-type apo-cytochromes in Arabidopsis. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rayapuram N., Hagenmuller J., Grienenberger JM., Bonnard G., Giege P. Phillippe.Giege@ibmp-ulp.u-strasbg.fr J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation rayapuram-2008-1 The three mitochondrial encoded CcmF proteinsform a complex that interacts with CcmH and C-type apo-cytochromes in Arabidopsis. arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Rayapuram N., Hagenmuller J., Grienenberger JM., Bonnard G., Giege P. Phillippe.Giege@ibmp-ulp.u-strasbg.fr J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation ccmf2_arath Cytochrome c biogenesis CcmF N-terminal-like mitochondrial protein 2 CCMFN2 CC6BN2 CCB203 Cytochrome c biogenesis orf203 AtMg00960 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress VDTGREQAKRVVRNGKKETTTLPLCWTAGANTVVSDQDQEPIRIWILTCWWFLTVGILLGSWWAYHELGWGGWWFWDPVENASFMPWVLATACIHSVILPFLHSWTLFLNIVTFLCCVLGTFSIRSGLLASVHSFATDDTRGIFLWWFFLLMTGIFMILFYQMKQQASVRRTYKKEMVVARSTLVHLRHSARAQPRPVMLWKN ccmf1_arath Cytochrome c biogenesis CcmF N-terminal-like mitochondrial protein 1 CCMFN1 CC6BN1 CCB382 Cytochrome c biogenesis orf382 AtMg00830 At2g07783 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSISIYEFFHYSLFLGLFVAFTYNKKQPPAFGAALAFWCILLSFLGLLFCHISNNLSNYNVLTANALFFYQISGTWSNHEGSILLWCWILNFYGFFFCYRGRPQSHNVLKQGGYRESLFFFFVLNFVKNSILSLLRYEQESGLKNQLYTPFVLRTLVDSELCSRRNRTFDGPALFYAPLYPERKIKNPLDAWRFRGSREGKRTHPLLHLARDDKERASSIDEQRIDGALGIALFFSFFLLASSDPFVRNFFVCTEPLAELNPVLQDPILAIHPPCIYAGDVASAEGFGLCRSKMMNGIVALHSPPMRKDAAEKNGTLLCSAGCVGSRITSELFTLKFKHVGAKCYPALLLRSNRSPLMLLRRRFFAFSSFWAGARSHSTKRY ccmfc_arath Cytochrome c biogenesis CcmF C-terminal-like mitochondrial protein CCMFC CCB452 CCB6C Cytochrome c biogenesis orf452 AtMg00180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVQLHNFFFFIIFMVVLCGTAAPVLLKWFVSRDVSTGAPFFNGTIIPILISLFSLLVYLHSRKIIRSMDGAKSGVLVRASRPILLPDIIGRSSSETRARKALFFFVPVLHFCLLESKGDFSYLESFCGVLCLLFFCTFFFLARDRSAKRERARRRKGQTLRPNGNEQRRNDKMRCSGHPHLDLERRVEGFGPLAFPVPPELGGACVGGVPPEIGLEALALPRSRQLMAMAVGHDYYQKVPMKMNISHGGVCICMLGVLLSNTKKIQFTQRLPLGYELHMGKERCCLRGLDHLHGPTFHSICGNLMIYKSSLTNDRLMFEHDESLHADLLLINFPASYKNGKLEHFLHWWMKNRKHNNFWLTMFPEKRYFRERTSTAEVAIHTNLFTDLYALIGTGSSRTGGWYTTIMKLPFIFFIWIGFMLASLGGLPSLLRQLQKDKLRWN cyc1_arath Cytochrome c-1 Cytochrome c At1g22840 F19G10.20 At1g22840 CYTC-1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASFDEAPPGNAKAGEKIFRTKCAQCHTVEAGAGHKQGPNLNGLFGRQSGTTAGYSYSAANKNKAVEWEEKALYDYLLNPKKYIPGTKMVFPGLKKPQDRADLIAYLKESTAPK cyc1b_arath Cytochrome c1 2, heme protein, mitochondrial CYC1-2 Complex III subunit 4-2 Complex III subunit IV-2 Cytochrome b-c1 complex subunit 4-2 Ubiquinol-cytochrome c reductase complex cytochrome c1 subunit 2 MHK7.4 At5g40810 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGGGVIRQLLRRKLHSQSVATPVLSWLSSKKANEDAGSAGLRAFALMGAGITGLLSFSTVASADEAEHGLECPNYPWPHEGILSSYDHASIRRGHQVYQQVCASCHSMSLISYRDLVGVAYTEEEAKAMAAEIEVVDGPNDEGEMFTRPGKLSDRLPEPYSNESAARFANGGAYPPDLSLVTKARHNGQNYVFALLTGYRDPPAGISIREGLHYNPYFPGGAIAMPKMLNDEAVEYEDGTPATEAQMGKDVVSFLSWAAEPEMEERKLMGFKWIFLLSLALLQAAYYRRLKWSVLKSRKLVLDVVN ccmh_arath Cytochrome c-type biogenesis CcmH-like mitochondrial protein CCMH F9L1.17 At1g15220 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEKTDEERKKAQMLDARARNISHNVRCTECGSQSIEDSQADIAILLRQLIRNEIGAGKTDKEIYSKLEDEFGETVLYAPKFDLQTAALWLTPVIIAGGTAAGIVYQKHRLRKNVDIMALNLIRGVPLTPKERVTILDVLIPPSPPPQGVVSRLRRWLNR ccmfn1-ccmfn2-1 CCMNFN1 interacts with CCMFN2 760205 N/A 760208 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760209 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A ccmfn1-ccmfn2-2 CCMNFN1 interacts with CCMFN2 760205 N/A 760208 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760209 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A ccmfn2-ccmfn1 CCMFN2 interacts with CCMNFN1 760205 N/A 760208 unspecified role unspecified role bait bait n-terminal CubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760209 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A The authors concluded "CcmFN2 can interact with both CcmFN1 and CcmFC and thus suggest that the 3 mitochondrial encoded Arabidopsis CcmF proteins are assembled in vivo into a protein complex that reconstitute a bacterial-like CcmF". ccmfn2-ccmfn1-1 CCMFN2 interacts with CCMNFN1 760205 N/A 760209 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760208 unspecified role unspecified role bait bait n-terminal CubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 4A The authors concluded "CcmFN2 can interact with both CcmFN1 and CcmFC and thus suggest that the 3 mitochondrial encoded Arabidopsis CcmF proteins are assembled in vivo into a protein complex that reconstitute a bacterial-like CcmF". ccmfn2-ccmfc-1 CCMFN2 interacts with CCMNFC 760205 N/A 760208 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760210 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 4A The authors concluded "CcmFN2 can interact with both CcmFN1 and CcmFC and thus suggest that the 3 mitochondrial encoded Arabidopsis CcmF proteins are assembled in vivo into a protein complex that reconstitute a bacterial-like CcmF". ccmfn2-ccmfc-2 CCMFN2 interacts with CCMNFC 760205 N/A 760208 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760210 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A The authors concluded "CcmFN2 can interact with both CcmFN1 and CcmFC and thus suggest that the 3 mitochondrial encoded Arabidopsis CcmF proteins are assembled in vivo into a protein complex that reconstitute a bacterial-like CcmF". ccmfn2-at1g22840 CCMFN2 interacts with At1g22840 (apo-cytochrome c) 760205 N/A 760211 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760208 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 5 The authors concluded "CcmFN2 can interact with both c-type apo-cytochromes and suggest that in vivo the assembled CcmF indeed interacts with ctype apo-cytocromes at the level of CcmFN2.". ccmfn2-at1g22840-1 CCMFN2 interacts with At1g22840 (apo-cytochrome c) 760205 N/A 760208 unspecified role unspecified role bait bait n-terminal CubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760211 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 5 The authors concluded "CcmFN2 can interact with both c-type apo-cytochromes and suggest that in vivo the assembled CcmF indeed interacts with ctype apo-cytocromes at the level of CcmFN2.". ccmfn2-at5g40810 CCMFN2 interacts with At5g40810 (apo-cytochrome c1) 760205 N/A 760208 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760212 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 5 The authors concluded "CcmFN2 can interact with both c-type apo-cytochromes and suggest that in vivo the assembled CcmF indeed interacts with ctype apo-cytocromes at the level of CcmFN2.". ccmfn2-at5g40810-1 CCMFN2 interacts with At5g40810 (apo-cytochrome c1) 760205 N/A 760212 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760208 unspecified role unspecified role bait bait n-terminal CubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Figure 5 The authors concluded "CcmFN2 can interact with both c-type apo-cytochromes and suggest that in vivo the assembled CcmF indeed interacts with ctype apo-cytocromes at the level of CcmFN2.". at1g15220-ccmfn1-2 At1g15220 (CCMH) interacts with CCMNFN1 760205 N/A 760209 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760213 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 6A at1g15220-ccmfn2-3 At1g15220 (CCMH) interacts with CCMFN2 760205 N/A 760213 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760208 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 6A ccmfc-ccmfn2 CCMNFC interacts with CCMFN2 760205 N/A 760208 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760210 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A ccmfc-ccmfn2-1 CCMNFC interacts with CCMFN2 760205 N/A 760210 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760208 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 4A at5g40810-ccmfn2 At5g40810 (apo-cytochrome c1) interacts with CCMFN2 760205 N/A 760208 unspecified role unspecified role prey prey n-terminal NubPLV tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 760212 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 5 at5g40810-ccmfn2-1 At5g40810 (apo-cytochrome c1) interacts with CCMFN2 760205 N/A 760208 unspecified role unspecified role prey prey c-terminal NubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 760212 unspecified role unspecified role bait bait c-terminal CubPLV tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 5 ccmfn1-at1g15220 CCMNFN1 interacts with At1g15220 (CCMH) 760206 N/A 760213 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 760209 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6B The range for CCMH D1 domain was taken from Meyer et al Proc Natl Acad Sci U S A. 2005 November 1; 102(44): 16113-16118. PMID 16236729. The authors concluded "CCMH can interact with CcmF at the level of both CcmFN1 and CcmFN2 and that these interactions are, at least in part, mediated by two domains localized in the inter-membrane space in CcmFN1 and CcmFN2". ccmfn2-at1g15220 CCMFN2 interacts with At1g15220 (CCMH) 760206 N/A 760208 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 760213 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 6B The authors concluded "CCMH can interact with CcmF at the level of both CcmFN1 and CcmFN2 and that these interactions are, at least in part, mediated by two domains localized in the inter-membrane space in CcmFN1 and CcmFN2". The range for CCMH D1 domain was taken from Meyer et al Proc Natl Acad Sci U S A. 2005 November 1; 102(44): 16113-16118. PMID 16236729. ccmfn1-ccmfn2 Complex of CCMNFN1, CCMFN2 and CCMFC 760207 N/A 760209 unspecified role unspecified role neutral component neutral component N/A 760210 unspecified role unspecified role neutral component neutral component N/A 760208 unspecified role unspecified role neutral component neutral component physical association physical association false false false Figure 3 The complex was judged to be approximately 500kDa in size.