DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lee-2007-1 A protein phosphorylation/dephosphorylation network regulates a plant potassium channel. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lee SC., Lan WZ., Kim BG., Li L., Cheong YH., Pandey GK., Lu G., Buchanan BB., Luan S. sluan@nature.berkeley.edu,view@nature.berkeley.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2007 akt1_arath Potassium channel AKT1 AKT1 At2g26650 F18A8.2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MRGGALLCGQVQDEIEQLSRESSHFSLSTGILPSLGARSNRRVKLRRFVVSPYDHKYRIWEAFLVVLVVYTAWVSPFEFGFLRKPRPPLSITDNIVNAFFAIDIIMTFFVGYLDKSTYLIVDDRKQIAFKYLRSWFLLDLVSTIPSEAAMRISSQSYGLFNMLRLWRLRRVGALFARLEKDRNFNYFWVRCAKLVCVTLFAVHCAACFYYLIAARNSNPAKTWIGANVANFLEESLWMRYVTSMYWSITTLTTVGYGDLHPVNTKEMIFDIFYMLFNLGLTAYLIGNMTNLVVHGTSRTRNFRDTIQAASNFAHRNHLPPRLQDQMLAHLCLKYRTDSEGLQQQETLDALPKAIRSSISHFLFYSLMDKVYLFRGVSNDLLFQLVSEMKAEYFPPKEDVILQNEAPTDFYILVNGTADLVDVDTGTESIVREVKAGDIIGEIGVLCYRPQLFTVRTKRLCQLLRMNRTTFLNIIQANVGDGTIIMNNLLQHLKEMNDPVMTNVLLEIENMLARGKMDLPLNLCFAAIREDDLLLHQLLKRGLDPNESDNNGRTPLHIAASKGTLNCVLLLLEYHADPNCRDAEGSVPLWEAMVEGHEKVVKVLLEHGSTIDAGDVGHFACTAAEQGNLKLLKEIVLHGGDVTRPRATGTSALHTAVCEENIEMVKYLLEQGADVNKQDMHGWTPRDLAEQQGHEDIKALFREKLHERRVHIETSSSVPILKTGIRFLGRFTSEPNIRPASREVSFRIRETRARRKTNNFDNSLFGILANQSVPKNGLATVDEGRTGNPVRVTISCAEKDDIAGKLVLLPGSFKELLELGSNKFGIVATKVMNKDNNAEIDDVDVIRDGDHLIFATDS cipk6_arath CBL-interacting serine/threonine-protein kinase 6 SOS2-like protein kinase PKS4 SNF1-related kinase 3.14 SOS3-interacting protein 3 CIPK6 PKS4 At4g30960 SIP3 SnRK3.14 F6I18.130 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVGAKPVENGSDGGSSTGLLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRMVKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCHSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKKGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKLLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDFLVHKSKEETETLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKDIFWTSTPA cipkn_arath CBL-interacting serine/threonine-protein kinase 23 LKS1 PKS17 SnRK3.23 SOS2-like protein kinase PKS17 SNF1-related kinase 3.23 Protein LOW-K(+)-SENSITIVE 1 F12P21.6 CIPK23 At1g30270 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASRTTPSRSTPSRSTPSGSSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKREISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDKISSNGRLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVREDGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKIFKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDDVDAIFDDSGESKNLVVERREEGLKTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRKTRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEEGTDGGGTNGAMANRTIAKQST cipkg_arath CBL-interacting serine/threonine-protein kinase 16 PKS15 SnRK3.18 SOS2-like protein kinase PKS15 SNF1-related kinase 3.18 F13D4.161 CIPK16 At2g25090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEESNRSSTVLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIEREIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQLISAVDFCHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHTRCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECEFPPWFSLESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPTKKKKKDLNEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKERLETAAREMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTLEYYLFCEDDVRPALKDIVWSWQGDDDEDDVTTNDNVDTNDNKINNVS cnbl1_arath Calcineurin B-like protein 1 SOS3-like calcium-binding protein 5 CBL1 SCABP5 At4g17615 dl4845w FCAALL.122 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSKAAKEFRGHEDPVKLASETAFSVSEVEALFELFKSISSSVVDDGLINKEEFQLALFKSRKRENIFANRIFDMFDVKRKGVIDFGDFVRSLNVFHPNASLEDKIDFTFRLYDMDCTGYIERQEVKQMLIALLCESEMKLADETIEIILDKTFEDADVNQDGKIDKLEWSDFVNKNPSLLKIMTLPYLRDITTTFPSFVFHSEVDEIAT cnbl2_arath Calcineurin B-like protein 2 CBL2 SCABP1 At5g55990 MDA7.3 SOS3-like calcium-binding protein 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCVDGIKHLCTSVLGCFDLDLYKQSGGLGDPELLARDTVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIDDKIHFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLKDTVIEDIIDKTFEEADTKHDGKIDKEEWRSLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT cnbl3_arath Calcineurin B-like protein 3 SOS3-like calcium-binding protein 6 CBL3 SCABP6 At4g26570 T15N24.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSQCIDGFKHVCSSFFRCFDIDIYKQSGGLGDPELLARETVFSVSEIEALYELFKKISSAVIDDGLINKEEFQLALFKTNKKESLFADRVFDLFDTKHNGILGFEEFARALSVFHPNAPIEDKIDFSFQLYDLKQQGFIERQEVKQMVVATLAESGMNLSDEIIESIIDKTFEEADTKHDGRIDKEEWRTLVLRHPSLLKNMTLQYLKDITTTFPSFVFHSQVEDT cnbl9_arath Calcineurin B-like protein 9 CBL9 At5g47100 K14A3.5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCFHSTAAREFPDHENPVKLASETAFSVSEVEALYELFKSISSSVVDDGLINKEEFQLALFKNRKKENLFANRIFDLFDVKRKGVIDFGDFVRSLNVFHPNASLEEKTDFTFRLYDMDCTGFIERQEVKQMLIALLCESEMKLADDTIEMILDQTFEDADVDRDGKIDKTEWSNFVIKNPSLLKIMTLPYLRDITTTFPSFVFNSEVDEIAT p2c03_arath Protein phosphatase 2C 3 AIP1 Protein AKT1-INTERACTING 1 F22G5.22 At1g07430 Protein phosphatase 2C AIP1 HAI2 HON Protein HIGHLY ABA-INDUCED PP2C 2 Protein HONSU protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADICYEDETSACESRPLWSSRKWRIGVQRFRMSPSEMNPTASTTEEEDKSEGIYNKRNKQEEYDFMNCASSSPSQSSPEEESVSLEDSDVSISDGNSSVNDVAVIPSKKTVKETDLRPRYGVASVCGRRRDMEDAVALHPSFVRKQTEFSRTRWHYFGVYDGHGCSHVAARCKERLHELVQEEALSDKKEEWKKMMERSFTRMDKEVVRWGETVMSANCRCELQTPDCDAVGSTAVVSVITPEKIIVANCGDSRAVLCRNGKAVPLSTDHKPDRPDELDRIQEAGGRVIYWDGARVLGVLAMSRAIGDNYLKPYVTSEPEVTVTDRTEEDEFLILATDGLWDVVTNEAACTMVRMCLNRKSGRGRRRGETQTPGRRSEEEGKEEEEKVVGSRKNGKRGEITDKACTEASVLLTKLALAKHSSDNVSVVVIDLRRRRKRHVA akt1-cipk6 Interaction between AKT1 and CIPK6 693650 N/A 693651 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 693652 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1A, 3B, 4B akt1-cipk23 Interaction between AKT1 and CIPK23 693650 N/A 693653 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 693651 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1A, 3A, 4A akt1-cipk16 Interaction between AKT1 and CIPK16 693650 N/A 693654 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 693651 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 1A, 3C, 4C cipk6-cbl1 Interaction between CIPK6 and CBL1 693650 N/A 693652 unspecified role unspecified role bait bait N/A 693655 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B cipk6-cbl2 Interaction between CIPK6 and CBL2 693650 N/A 693652 unspecified role unspecified role bait bait N/A 693656 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B cipk6-cbl3 Interaction between CIPK6 and CBL3 693650 N/A 693657 unspecified role unspecified role prey prey N/A 693652 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk6-cbl9 Interaction between CIPK6 and CBL9 693650 N/A 693658 unspecified role unspecified role prey prey N/A 693652 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk23-cbl3-1 Interaction between CIPK23 and CBL3 693650 N/A 693657 unspecified role unspecified role prey prey N/A 693653 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk23-cbl2-1 Interaction between CIPK23 and CBL2 693650 N/A 693656 unspecified role unspecified role prey prey N/A 693653 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk23-cbl1-2 Interaction between CIPK23 and CBL1 693650 N/A 693655 unspecified role unspecified role prey prey N/A 693653 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk23-cbl9-2 Interaction between CIPK23 and CBL9 693650 N/A 693653 unspecified role unspecified role bait bait N/A 693658 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B cipk16-cbl3-1 Interaction between CIPK16 and CBL3 693650 N/A 693657 unspecified role unspecified role prey prey N/A 693654 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B cipk16-cbl2-1 Interaction between CIPK16 and CBL2 693650 N/A 693654 unspecified role unspecified role bait bait N/A 693656 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B cipk16-cbl1-1 Interaction between CIPK16 and CBL1 693650 N/A 693654 unspecified role unspecified role bait bait N/A 693655 unspecified role unspecified role prey prey physical association physical association false false false Figure 1B cipk16-cbl9-1 Interaction between CIPK16 and CBL9 693650 N/A 693658 unspecified role unspecified role prey prey N/A 693654 unspecified role unspecified role bait bait physical association physical association false false false Figure 1B akt1-at1g07430 Interaction between AKT1 and At1g07430 693650 N/A 693651 unspecified role unspecified role bait bait N/A 693659 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A cipk23-at1g07430-1 Interaction between CIPK23 and At1g07430 693650 N/A 693659 unspecified role unspecified role prey prey N/A 693653 unspecified role unspecified role bait bait physical association physical association false false false Figure 6A