IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sano-2007-4 The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sano H., Ishino M., Kramer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. sanohito@sapmed.ac.jp A rat alveolar macrophage cDNA library was used. J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation sano-2007-1 The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sano H., Ishino M., Kramer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. sanohito@sapmed.ac.jp A rat alveolar macrophage cDNA library was used. J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation sano-2007-2 The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Sano H., Ishino M., Kramer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. The prey protein was identified by mass spectrometry and was shown to be in the lysate using western blotting. sanohito@sapmed.ac.jp A rat alveolar macrophage cDNA library was used. J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation sano-2007-3 The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sano H., Ishino M., Kramer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. sanohito@sapmed.ac.jp A rat alveolar macrophage cDNA library was used. J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation sano-2007-5 The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A. mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sano H., Ishino M., Kramer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y. sanohito@sapmed.ac.jp A rat alveolar macrophage cDNA library was used. J. Biol. Chem. (0021-9258) 2007 Only protein-protein interactions imex curation msre_human Macrophage scavenger receptor types I and II MSR1 SCARA1 Macrophage acetylated LDL receptor I and II Scavenger receptor class A member 1 protein protein human Homo sapiens Human MEQWDHFHNQQEDTDSCSESVKFDARSMTALLPPNPKNSPSLQEKLKSFKAALIALYLLVFAVLIPLIGIVAAQLLKWETKNCSVSSTNANDITQSLTGKGNDSEEEMRFQEVFMEHMSNMEKRIQHILDMEANLMDTEHFQNFSMTTDQRFNDILLQLSTLFSSVQGHGNAIDEISKSLISLNTTLLDLQLNIENLNGKIQENTFKQQEEISKLEERVYNVSAEIMAMKEEQVHLEQEIKGEVKVLNNITNDLRLKDWEHSQTLRNITLIQGPPGPPGEKGDRGPTGESGPRGFPGPIGPPGLKGDRGAIGFPGSRGLPGYAGRPGNSGPKGQKGEKGSGNTLTPFTKVRLVGGSGPHEGRVEILHSGQWGTICDDRWEVRVGQVVCRSLGYPGVQAVHKAAHFGQGTGPIWLNEVFCFGRESSIEECKIRQWGTRACSHSEDAGVTCTL hook3_human Protein Hook homolog 3 HOOK3 protein protein human Homo sapiens Human MFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDPAYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSLEEELRKANAARSQLETYKRQVVELQNRLSEESKKADKLDFEYKRLKEKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSSRRSYPGHVQPATAR q7tq77_rat protein protein rat Rattus norvegicus (Rat) Rat MERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFSFKIRNSCLKLVIEGARMARTVKELAAKPDSLSSLPEPARAHTHMHSHTHTLTYMHTGKDSVLGPMGNYRPRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSLEEELRKANAARGQLETYKRQVVELQNRLSDESKKADKLDFEYKRLKEKVDGLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQDHLSHPSLPFHVYMSQSLASVTPHQYSILIRSPVVALCHGDPVPLEQQDWPFHTSQTIRPVQSPVSGTGWQLNWSALLCCPDEDQGLLSQVLQLVRAGLALPSSHPVGSFLRAGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKINQEGSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKTQRDMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSTRRSYPGHVQPATASLCFFLKFTLQKFHLALVTYKMVLNLNGFSILNSFNNKTKNTRKGTQSLVKLTCAVCQKDADEGAGEVLCWVRACSALAEDLSLVPAPLVRQLIMSSIKHLPEG msre_mouse Macrophage scavenger receptor types I and II Msr1 Scvr Macrophage acetylated LDL receptor I and II Scavenger receptor type A protein protein mouse Mus musculus Mouse MTKEMTENQRLCPHEQEDADCSSESVKFDARSMTASLPHSTKNGPSLQEKLKSFKAALIALYLLVFAVLIPVVGIVTAQLLNWEMKNCLVCSLNTSDTSQGPMEKENTSKVEMRFTIIMEHMKDMEERIESISNSKADLIDTERFQNFSMATDQRLNDILLQLNSLISSVQEHGNSLDAISKSLQSLNMTLLDVQLHTETLNVRVRESTAKQQEDISKLEERVYKVSAEVQSVKEEQAHVEQEVKQEVRVLNNITNDLRLKDWEHSQTLKNITFIQGPPGPQGEKGDRGLTGQTGPPGAPGIRGIPGVKGDRGQIGFPGGRGNPGAPGKPGRSGSPGPKGQKGEKGSVGGSTPLKTVRLVGGSGAHEGRVEIFHQGQWGTICDDRWDIRAGQVVCRSLGYQEVLAVHKRAHFGQGTGPIWLNEVMCFGRESSIENCKINQWGVLSCSHSEDAGVTCTS hook3_mouse Protein Hook homolog 3 Hook3 protein protein mouse Mus musculus Mouse MFNVESVERVELCESLLTWIQTFNVDAPCQTAEDLTNGVVMSQVLQKIDPVYFDDNWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQINDFTLPDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVVMTAIQELMSKESPVSAGHDAYVDLDRQLKKTTEELNEALSAKEEIAQRCHELDMQVAALQEEKSSLLAENQILMERLNQSDSIEDPNSPAGRRHLQLQTQLEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLKDEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQNTVSLEEELRKANAARGQLETYKRQVVELQNRLSDESKKADKLDFEYKRLKEKVDGLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQESSDSLAAEIVTPEIREKLIRLQHENKMLKLNQEDSDNEKIALLQSLLDDANLRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEEHLEKLHEANNELQKKRAIIEDLEPRFNNSSLRIEELQEALRKKEEEMKQMEERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKEYEKTKSQRDMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQATSTRRSYPGHVQPATAR sr_a-hook3-3 Human SR-A coimmunoprecipitates endogenous Hook3 in HEK293 lysates 739384 N/A 739389 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false P21757:p.[Glu12Ala;Asp13Ala;Asp15Ala] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 739390 unspecified role unspecified role prey prey physical association physical association false false false 2 C The negatively charged residues GLu12, Asp13 and Asp15 of SR-A may be essential for binding to Hook3. O60505 transfection sr_a-hook3-4 Human SR-A coimmunoprecipitates endogenous Hook3 in HEK293 lysates 739384 N/A 739389 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 739390 unspecified role unspecified role prey prey c-teminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2 D O60505 transfection sr_a-hook3-5 Human SR-A coimmunoprecipitates endogenous Hook3 in HEK293 lysates 739384 N/A 739390 unspecified role unspecified role prey prey N/A 739389 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 3 O60505 transfection Incubation of cells with fucoidin, an SR-A ligand, increased the levels of this interaction. Incubation of cells with mannose did not singificantly affect the levels of this interaction. sr_a-hook3 Human SR-A interacts with rat Hook3 in a yeast two-hybrid assay 739385 N/A 739389 unspecified role unspecified role bait bait N/A 739391 unspecified role unspecified role prey prey physical association physical association false false false PREY gene AY310154 sr_a-hook3-1 Human SR-A interacts with rat Hook3 in vitro 739386 N/A 739391 unspecified role unspecified role prey prey rat-alveolar_macrophage Rattus norvegicus alveolar macrophage alveolar_macrophage Alveolar macrophage N/A 739389 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false PREY gene AY310154 1 B sr_a-hook3-2 Human SR-A interacts with human Hook3 in vitro 739387 N/A 739389 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 739390 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association false false false 2 B msr1-hook3 Endogenous Msr1 (SR-A) coimmunoprecipitates endogenous Hook3 in mouse macrophage lysate 739388 N/A 739392 unspecified role unspecified role bait bait N/A 739393 unspecified role unspecified role prey prey physical association physical association false false false 4 A