IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kim-2006d-1 SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation kim-2006d-2 SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kim S., Choi K., Park C., Hwang HJ., Lee I. It was not possible to unambiguously identify the protein named SUF3 in this paper. Therefore interactions involving this protein were not curated. ilhalee@snu.ac.kr Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation kim-2006d-3 SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Kim S., Choi K., Park C., Hwang HJ., Lee I. ilhalee@snu.ac.kr Plant Cell (1040-4651) 2006 Only protein-protein interactions imex curation suf4_arath Protein SUPPRESSOR OF FRI 4 SUF4 F17F8.14 At1g30970 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGKKKKRATEKVWCYYCDREFDDEKILVQHQKAKHFKCHVCHKKLSTASGMVIHVLQVHKENVTKVPNAKDGRDSTDIEIYGMQGIPPHVLTAHYGEEEDEPPAKVAKVEIPSAPLGGVVPRPYGMVYPPQQVPGAVPARPMYYPGPPMRHPAPVWQMPPPRPQQWYPQNPALSVPPAAHLGYRPQPLFPVQNMGMTPTPTSAPAIQPSPVTGVTPPGIPTSSPAMPVPQPLFPVVNNSIPSQAPPFSAPLPVGGAQQPSHADALGSADAYPPNNSIPGGTNAHSYASGPNTSGPSIGPPPVIANKAPSNQPNEVYLVWDDEAMSMEERRMSLPKYKVHDETSQMNSINAAIDRRISESRLAGRMAF frigi_arath Protein FRIGIDA F6N23.25 FRI At4g00650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSNYPPTVAAQPTTTANPLLQRHQSEQRRRELPKIVETESTSMDITIGQSKQPQFLKSIDELAAFSVAVETFKRQFDDLQKHIESIENAIDSKLESNGVVLAARNNNFHQPMLSPPRNNVSVETTVTVSQPSQEIVPETSNKPEGGRMCELMCSKGLRKYIYANISDQAKLMEEIPSALKLAKEPAKFVLDCIGKFYLQGRRAFTKESPMSSARQVSLLILESFLLMPDRGKGKVKIESWIKDEAETAAVAWRKRLMTEGGLAAAEKMDARGLLLLVACFGVPSNFRSTDLLDLIRMSGSNEIAGALKRSQFLVPMVSGIVESSIKRGMHIEALEMVYTFGMEDKFSAALVLTSFLKMSKESFERAKRKAQSPLAFKEAATKQLAVLSSVMQCMETHKLDPAKELPGWQIKEQIVSLEKDTLQLDKEMEEKARSLSLMEEAALAKRMYNQQIKRPRLSPMEMPPVTSSSYSPIYRDRSFPSQRDDDQDEISALVSSYLGPSTSFPHRSRRSPEYMVPLPHGGLGRSVYAYEHLAPNSYSPGHGHRLHRQYSPSLVHGQRHPLQYSPPIHGQQQLPYGIQRVYRHSPSEERYLGLSNQRSPRSNSSLDPK lumi_arath Homeobox protein LUMINIDEPENDENS LD T10P11.15 At4g02560 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDAFKEEIEIGSSVESLMELLDSQKVLFHSQIDQLQDVVVAQCKLTGVNPLAQEMAAGALSIKIGKRPRDLLNPKAVKYLQAVFAIKDAISKRESREISALFGITVAQVREFFVTQKTRVRKQVRLSREKVVMSNTHALQDDGVPENNNATNHVEPVPLNSIHPEACSISWGEGETVALIPPEDIPPDISDSDKYFVENIFSLLRKEETFSGQVKLMEWIMQIQDASVLIWFLSKGGVLILTTWLSQAASEEQTSVLLLILKVLCHLPLHKASPENMSAILQSVNGLRFYRISDISNRAKGLLSRWTKLFAKIQAMKKQNRNSSQIDSQSQLLLKQSIAEIMGDSSNPEDILSLSNGKSENVRRIESSQGPKLLLTSADDSTKKHMLGSNPSYNKERRKVQMVEQPGQKAAGKSPQTVRIGTSGRSRPMSADDIQKAKMRALYMQSKNSKKDPLPSAIGDSKIVAPEKPLALHSAKDSPPIQNNEAKTEDTPVLSTVQPVNGFSTIQPVNGPSAVQPVNGPLAVQPVNGPSALQPVNGPSAVIVPVQADEIKKPSTPPKSISSKVGVMMKMSSQTILKNCKRKQIDWHVPPGMELDELWRVAAGGNSKEADVQRNRNRRERETTYQSLQTIPLNPKEPWDREMDYDDSLTPEIPSQQPPEESLTEPQDSLDERRIAAGAATTSSSLSSPEPDLELLAALLKNPDLVYALTSGKPSNLAGQDMVKLLDVIKTGAPNSSSSSNKQVEERVEVSLPSPTPSTNPGMSGWGQEGIRNPFSRQNQVGTAVARSGTQLRVGSMQWHQTNEQSIPRHAPSAYSNSITLAHTEREQQQYMQPKLHHNLHFQQQQQQPISTTSYAVREPVGQMGTGTSSSWRSQQSQNSYYSHQENEIASASQVTSYQGNSQYMSSNPGYESWSPDNSPSRNQLNMRGQQQQASRKHDSSTHPYWNQNKRWR frl1a_arath FRIGIDA-like protein 1 FRL1 Protein SUPPRESSOR OF FRI 8 SUF8 MQK4.4 At5g16320 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTASETIATAINQIDEKKEKLKKAFDDLQAHRSLLSPSFSLSWSEIDSHFSSLQSSLASRFRLLHSTSPLEHDSYRIDASDAGKSSSSEEVSEQPVVEPELRALCEKIDGIGLIKYLIRIWDDETPLNQEVSAAIRYSPDTASMVLDAIEGSNYTPSSSGRSFDVRRVFVLLMEVLIEINANITVDTRNRAKKLAYHWKSKVGVKPFEALVFLHLVAAFELGSEFDTEELSDYVFMIAKYKQATLVCNKIGVDRKRVGKLIKTLLDSGKPILAVKFMYECGMTDEFEPIPVLKSYIKDCREAALRVCVEDNYSLKSQNEASDKEVSALKPLIKIIKDQNLESEFTQEKVEERVEELEKNKALRKRNTTNPPKQEPQQKGKKRTRDCKNGSQVPVPSQQLLSRPEALLMPEHSHHGLQLNPYGLMTSAFSGVVVNPLTGLFGSGATPQSLYYAQQTGYVLPPQYHPPYYSQ flc_arath_gene FLC gene encoding flowering locus C protein gene gene arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress suf4 780994 n/a 780997 unspecified role unspecified role bait bait n/a 780997 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A suf4-fr1 780994 n/a 780997 unspecified role unspecified role bait bait n/a 780998 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A fr1-suf4 780994 n/a 780997 unspecified role unspecified role prey prey n/a 780998 unspecified role unspecified role bait bait physical association physical association false false false Figure 6A fr1-1 780994 n/a 780998 unspecified role unspecified role prey prey n/a 780998 unspecified role unspecified role bait bait physical association physical association false false false Figure 6A ld-suf4 780994 n/a 780999 unspecified role unspecified role bait bait n/a 780997 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A ld-2 780994 n/a 780999 unspecified role unspecified role prey prey n/a 780999 unspecified role unspecified role bait bait physical association physical association false false false Figure 6A frl1-suf4 780994 n/a 780997 unspecified role unspecified role prey prey n/a 781000 unspecified role unspecified role bait bait physical association physical association false false false Figure 6A suf4-frl1-1 780994 n/a 780997 unspecified role unspecified role bait bait n/a 781000 unspecified role unspecified role prey prey physical association physical association false false false Figure 6A suf4-ld 780994 n/a 780999 unspecified role unspecified role prey prey n/a 780997 unspecified role unspecified role bait bait physical association physical association false false false Figure 6B fr1-suf4-1 780995 n/a 780997 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 780998 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6C ld-suf4-1 780995 n/a 780997 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 780999 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6C frl1-suf4-1 780995 n/a 780997 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 781000 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Figure 6C suf4-flc_gene 780996 n/a 780997 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 781001 unspecified role unspecified role prey prey association association false false false Figure 7 The authors state that " a region of FLC promoter ∼545 to 850 bp upstream from the transcription initiation site, detected by the FLC-3 primer set, was highly enriched in 35S-SUF4 tagged with MYC epitope" (immunoprecipitated sample). The authors concluded that this " result showed that SUF4 binds to the region around FLC-3 in vivo, suggesting that SUF4 is recruited to the promoter region of FLC for transcriptional activation".