UniProt UniProt http://www.uniprot.org creutz-2004-1 Characterization of the yeast tricalbins: membrane-bound multi-C2-domain proteins that form complexes involved in membrane trafficking. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Creutz CE., Snyder SL., Schulz TA. creutz@virginia.edu Yeast genomic library prepared in vector pGAD424 Cell. Mol. Life Sci. 61 (1208-1220) 2004 Only protein-protein interactions imex curation tcb1_yeast Tricalbin-1 TCB1 YOR3141c YOR086C protein protein yeast Saccharomyces cerevisiae Baker's yeast MAKEDTGVTAPKKPETAQVANINGIDKLEPPKTKEETESSKSVSSEKAAHASDESFKRSIHEASYVGWKQIGGWEDKDELTLDDELMDMTRETFLDNIIPDSLYGDWYHSVAIFFIGGVASFALGHYKFSMGSAFFVIVITSLLYRTSAKKYRGSIRELVQKEFTVQKVENDYESLEWLNAFLDKYWPILEPSVSQLIVQQANEQMATNEAIPKFITQLWIDELTLGVKPPRVDLVKTFQNTASDVVVMDWGISFTPHDLCDMSAKQVRNYVNELAVVKAKIFGITIPVSVSDIAFKAHARVKFKLMTPFPHVETVNIQLLKVPDFDFVATLFGRSIFNWEILAIPGLMTLIQKMAKKYMGPILLPPFSLQLNIPQLLSGSNLSIGILEITVKNAKGLKRTSSILNESIDPYLSFEFNDISIAKTRTVRDTLNPVWDETLYVLLNSFTDPLTISVYDKRAKLKDKVLGRIQYNLNTLHDKTTQRNLKAQFLRNSKPVGELTFDLRFFPTLEEKKLPDGSVEELPDLNTGIAKVVVEEGSRFAEEEQKVTAYVEVYLNAKLVLTTGKATDTGTLKWNSDYEAVIADRRKTRYKFVVKDGKGEEIGSTIQTLNDLIDRSQVNKNLIPLKNQKGDIKITTYWRPVRLEIGSNSVAYTPPIGAIRVFIEKANDLRNLEKFGTIDPYCKVLVNGLSKGRTDFKSQTLNPVWNQVIYVAVTSPNQRITLQCMDVETVNKDRSLGEFNVNVQDLFKKDENDKYEETIDEKAKVGRLVMPKKKPKGTITYYTSFYPALPVLTLEEIQDLDKVNKKKKALELRKSAIDEKKISKEDKAKFDQEWNEVKELEDMYSNRQKLDLPELLQYNQGVLAVTVLNGELPDSGLYVQAFFDDNGHPRFVSPRIPSRIVKNGWSGDVIIKELDKSITTFRVAKNKNYNRVEKCVCEVELPTQELVKNCYYKPSILHLSGEGSAKLMLQISWFPIDTKQLPANDLITNSGDLTIMSRSAENLIASDLNGYSDPYLKYYINNEEDCAYKTKVVKKTLNPKWNDEGTIQINNRLNDVLRIKVMDWDSTSADDTIGTAEIPLNKVKVEGTTELDVPVEGLENAGQDGGMLHLAFSFKPRYTISVSKREKKVGDIASKGLGTGLKAGTTVIGGGVGAIGKIKKGVFGGLGSLTNHKKNHEMGEEETKF 1CD852549A6D82B1 tcb2_yeast Tricalbin-2 TCB2 N2250 YNL087W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSPNSSKTRTDQISSMPGINEATKVESKNVVKDAVPIKSEVETNGTSIVREKQDPSYVGWKQVGGWEEKDELTSEDLLVDVNKDTFLGNLLPDKFYGDWYHEVAILIIAGLCSFVLGYFKFSLASVLIVMLTTGMLYRTSSKKYRESLRDLAQKEQTVEKITSDYESVEWLNTFLDKYWPIIEPSVSQQIVDGTNTALSENVAIPKFIKAIWLDQFTLGVKPPRIDAIKTFQNTKSDVVVMDVCLSFTPHDMSDLDAKQCRNYVNSNVVLKAKIFGMDIPVSVADIFFQVFVRFRFQLMTTLPLVETINIQLLEVPEVDFISRLLGNSVFNWEILAIPGLMRLIQKMAFKYLSPVLLPPFSLQLNIPQLLSKTGLPIGVLEIKVKNAHGLRKLVGMIKKTVDPYLTFELSGKIVGKTKVFKNSANPVWNESIYILLQSFTDPLTIAVYDKRETLSDKKMGTVIFNLNKLHANHYHKNEKVHFLRNSKPVGELTFDLRFFPTIEPKKLLNGDEEPLPDMNTGITKITIRELKGLDELSDKKFVFAELYVNAELVMTTKKEKRTAHLKWNSDYYSVVTDRRKTICRFVLKDQSGKVISSSVQPLNHLIDRTEVNKEWIPLRNGKGELKVTTYWRPVDIDLGLKSVGYTTPIGMLRVFINKAENLRNPDSLGKISPYAKVSVNGVARGRTNERIETLNPIWNQSIYVSVTSPLQKVSIDCFGIDTNGDDHNLGSLNIQTQNIYHKDNDDKYTIFIDNAPRTGNLIGKKGVKGTVTYYLSFYPVVPVLSLEEAKEVDEINEKKDKLEKQKSTLDDKNISKEEKERIKKEEFRLTEKYDMYSYKMKLDLDELLQYNAGVLGVTVLGGELPQPGLYVQTFFDSCGYAAITSAKNAIRTIKTGWSGDFMIKELEWSVTTFRVTKTKDANKAENFICEVNIPTIELVRNCYYKPSVLNLIGKKSAKLLVQVSWFPVTATELPQSDLITNSGDLKITAKSAENLIGVNKNGYSDPYVEFFLNEKSTSPFFKTAVQKKTLNPTWNESKTIEVSNRVNDYLTINVKDYESTNSNRSIGKAVVPLSTIDPESDTTFNIPLVGPKGEDGGVLHLEFEFEPRYTTNVVKREAGLGNFATKGLGTGIKAGSTVFALGTNVVSTGLGTIDKVKAGVFGGKKSTTTGDKKSEEKQ pdr1_yeast Transcription factor PDR1 PDR1 ANT1 BOR2 CYH3 NRA2 SMR2 AMY1 TIL1 TPE1 TPE3 Pleiotropic drug resistance protein 1 YGL013C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRGLTPKNGVHIETGPDTESSADSSNFSTGFSGKIRKPRSKVSKACDNCRKRKIKCNGKFPCASCEIYSCECTFSTRQGGARIKNLHKTSLEGTTVQVKEETDSSSTSFSNPQRCTDGPCAVEQPTKFFENFKLGGRSSGDNSGSDGKNDDDVNRNGFYEDDSESQATLTSLQTTLKNLKEMAHLGTHVTSAIESIELQISDLLKRWEPKVRTKELATTKFYPNKSIETQLMKNKYCDVVHLTRYAAWSNNKKDQDTSSQPLIDEIFGLYSPFQFLSLQGIGKCFQNYRSKSKCEIFPRTAKETIYIMLRFFDVCFHHINQGCVSIANPLENYLQKMNLLPSTPSSISSAGSPNTAHTKSHVALVINHLPQPFVRNITGISNSELLSEMNNDISMFGILLKMLDMHKNSYQNFLMEITSNPSVAKNTQSIDVLQEFIHYCQAGEALIALCYSYYNSTLYNYVDFTCDITHLEQLLYFLDLLFWLSEIYGFEKVLNVAVHFVSRVGLSRWEFYVGLDENFAERRRNLWWKAFYFEKTLASKLGYPSNIDDSKINCLLPKNFRDVGFLDNRDFIENVHLVRRSEAFDNMCISDLKYYGELAVLQIVSHFSSSVLFNEKFTSIRNTSKPSVVREKLLFEVLEIFNETEMKYDAIKEQTGKLFDIAFSKDSTELKVSREDKIMASKFVLFYEHHFCRMVNESDNIVARLCVHRRPSILIENLKIYLHKIYKSWTDMNKILLDFDNDYSVYRSFAHYSISCIILVSQAFSVAEFIKVNDVVNMIRVFKRFLDIKIFSENETNEHVFNSQSFKDYTRAFSFLTIVTRIMLLAYGESSSTNLDVISKYIDENAPDLKGIIELVLDTNSCAYRFLLEPVQKSGFHLTVSQMLKNRKFQEPLMSNEDNKQMKHNSGKNLNPDLPSLKTGTSCLLNGIESPQLPFNGRSAPSPVRNNSLPEFAQLPSFRSLSVSDMINPDYAQPTNGQNNTQVQSNKPINAQQQIPTSVQVPFMNTNEINNNNNNNNNNKNNINNINNNNSNNFSATSFNLGTLDEFVNNGDLEDLYSILWSDVYPDS 391BA6C52770436C tcb3_yeast Tricalbin-3 YML072C TCB3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTGIKAQVHPPPDSTLFHEEEKKKVGGNLPQKVINQQERGSDHAPSGHHQYHQLINHDANDTKTSNSVSDVSKGQKTADSNPEGKKQSSKDIFVASSAQKTNQLPGPNPQGSIGAVPLEGLRPKEFRSAPSRKPNKFDTSITKPGVLDDLGKLDEKDIKEKFHLDSDDKLFPWQNVGEFHASGKGSPNTKMSRVIKAYILENFYNDWYCNIATVLGTCFFSWLFAYIGFSWWSMIFIFLGTATVYNAEYTRFNRNIRDDLKRVTVEETLSDRVESTTWLNSFLSKFWVIYMPVLSQQVKDNVNPQLAGVAPGYGIDALAIDEFTLGSKAPTIKGIKSYTKTGKNTVEMDWSFAFTPSDVSDMTATEAREKINPKISLGVTLGKSFVSKTMPILVEDINVAGKMRIKVEFGKAFPNIKIVSLQLLEPPLIDFALKPIGGDTLGLDVMSFLPGLKSFVKNIINSNIGPMLFPPNHLDINVEDIMAAQSKEAIGVLAVTIASADSLKGSDFITNTVDPYIVMTTEDAVPGTDEEVRTSIKSNVKNPRWNETKYLLLNTLEQKLNLKCFDFNDVRKDTVIGDLQLDLADLLQNPVLDNQTAELRSGTKSKGILHYSLHWFPVKEDKSEEKAVERAEAKAKGKKEDENEDTTEKEEDENEESSQTDVGIAKITLQKVKYLDTTSSMTGSLSPCAELFIDGQKVKSYRTLRRINEPSWNETIEVLVPSKSNSKFVLKIFDDRMNGKALICEYSSSLDDIMTTLDTAQEFVKGSPQGDIYLDVSWKSIEMTGAFAAANSVSEPIGCIKLDVKDAIIKGDLSGVGDVDPYYTVSLNRRVLYKSIYHSDTDHPIFDNSTYVPIFSPNQILTLEFHDYQKIGKDRFIGSVQIPTSNVFKKDPKSGKYVGNNGKEEISKLKLKDHEHKVTESIVNVSTTFIPINLVYSPEELVNVEKLEKELKEKKKKFEATQEENEQEMEKNPKEWEVAEIEDPFDSDEKKINRKAKLSLNELIKQKSGILSMQILEGTLSPSSAYLEILADDISYPVFICMKPSQGKLNSEMANIFIRDLNYSKLHFRVSKKHIAKDSDDVISETSYSTLKLLKQAYEEPMWLNFNGSKMKVRFLYTPTSVKLPSSESVEDTGYLNIKLISGHGLKSADRNGYSDPFVHIFVNDKKVFKSNIKKKTLDPVWNEDAKIPILSRSKNQVIFNVLDWDRAGDNDDLGQASLDVSSLEVGKTYNWNLNLNTQGSIKLQGSFNPEYIKPSFDIVKGGITDKPMKIASGAAHATVGIAGTGIGAATGVATGGLKKGGHLLKSLGGNPMKRSKSSNGNESNGAKKSSEKKSFDRRSPSNLNSTSVTPRASLDYDPSVPNTSYAPVQSASPVVKPTDNTSSSSNKKDTPSSNSRGHSRASSFARTLAPHGTYNGFITVVAAENVAKHVQIKISLTQGGRLKHIYKTKSQKANNDGVAVFDEECSFKASPEANLVLGAISHQRLSRDKDLGIAQINLGDPQIQQDGQISVKLGDGHLIVKINYGKDKNGQVPPVPEVPQEYTQ 77A0AE57F9259784 tcb2-tcb1 779774 n/a 779775 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 779776 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 779783 779781 physical association physical association false false false No figure. tcb2-pdr1 779774 n/a 779777 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 779776 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false No figure. tcb2-tcb3 779774 n/a 779778 unspecified role unspecified role prey prey c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n/a 779776 unspecified role unspecified role bait bait c-terminus binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false No figure.