UniProt UniProt http://www.uniprot.org lin-2002b-1 Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lin H., Nguyen P., Vancura A. vancuraa@stjohns.edu yeast genomic library Mol. Genet. Genomics 267 (313-320) 2002 lin-2002b-2 Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lin H., Nguyen P., Vancura A. vancuraa@stjohns.edu yeast genomic library Mol. Genet. Genomics 267 (313-320) 2002 lin-2002b-3 Phospholipase C interacts with Sgd1p and is required for expression of GPD1 and osmoresistance in Saccharomyces cerevisiae. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Lin H., Nguyen P., Vancura A. vancuraa@stjohns.edu yeast genomic library Mol. Genet. Genomics 267 (313-320) 2002 sgd1_yeast Suppressor of glycerol defect protein 1 SGD1 YLR336C protein protein yeast Saccharomyces cerevisiae Baker's yeast MQKTDGIRIPGVILDELKTLDYSQDERFSISEGKKRRRGNGKHLSRKEKRKMERADKKRKIISTREINSSRLKSAPTSEKRSANAGVKNVGKQANGKNPISSDESESNENWDSDEVLTDEVAEESGEQAMSAEETMKKLESLKRKAKGIQGAENSGEIKGNSYEKKHIRNRDTNENFVSYPLAPSDRSAFERDEMDMQYYAKKLGLKGERKAIHAKDEFDAIGGLLEGLEYFENYGKSDEEYGDFATETNSMRKDDEASEKAFSSDDDLSASDFEDSDGLSESDNDSVADSDDNYRREKENPYVAPTQSVESYVPPSLRKKLDDSENNSTLSEISKKVNSSLNKLSDSNITIIITDLNRLYDSLPRQYVTESLTKGILNIISQNQKLLDGFIMNYAALAYTLSKLRGIEVGAFFIQKTVEAFLHHYEEEMENILKDQQSKISSKICINIATLLSYCYNFGFVSCRLIYDIIRIFVADPNEFTTELLLRIISISGQLIRGDDPSALRDIRSELLKNAKNLKEQSPRLRFLMDTMSDLKNNRLKPSILATDHHPLKKNLQSILNSSSSWEPLQVSLEDIKNIDSKGKWWLVGASWRGNMENAFEVSINNENDASKSKKSKISIEDDLLDDIPDWNIIARQQRMNTDIRRAIFISIMSAQDYLDAFSKLEKLSLKNKQVLEIPRIVLHCLLADSGSNGYNHYYALVANKICERYSHLSKSFQFLFWDVIKKFEDKEFDSESDTDEEDDLDDKEKLLRISNQGRFFGSLLANDILKLDVFKHVPFMGGLNTEGMLFMEILLFQLFLTVAKKSEKKLKMDESGNKRIIYSDDYLRDVLTKNVKSENMLFILKGLKWFINKKFRYHNFLAGKKGDKAFDRDERRLAWASKAAKSIIDKELENIDS 8EEFDB8258407FF3 plc1_yeast 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase 1 PLC1 Phosphoinositide phospholipase C Phospholipase C-1 YPL268W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTESAIDDQRFNLTKELQRHSCRDQGKITQKDDALDFISYSSFQSSFNTDQKSANNGSTVRRSIRSIFRRAAELPRVHMGPLTYSHGINELVNKKLRKDCDLSTLCRVLQRGIRMIRMTRRRRKFYEFKLINNNGQIIWKDGSKYLELDSVKDIRIGDTASTYQEEVDPKRLRSDSKLWIAIIYKVSNKLKALHVVALNELDFNTFLSCICGLVKLRRELMESILLPDNSQFARIHWQITVSEKEEDEKKDTLSFADVKKLCDKFHIYVSTGQLLEFFQLADINHNGLLNYFEFEKFIKILKNRKEVNMIWSKFTKPPHSHLSFENFFQFLITEQHEQVDRQTAWSYFIKYREPTQLTMGQDGFTKFLKEQPYLVEVKEELYSKPLNHYFIASSHNTYLLGKQIAETPSVEGYIQVLQQGCRCVEIDIWDGENGPVVCHGFLTSAIPLKTVIRVIKKYAFITSPYPLIISLEINCNKDNQKLASLIMREVLAEQLYFVGTRTDKLPSPRELKHKILLKSKKTSEATRGLSVNEPFPSSFSSSYESANEQELRMKDDSTNSSSATNSSSMQRIKRIGLKKHADIINDVSNISGIHGIKFRNFSLPESKTIAHCFSLNERKVEYMIKDKHLKLSLDKHNRRYLMRVYPHVLRYKSSNFNPIPFWKAGVQMVATNWQTNDIGQQLNLAMFQILDHQPDGSFKSGYVLKPKKLLPVVTKAKMIPLIYEHFENGSDPVTVKIRILSTQLLPRLNDTSPSRNNTNSFVKVEFHTDDEPTMPISIDKGTRISATEASTKSSQGNGFNPIWDAEVSITLKDTDLTFIKFMVISEETQIASVCLKLNYLRMGYRHIPLFNMEGEQYIFCTLFIHTQIL plc1-sgd1 757081 N/A 757084 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 757085 unspecified role unspecified role bait bait physical association physical association false false false No figure plc1-sgd1-1 757082 N/A 757085 unspecified role unspecified role bait bait N/A 757084 unspecified role unspecified role prey prey physical association physical association false false false Fig. 1A plc1-sgd1-2 757083 N/A 757085 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 757084 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 1B