UniProt UniProt http://www.uniprot.org byrd-2001-1 UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Byrd DT., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y. jin@biology.ucsc.edu Neuron (0896-6273) 2001 Only protein-protein interactions imex curation byrd-2001-2 UNC-16, a JNK-signaling scaffold protein, regulates vesicle transport in C. elegans. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Byrd DT., Kawasaki M., Walcoff M., Hisamoto N., Matsumoto K., Jin Y. jin@biology.ucsc.edu Neuron (0896-6273) 2001 Only protein-protein interactions imex curation jnk1_caeel Stress-activated protein kinase jnk-1 jnk-1 B0478.1 protein protein caeel Caenorhabditis elegans MEERLSTTSSYPSHPGRSVEEDHNTLLASSSISSIIRGTRGHLNNFIESVGNWLVPSSSGRDDDAVSLDSCQSVYSPVRHHINSGTGGGILMEPSSIHVPENYYSVTIGEAQMVVLKRYQNLRLIGSGAQGIVCSAFDTVRNEQVAIKKLSRPFQNVTHAKRAYRELKLMSLVNHKNIIGILNCFTPQKKLDEFNDLYIVMELMDANLCQVIQMDLDHERLSYLLYQMLCGIRHLHSAGIIHRDLKPSNIVVRSDCTLKILDFGLARTAIEAFMMTPYVVTRYYRAPEVILGMGYKENVDVWSIGCIFGELIRGRVLFPGGDHIDQWTRIIEQLGTPDRSFLERLQPTVRNYVENRPRYQATPFEVLFSDNMFPMTADSSRLTGAQARDLLSRMLVIDPERRISVDDALRHPYVNVWFDEIEVYAPPPLPYDHNMDVEQNVDSWREHIFRELTDYARTHDIYS jip_caeel JNK-interacting protein unc-16 JNK MAP kinase scaffold protein Uncoordinated protein 16 egl-39 ZK1098.10 protein protein caeel Caenorhabditis elegans MACNLSPVNEMADSITSSTPSEIVYGGPGSPDEHRTMSDKVQTMASAIYRELETMIKVHGEDGVKTLMPLVVNVLEALDLAYLERDEQTAELEMLKEDNEQLQTQYEREKALRKQTEQKYIEIEDTLIGQNKELDKKIESLESIMRMLELKAKNATDHASRLEEREVEQKLEFDRLHERYNTLLRTHVDHMERTKYLMGSEKFELMQNMPLPNMQLRNKMGMAASVDASSIRGVSDLISAHMTQSTTMDVNLANHITNEDWQDEFSSDIEPSPRDIPQSSADALTSPITTKEPTPKREAASPKQSEEEEADETTSVDPKENNDLLGADLTDDESDWNGLGLIPRRHRPNEMLDDDDTSDDGSLGMGREVENLIKENSELLDMKNALNIVKNDLINQVDELNSENMILRDENLSRQMVSEKMQEQITKHEEEIKTLKQKLMEKENEQEEDDVPMAMRKRFTRSEMQRVLMDRNAYKEKLMELEESIKWTEMQRAKKMQQQQQNVNQKKSGGIWEFFSSLLGDSVTPPASSRGNRASSSRGKMTRSVEYIDPDMISERRAAERREQYKLVREHVKKEDGRIEAYGWSLPNVEAEVSSVPIPVCCRPLLDNEPSLKIWCATGVVLRGGRDERGQWIVGDPIYFAPASMKKTKTSNHRPELEDEIKRARNLDARESELDEWQSSSLVWVVSSNQGKSLIAVLDANNPNNIIETFPACDSHLLCIQAVSGVMEGEPEMNEEQSKKYLSGGGKIKDLPEGLDGTDLGACEWVELRKMEDSEDGVPTYCSNDMKPSPKRTRDFSISEVAPVDSSAPVKEDPLPPPANRPGGRAALPPHIRDAMSKYDGVSGQMSGALPTVWMGGQNQYIYIHSAVTAWKQCLRRIKMPDAVLSIVHYKSRIFAALANGTIAIFHRNKHGEWSDEGYHSLRVGSATSSVRSLCLVSTNIWATYKNCVVVLDAESLQIVKVFAAHPRKDSQVRNMQWVGAGVWLSIRLDSTLRLYHAHTYEHLQDVDIEPYVTKMLGTSKLDFSYMRTTALLVSNRRLWIGTGTGVIISVPFSGQLEKKIETKDSKRPAGPGGLVRVYGATSENATNDEKTNDDFIPYCNLAHAQLSFHGHKDSVKFFLGVPGASKNGEDESAEVTLRRMLIMSGGDGYIDFRIGEENEPELTGQSIRPRDMSHLIIWEVDAELPILSK jkk1_caeel Dual specificity mitogen-activated protein kinase kinase jkk-1 jkk-1 JNK-1 activator kinase F35C8.3 protein protein caeel Caenorhabditis elegans MENVCFQQRLRDLETRVRKWKFLKLGLTEVRLRPRDRRSTSVDQKHKECSSTSSSPQHQRPNNIGYLTSPMERKFTPLSMKPSPSRRDTEKDALEYEFLEGYKKSGTLEIDGEKQVVDPNEIHIISLLGSGSCGVVESATVRSKLMAVKTMYKNDNKENLKRILRDVRIMSMCNSPFIVTSYGYFMFDSSVKICMQIMSACCEKLLRRIYHSKLDFFPEFVAGHIVYSAISALDYLKEKHSIIHRDIKPSNILFDDSGNVKLCDFGISGFMTDSMAHSKSAGCPPYMAPERLTIETNSKYDVRSDVWSLGITVYQLVTGLYPFPLNDMEFTTLTIIANLNLPSPSLREETKRSFSPLFIEFLDLCLRKDVRERPEYRQLMKHDFYLDYDPASGSAYKFKAINGKCNQVADWFVDVIRLSKTEDELKSIPNTPCVN q95y19_caeel Y41D4B.18 protein protein caeel Caenorhabditis elegans MARDPNFFVYSIQDAEKVISRSTNSETTLVLLNSKLDSSRIENLIQPDSTKLQFYRGIPDYDYNDTQSERAAQSLIFANPIKSWSSGNTYFFNTIEPMQYSLRALYIRVISFQISHEWKDPSYYKTTAPTTTGFLMSREVSTPSSNVDFMFNSGHNGIVGLLVSTALFADANATFSNNLEPDTLRLHPDPAIISRVFEWYSNATNLYVTPSQGVYYIQYFRADLNEPTTTSTAYAAPSSTASTSTPAPTNLPTTTGAPTNSTTNPPTATSPKTSSQPVSSSTSSTSSPAPTNPPTTSRPTTASQSTASQNVETTTKLGLTASITVSIMTTLLHKILFA The sequence has been withdrawn from uniprot. jun_human Transcription factor AP-1 JUN Activator protein 1 Proto-oncogene c-Jun V-jun avian sarcoma virus 17 oncogene homolog p39 protein protein human Homo sapiens Human MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLKLASPELERLIIQSSNGHITTTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGALSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKRMRNRIAASKCRKRKLERIARLEEKVKTLKAQNSELASTANMLREQVAQLKQKVMNHVNSGCQLMLTQQLQTF jnk1-unc16-1 765558 N/A 765560 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 765561 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 4A jkk1-unc16-1 765558 N/A 765561 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 765562 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 4A sek1-unc16-1 765558 N/A 765563 unspecified role unspecified role bait bait n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A-x-x 765561 unspecified role unspecified role prey prey n-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig 4A jnk1-jun-1 765559 N/A 765564 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 765560 enzyme enzyme unspecified role unspecified role c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false caeel Caenorhabditis elegans phosphorylation phosphorylation reaction phosphorylation false false false Phosphorylation of c-JUN. Fig 4B jnk1-jun-2 765559 N/A-x 765560 enzyme enzyme unspecified role unspecified role n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A-x 765564 enzyme target enzyme target substrate substrate unspecified role unspecified role n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false Phosphorylation of c-JUN. Fig 4C