IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ healy-2001-1 The Arabidopsis D-type cyclins CycD2 and CycD3 both interact in vivo with the PSTAIRE cyclin-dependent kinase Cdc2a but are differentially controlled. arath-cell-culture Arabidopsis thaliana cell culture plant_cell_culture plant cellular culture The culture conditions should always be cited in the experimental modifications annotation Either whole plant or undifferentiated plant cells maintained in culture. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Healy JM., Menges M., Doonan JH., Murray JA. Coimmunoprecipitations were carried out using extracts from exponentially growing and quiescent cells. j.murray@biotech.cam.ac.uk J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation ccd21_arath Cyclin-D2-1 CYCD2-1 CYCD2 F14M13.11 At2g22490 G1/S-specific cyclin-D2-1 Cyclin-delta-2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAENLACGETSESWIIDNDDDDINYGGGFTNEIDYNHQLFAKDDNFGGNGSIPMMGSSSSSLSEDRIKEMLVREIEFCPGTDYVKRLLSGDLDLSVRNQALDWILKVCAHYHFGHLCICLSMNYLDRFLTSYELPKDKDWAAQLLAVSCLSLASKMEETDVPHIVDLQVEDPKFVFEAKTIKRMELLVVTTLNWRLQALTPFSFIDYFVDKISGHVSENLIYRSSRFILNTTKAIEFLDFRPSEIAAAAAVSVSISGETECIDEEKALSSLIYVKQERVKRCLNLMRSLTGEENVRGTSLSQEQARVAVRAVPASPVGVLEATCLSYRSEERTVESCTNSSQSSPDNNNNNNNSNKRRRKQ cdka1_arath Cyclin-dependent kinase A-1 CDKA-1 Cell division control protein 2 homolog A CDC2 At3g48750 CDC2A T21J18.20 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP ccd31_arath Cyclin-D3-1 CYCD3-1 CYCD3 F28A23.80 At4g34160 G1/S-specific cyclin-D3-1 Cyclin-delta-3 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAIRKEEESREEQSNSFLLDALYCEEEKWDDEGEEVEENSSLSSSSSPFVVLQQDLFWEDEDLVTLFSKEEEQGLSCLDDVYLSTDRKEAVGWILRVNAHYGFSTLAAVLAITYLDKFICSYSLQRDKPWMLQLVSVACLSLAAKVEETQVPLLLDFQVEETKYVFEAKTIQRMELLILSTLEWKMHLITPISFVDHIIRRLGLKNNAHWDFLNKCHRLLLSVISDSRFVGYLPSVVAAATMMRIIEQVDPFDPLSYQTNLLGVLNLTKEKVKTCYDLILQLPVDRIGLQIQIQSSKKRKSHDSSSSLNSPSCVIDANPFNSDESSNDSWSASSCNPPTSSSSPQQQPPLKKMRGAEENEKKKPILHLPWAIVATP cycd2-cdc2a 772531 n/a 772532 unspecified role unspecified role bait bait n/a 772533 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 Figure 6B The authors noted from these experiments that "it is therefore impossible to determine whether CycD2 and Cdc2a are associated in quiescent cells, since CycD2 is not immunoprecipitated". cycd3-cdc2a 772531 n/a 772534 unspecified role unspecified role bait bait n/a 772533 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 cdc2a-cycd2 772531 n/a 772533 unspecified role unspecified role bait bait n/a 772532 unspecified role unspecified role prey prey physical association physical association false false false Figure 6C The authors noted from these experiments "that the majority of CycD2 present in quiescent cells is not associated with Cdc2a".