IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ lei-1999-1 Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lei S., Pulakat L., Gavini N. ngavini@bgnet.bgsu.edu J. Bacteriol. (0021-9193) 1999 Only protein-protein interactions imex curation nifl_azovi Nitrogen fixation regulatory protein nifL protein protein azovi Azotobacter vinelandii MTPANPTLSNEPQAPHAESDELLPEIFRQTVEHAPIAISITDLKANILYANRAFRTITGYGSEEVLGKNESILSNGTTPRLVYQALWGRLAQKKPWSGVLVNRRKDKTLYLAELTVAPVLNEAGETIYYLGMHRDTSELHELEQRVNNQRLMIEAVVNAAPAAMVVLDRQHRVMLSNPSFCRLARDLVEDGSSESLVALLRENLAAPFETLENQGSAFSGKEISFDLGGRSPRWLSCHGRAIHIENEQAHVFFAPTEERYLLLTINDISELRQKQQDSRLNALKALMAEEELLEGMRETFNAAIHRLQGPVNLISAAMRMLERRLGDKAGNDPVLSAMREASTAGMEALENLSGSIPVRMAESKMPVNLNQLIREVITLCTDQLLAQGIVVDWQPALRLPWVMGGESSQRSMIKHLVDNAIESMSQNQVSRRELFISTRVENHLVRMEITDSGPGIPPDLVLKVFEPFFSTKPPHRVGRGMGLPVVQEIVAKHAGMVHVDTDYREGCRIVVELPFSAST nifa_azovi Nif-specific regulatory protein nifA protein protein azovi Azotobacter vinelandii MNATIPQRSAKQNPVELYDLQLQALASIARTLSREQQIDELLEQVLAVLHNDLGLLHGLVTISDPEHGALQIGAIHTDSEAVAQACEGVRYRSGEGVIGNVLKHGNSVVLGRISADPRFLDRLALYDLEMPFIAVPIKNPEGNTIGVLAAQPDCRADEHMPARTRFLEIVANLLAQTVRLVVNIEDGREAADERDELRREVRGKYGFENMVVGHTPTMRRVFDQIRRVAKWNSTVLVLGESGTGKELIASAIHYKSPRAHRPFVRLNCAALPETLLESELFGHEKGAFTGAVKQRKGRFEQADGGTLFLDEIGEISPMFQAKLLRVLQEGEFERVGGNQTVRVNVRIVAATNRDLESEVEKGKFREDLYYRLNVMAIRIPPLRERTADIPELAEFLLGKIGRQQGRPLTVTDSAIRLLMSHRWPGNVRELENCLERSAIMSEDGTITRDVVSLTGVDNESPPLAAPLPEVNLADETLDDRERVIAALEQAGWVQAKAARLLGMTPRQIAYRIQTLNIHMRKI nifa-nifl 770105 n/a 770106 unspecified role unspecified role prey prey n/a 770107 unspecified role unspecified role bait bait physical association physical association false false false table1 and figure 3 nifa-nifl-1 770105 n/a 770107 unspecified role unspecified role prey prey n/a 770106 unspecified role unspecified role bait bait physical association physical association false false false table1 and figure 3