IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ zariwala-1998-1 Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Zariwala M., Liu J., Xiong Y. yxiong@unc.edu human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation zariwala-1998-2 Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Zariwala M., Liu J., Xiong Y. yxiong@unc.edu human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation zariwala-1998-5 Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. human-cls Homo sapiens fibroblast cell line (GM03318) cls Human fibroblast obtained from patients with Coffin-Lowry Syndrome (CLS). GM03318 (NIGMS Human Genetic Cell Repository) Catalog ID GM03318 (NIGMS Human Genetic Cell Repository) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation zariwala-1998-3 Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. human-ct10 Homo sapiens ct10 SV40 transformed human fbroblasts strains CT10 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation zariwala-1998-4 Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zariwala M., Liu J., Xiong Y. yxiong@unc.edu human HaCaT keratinocyte cell cDNA Oncogene (0950-9232) 1998 Only protein-protein interactions imex curation cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL ccne2_human G1/S-specific cyclin-E2 CCNE2 protein protein human Homo sapiens Human MSRRSSRLQAKQQPQPSQTESPQEAQIIQAKKRKTTQDVKKRREEVTKKHQYEIRNCWPPVLSGGISPCIIIETPHKEIGTSDFSRFTNYRFKNLFINPSPLPDLSWGCSKEVWLNMLKKESRYVHDKHFEVLHSDLEPQMRSILLDWLLEVCEVYTLHRETFYLAQDFFDRFMLTQKDINKNMLQLIGITSLFIASKLEEIYAPKLQEFAYVTDGACSEEDILRMELIILKALKWELCPVTIISWLNLFLQVDALKDAPKVLLPQYSQETFIQIAQLLDLCILAIDSLEFQYRILTAAALCHFTSIEVVKKASGLEWDSISECVDWMVPFVNVVKSTSPVKLKTFKKIPMEDRHNIQTHTNYLAMLEEVNYINTFRKGGQLSPVCNGGIMTPPKSTEKPPGKH cks1_human Cyclin-dependent kinases regulatory subunit 1 PNAS-16 PNAS-143 CKS1B CKS1 protein protein human Homo sapiens Human MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK cdkn3_human Cyclin-dependent kinase inhibitor 3 CDKN3 CDI1 CIP2 KAP CDK2-associated dual-specificity phosphatase Kinase-associated phosphatase Cyclin-dependent kinase-interacting protein 2 Cyclin-dependent kinase interactor 1 protein protein human Homo sapiens Human MKPPSSIQTSEFDSSDEEPIEDEQTPIHISWLSLSRVNCSQFLGLCALPGCKFKDVRRNVQKDTEELKSCGIQDIFVFCTRGELSKYRVPNLLDLYQQCGIITHHHPIADGGTPDIASCCEIMEELTTCLKNYRKTLIHCYGGLGRSCLVAACLLLYLSDTISPEQAIDSLRDLRGSGAIQTIKQYNYLHEFRDKLAAHLSSRDSQSRSVSR ccnd1_human G1/S-specific cyclin-D1 CCND1 BCL1 PRAD1 PRAD1 oncogene BCL-1 oncogene B-cell lymphoma 1 protein protein protein human Homo sapiens Human MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI ccnd2_human G1/S-specific cyclin-D2 CCND2 protein protein human Homo sapiens Human MELLCHEVDPVRRAVRDRNLLRDDRVLQNLLTIEERYLPQCSYFKCVQKDIQPYMRRMVATWMLEVCEEQKCEEEVFPLAMNYLDRFLAGVPTPKSHLQLLGAVCMFLASKLKETSPLTAEKLCIYTDNSIKPQELLEWELVVLGKLKWNLAAVTPHDFIEHILRKLPQQREKLSLIRKHAQTFIALCATDFKFAMYPPSMIATGSVGAAICGLQQDEEVSSLTCDALTELLAKITNTDVDCLKACQEQIEAVLLNSLQQYRQDQRDGSKSEDELDQASTPTDVRDIDL ccnh_human Cyclin-H MO15-associated protein p37 p34 CCNH protein protein human Homo sapiens Human MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAFLACKVDEFNVSSPQFVGNLRESPLGQEKALEQILEYELLLIQQLNFHLIVHNPYRPFEGFLIDLKTRYPILENPEILRKTADDFLNRIALTDAYLLYTPSQIALTAILSSASRAGITMESYLSESLMLKENRTCLSQLLDIMKSMRNLVKKYEPPRSEEVAVLKQKLERCHSAELALNVITKKRKGYEDDDYVSKKSKHEEEEWTDDDLVESL cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP mpip2_human M-phase inducer phosphatase 2 Dual specificity phosphatase Cdc25B CDC25B CDC25HU2 protein protein human Homo sapiens Human MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ cdk3_human Cyclin-dependent kinase 3 CDK3 Cell division protein kinase 3 CDKN3 protein protein human Homo sapiens Human MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT cdn1c_human Cyclin-dependent kinase inhibitor 1C Cyclin-dependent kinase inhibitor p57 p57Kip2 CDKN1C KIP2 protein protein human Homo sapiens Human MSDASLRSTSTMERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTEVDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVPAPASTPPPVPVLAPAPAPAPAPVAAPVAAPVAVAVLAPAPAPAPAPAPAPAPVAAPAPAPAPAPAPAPAPAPAPDAAPQESAEQGANQGQRGQEPLADQLHSGISGRPAAGTAAASANGAAIKKLSGPLISDFFAKRKRSAPEKSSGDVPAPCPSPSAAPGVGSVEQTPRKRLR ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA cdk2-ccne2-1 Interacton between CDK2 and Cyclin E2 identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605715 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-cks1-1 Interacton between CDK2 and CKS1 identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605716 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-cdkn3-2 Interacton between CDK2 and CDKN3 identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605717 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-ccnd1-1 Interacton between CDK2 and CCND1 identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605718 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-ccnd2-1 Interacton between CDK2 and CCND2 identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605719 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-ccnh-1 Interacton between CDK2 and CCNH identified by Y2H 605709 N/A 605720 unspecified role unspecified role prey prey N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false CDK2 is catalytically inactive. cdk2-cdkn1a-1 Interacton between CDK2 and CDKN1A identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605721 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-cdc25b Interacton between CDK2 and CDC25B identified by Y2H 605709 N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605722 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. cdk2-ccne2-2 Interacton between CDK2 and Cyclin E2 identified by Y2H 605710 N/A 605715 unspecified role unspecified role prey prey N/A 605714 unspecified role unspecified role bait bait P24941:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false CDK2 is catalytically inactive. 1a cdk3-ccne2-1 Interacton between CDK3 and Cyclin E2 identified by Y2H 605710 N/A 605723 unspecified role unspecified role bait bait Q00526:p.Lys33Met mutation mutation certain certain sequence position certain certain certain sequence position certain false N/A 605715 unspecified role unspecified role prey prey physical association physical association false false false CDK2 is catalytically inactive. 1a cdkn1a-ccne2-1 Interacton between CDKN1A and Cyclin E2 identified by Y2H 605710 N/A 605715 unspecified role unspecified role prey prey N/A 605721 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A cdkn1b-ccne2-1 Interacton between CDKN1B and Cyclin E2 identified by Y2H 605710 N/A 605715 unspecified role unspecified role prey prey N/A 605724 unspecified role unspecified role bait bait cyclin bind region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3A cdkn1c-ccne2-1 Interacton between CDKN1B and Cyclin E2 identified by Y2H 605710 N/A 605725 unspecified role unspecified role bait bait N/A 605715 unspecified role unspecified role prey prey physical association physical association false false false 3A cdk2-ccne2-4 Interacton between CDK2 and Cyclin E2 identified by coimmunoprecipitation 605711 N/A 605714 unspecified role unspecified role prey prey N/A 605715 unspecified role unspecified role bait bait physical association physical association false false false 7a A competing cyclin E2 antigen peptide The half-life of the short lived Cyclin E2 protein is increased by expression of the viral E6 and E7 oncoproteins. cdk2-ccne1-4 Interacton between CDK2 and Cyclin E1 identified by coimmunoprecipitation 605711 N/A 605726 unspecified role unspecified role bait bait N/A 605714 unspecified role unspecified role prey prey physical association physical association false false false 7b A competing cyclin E2 antigen peptide The half-life of the short lived Cyclin E1 protein is increased by expression of the viral E7 oncoproteins. cdk2-ccne2-3 Interacton between CDK2 and Cyclin E2 identified by coimmunoprecipitation 605712 N/A 605714 unspecified role unspecified role prey prey N/A 605715 unspecified role unspecified role bait bait physical association physical association false false false 2b A competing cyclin E2 antigen peptide cdkn1b-ccne2-2 Interacton between CDKN1B and Cyclin E2 identified by coimmunoprecipitation 605713 N/A 605724 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 605715 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 3b