UniProt UniProt http://www.uniprot.org tang-1997b-1 EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tang HY., Munn A., Cai M. mcbcaimj@leonis.nus.sg Mol. Cell. Biol. 17 (4294-4304) 1997 Only protein-protein interactions imex curation tang-1997b-2 EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Tang HY., Munn A., Cai M. mcbcaimj@leonis.nus.sg Mol. Cell. Biol. 17 (4294-4304) 1997 Only protein-protein interactions imex curation end3_yeast Actin cytoskeleton-regulatory complex protein END3 END3 YNL084C N2307 Endocytosis protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPKLEQFEIKKYWQIFSGLKPIENKVNHDQVLPILYNSKLDSSVLNKIWFLADIDDDDNLDFEEFVICMRLIFDMVNKNISSVPDELPDWLIPGSKVNLIKERKKRKQIENADLPPKKEIKVDWYMSPDDLNQYEKIYNSCAKLTDGTITFNELSTKLSTKFFNISKTDLNKVWSLINPQNLPSIDRDPTFYFIHCLRQRNDLGAEIPASLPNSLAEVCNKKQLSYDLRSSQPPTKRKEEANEVDNLRDNGQNSSSDSSGSNVLSNEDSIKQKYASLTDDQVANMREQLEGLLNYKKSEKTQGGSKLSKRINIRSITDDLDNIEQQVEVLENYLNNKRHELQALQAEIN pan1_yeast Actin cytoskeleton-regulatory complex protein PAN1 PAN1 MDP3 MIP3 YIR006C YIB6C DIM2 Mitochondrial distribution of proteins protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYNPYQQQGMGYQQQQQQQQQQPNGFYPQQQQGQSSNQPQGQPQPQQQMAFNQPQATGIGGMPQSFGNSFSSMPQQPQTGYNNNGNNGSVYGNGNFGQQPQQQQQQAKPQHTGYVPNSSMPMMNTTGTMPPPNPAQQPQLQSIQPQGTGYYQAANTANVHSVQPLQSQGTGYYVSTPNLISSNQTQQPLQAQGTGYYQSQPQQVPPPQQAQSLQPLKPQQTGFYLQPQNQAPLEPLKPTATGFVNSFANNGLNNDIKIPAIRLSFITAQDQAKFETLFRSIVTNGSNTVSGANCRKILMRSGLPPSQLARIWTLCDTSKAGELLFPEFALAMHLINDVLQGDTIPYELDSKTKNEVSSFIDAINLSIANQDSSANDAPKTPFDEFITAGVQNLQPQPTGYMPQTSFGIPLQSQITGGGVASALNPQSTGFMAPTTFNMSMNTGTPGLNPQITGGAPASMQPNITGNALQPQTTGMMPQTTGMMPQTTGMMPQTSFGVNLGPQLTGGALQSQYTGGYGSVMPQQSGPASMPNLSFNQQGLQSQLTGLQPQPTGFLPPSNFSATMPLTAQKTGFGNNEIYTKSNFNNNLIDNSSQDKISTEEKSLFYKIFETFDTQNKGLLDSPTAVEIFRKSGLNRADLEQIWNLCDINNTGQLNKQEFALGMHLVYGKLNGKPIPNVLPSSLIPSSTKLLDNLKNQLKTEPTTTKEKPSFGKIDALSYKNNDDDVLPNYRNRRKVYSAKNEEQSSFSSPSAKSVNHSSSTLQTDDISVDKTVEKKTAKPKYAGFSREINLKNIASLENEIKNISNPENCYDSSIPSDLTSRFDAIIAKLPNLFNEISTIDNEITNAKIQLYRKKNPSSIIGSGPNGEITENDRKKAKSRALLRARMSALTGKSTESEDSLSMEDEQQSAEIKRIQQENGKNQEIIKDIRSSISDISASLKSTMTGSNMISNQEFERWEFGIGLEDGVREFLDDLKSNSNKSVTESSPFVPSSTPTPVDDRSSSPSYSQFKTAEERAAYLKEQAKKRMKEKLAKFDKNRRNVTQSSRSISSENSREQPQQIAGSSNLVEPRATPFQEEKYVEVAQPTQPVQSTQPVQPTQPVQPTQPVQPTQPVQPTQPVQPTQPVQNVYNAKQESDDEDEDDEEKRLQEELKRLKLKKKADKEKRLAALRKQIEDAQNESDEEETNGKDNFGGHVNVPQAAPVAPSAAFSQNSTNAPRSVHAAVTPAAGKNSTGLPSTTMGHNPYFKDASASSTSTFDARAAEMQRRIQRGLDEDEDDGWSDEDESNNRVAVDNKVEEAKIGHPDHARAPPVTAAPLPSVTPVPPAVPVPQANTSNEKSSPIPIAPIPPSVTQEPPVPLAPPLPAVDGFQEPPIPSAPAIATAVQKSGSSTPALAGGVLPPPPPLPTQQASTSEPIIAHVDNYNGAEKGTGAYGSDSDDDVLSIPESVGTDEEEEGAQPVSTAGIPSIPPAGIPPPPPLP pan1-end3 680798 N/A 680800 unspecified role unspecified role bait bait N/A 680801 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4A pan1-end3-1 680798 N/A 680801 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false N/A 680800 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 4B pan1-end3-2 680799 N/A 680801 unspecified role unspecified role bait bait n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 680800 unspecified role unspecified role prey prey physical association physical association false false false Fig. 3A pan1-end3-3 680799 N/A 680801 unspecified role unspecified role prey prey n-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 680800 unspecified role unspecified role bait bait n-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 3B