IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kuznetsov-1994-2 Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. Immunoprecipitation was performed both on lysates from uncross-linked and cross-linked cells. A unique UniProtKB entry could not be associated with PDI since the exact gene product of the interactor cannot be ascertained. J. Biol. Chem. (0021-9258) 1994 kuznetsov-1994-1 Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kuznetsov G., Chen LB., Nigam SK. J. Biol. Chem. (0021-9258) 1994 kuznetsov-1994-3 Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. rat-pccl3 Rattus norvegicus thyroid follicular cell line PCCl3 pccl3 Rat thyroid follicular cell line PCCL3 cells are derived from rat thyroid follicular cells and are dependent on TSH for growth. They also retain differentiated properties as they trap iodide, express thyroglobulin, thyroid peroxidase, and the TSH receptor in a TSH-dependent manner. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. J. Biol. Chem. (0021-9258) 1994 kuznetsov-1994-4 Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. rat-frtl_5 Rattus norvegicus thyroid cell line FRTL-5 frtl_5 Rat thyroid cell line FRTL-5 This thyroid cell line has been established from normal thyroid glands from 5 to 6-week-old Fischer rats. FRTL-5 is a clonal line of the continuously cultured FRTL cells derived by successive clonal isolation. The cells are characterised by maintaining highly differentiated thyroid features such as secretion of thyroglobulin and concentration of iodide. They display a sensitivity to thyroid-stimulating hormone (TSH)-induced increase in cAMP levels detecting TSH as absolute growth requirement. density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Kuznetsov G., Chen LB., Nigam SK. J. Biol. Chem. (0021-9258) 1994 q0r337_rat Grp94 protein protein rat Rattus norvegicus (Rat) Rat MRVLWVLGLCCVLLTFG bip_rat Endoplasmic reticulum chaperone BiP Hspa5 Grp78 Immunoglobulin heavy chain-binding protein Steroidogenesis-activator polypeptide 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein rat Rattus norvegicus (Rat) Rat MKFTVVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSPEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSGGPPPTGEEDTSEKDEL pdia4_rat Protein disulfide-isomerase A4 Endoplasmic reticulum resident protein 72 Calcium-binding protein 2 Pdia4 Cabp2 Erp70 Endoplasmic reticulum resident protein 70 protein protein rat Rattus norvegicus (Rat) Rat MKLRKAWLLVLLLALTQLLAAASAEDAHEDASDSENPIEDDDDEEEDEEDEDDLEVKEENGVWVLNDENFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKKGQAVDYDGSRTQEEIVAKVREVSQPDWTPPPEVTLTLTKENFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVDYMVEQSGPPSKEILTLKQVQEFLKDGDDVVILGVFQGVGDPGYLQYQDAANTLREDYKFHHTFSTEIAKFLKVSLGKLVLMQPEKFQSKYEPRMHVMDVQGSTEASAIKDYVVKHALPLVGHRKTSNDAKRYSKRPLVVVYYSVDFSFDYRTATQFWRNKVLEVAKDFPEYTFAIADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDALQEFVMAFKKGKLKPVIKSQPVPKNNKGPVRVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPVYTSLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKFIDEHATKRSRTKEEL thyg_rat Thyroglobulin Tg protein protein rat Rattus norvegicus (Rat) Rat MMTLVLWVSTLLSSVCLVAANIFEYQVDAQPLRPCELQREKAFLKQDEYVPQCSEDGSFQTVQCQNDGQSCWCVDSDGTEVPGSRQLGRPTACLSFCQLHKQRILLSSYINSTDALYLPQCQDSGNYAPVQCDLQQVQCWCVDTEGMEVYGTRQQGRPTRCPRSCEIRSRRLLHGVGDKSPPQCDADGEFMPVQCKFVNTTDMMIFDLIHNYNRFPDAFVTFSAFRNRFPEVSGYCYCADSQGRELAETGLELLLDEIYDTIFAGLDQASTFTQSTMYRILQRRFLAIQLVISGRFRCPTKCEVEQFTATSFGHPYIPSCHRDGHYQTVQCQMERMCWCVDAQGIEIPGTRQQGQPLFCAKDQSCASERQQALSRLYFETPGYFSPQDLLSSEDRLVPVSGARLDISCPPRIKELFVDSGLLRSIAVERYQQLSESRSLLREAIRAIFPSRELAGLALQFTTNPKRLQQNLFGGTFLVNAAQLNLSGALGTRSTFNFSQFFQQFGLPGFLVRDRATDLAKLLPVSLDSSPTPVPLRVPEKRVAMNKSVVGTFGFKVNLQENQDALKFLVSLMELPEFLVFLQRAVSVPEDRARDLGDVMEMVFSAQACKQTSGRFFVPSCTAEGSYEDIQCYAGECWCVNSQGKEVEGSRVSGGHPRCPTKCEKQRAQMQNLAGAQPAGSSFFVPTCTSEGYFLPVQCFNSECYCVDAEGQVIPGTQSTIGEPKLCPSVCQLQAEQAFLGVVGVLLSNSSMVPPISSVYIPQCSTSGQWMPVQCDGPHEQVFEWYERWNTQNSDGQELTTATLLMKLMSYREVASTNFSLFLQSLYDAGQQSIFPVLAQYPSLQDVPQVVLEGATIQPGENIFLDPYIFWQILNGQLSQYPGPYSDFSMPLEHFNLRSCWCVDEAGQELDGTRTRAGEIPACPGPCEEVKFRVLKFIKETEEIVSASNASSFPLGESFLVAKGIQLTSEELGLPPLYPSREAFSEKFLRGSEYAIRLAAQSTLTFYQKLRASLGESNGTASLLWSGPYMPQCNTIGGWEPVQCHPGTGQCWCVDGWGELIPGSLMARSSQMPQCPTSCELSRANGLISAWKQAGHQRNPGPGDLFTPVCLQTGEYVRQQTSGTGAWCVDPSSGEGVPTNTNSSAQCPGLCDALKSRVLSRKVGLGYTPVCEALDGGFSPVQCDLAQGSCWCVLASGEEVPGTRVVGTQPACESPQCPLPFSGSDVTDGVVFCETASSSGVTTVQQCQLFCRQGLRNVFSPGPLICNLESQRWVTLPLPRACQRPQLWQTMQTQAHFQLLLPPGKMCSIDYSGLLQAFQVFILDELITRGFCQIQVKTFGTLVSRTVCDNSSIQVGCLTAERLGVNATWKLQLEDISVGSLPNLHSIERALMGQDLLGRFANLIQSGKFQLHLDSKTFSADTILYFLNGDRFVTSPMTQLGCLEGFYRVSTTSQDPLGCVKCPEGSFSQDGKCTPCPAGTYQGQAGSSACIPCPRGRTTTITGAFSKTHCVTDCQRDEAGLQCDQNGQYQANQKDMDSGEVFCVDSEGQRLQWLQTEAGLSESQCLMMRKFEKAPESKVIFDASSPVIVKSRVPSANSPLVQCLADCADDEACSFVTVSSMSSEVSCDLYSWTRDNFACVTSDQEEDAVDSLKETSFGSLRCQVKVRNSGKDSLAVYVKKGHEFTASGQKSFEPTGFQNVLSGLYSSVVFSALGTNLTDTHLFCLLACDQDSCCDGFIVTQVKEGPTICGLLSAPDILVCHINDWRDASDTQANGTCAGVTYDQGSRQMTMSLGGQEFLQGLTLLEGTQDSFISFQQVYLWKDSDIGSRPESMGCGRGMVPKSEAPEGADMATELFSPVDITQVIVNTSHSLPSQQYWLSTHLFSAEQANLWCLSRCAQEPVFCQLADIMESSSLYFTCSLYPEAQVCDNDVESNAKNCSQILPRQPTALFQRKVVLNDRVKNFYTRLPFQKLSGISIRDRIPMSEKLISNGFFECERLCDRDPCCTGFGFLNVSQMQGGEMTCLTLNSMGIQTCSEENGATWRILDCGSEDTEVHTYPFGWYQKPAVWSDAPSFCPSAALQSLTEEKVALDSWQTLALSSVIIDPSIKHFDVAHISISATRNFSLAQDFCLQECSRHQDCLVTTLQIQQGVVRCVFYPDIQSCEHSLRSKTCWLLLHEEAAYIYRKSGAPLHQSDGISTPSVHIDSFGQLQGGSQVVKVGTAWKQVYQFLGVPYAAPPLAENRFQAPEVLNWTGSWDATKLRSSCWQPGTRTPTPPQISEDCLYLNVFVPENLVSNASVLVFFHNTVEMEGSGGQLNIDGSILAAVGNLIVVTANYRLGVFGFLSSGSDEVAGNWGLLDQVAALTWVQTHIGAFGGDPQRVTLAADRGGADVASIHLLITRPTRLQLFRKALLMGGSALSPAAIISPDRAQQQAAALAKEVGCPNSSVQEVVSCFRQKPANILNEAQTKLLAVSGPFHYWGPVVDGQYLRELPSRRLKRPLPVKVDLLIGGSQDDGLINRAKAVKQFEESQGRTNSKTAFYQALQNSLGGEDSDARILAAAIWYYSLEHSTDDYASFSRALENATRDYFIICPIVNMASLWARRTRGNVFMYHVPESYGHGSLELLADVQYAFGLPFYSAYQGYFSTEEQSLSLKVMQYFSNFIRSGNPNYPHEFSQKAAEFATPWPDFVPGAGGESYKELSAQLPNRQGLKKADCSFWSKYIQTLKDADGAKDAQLTKSGEEDLEVGPGSEEDFSGSLEPVPKSYSK tg-hspa5-1 Tg coimmunoprecipitates BiP, grp94 and ERp72 in FRTL-5 lysates from cross-linked cells 676773 N/A 676777 unspecified role unspecified role prey prey N/A 676778 unspecified role unspecified role prey prey N/A 676779 unspecified role unspecified role prey prey N/A 676780 unspecified role unspecified role bait bait association association false false false 3 This interaction occurred in cells cross-linked using DSP. The amount of ERp72 coimmunoprecipitated with Tg was increased more than 10-fold in the cross-linked cells as compared to untreated cells. tg-hspa5-2 Tg coimmunoprecipitates BiP and ERp72 in FRTL-5 lysates from uncross-linked cells 676773 N/A 676779 unspecified role unspecified role prey prey N/A 676778 unspecified role unspecified role prey prey N/A 676780 unspecified role unspecified role bait bait association association false false false 3 These interactions occurred in uncross-linked cells. In the uncrossed-linked cell lysates grp94 was not coimmunoprecipitated with Tg. hspa5-tg-1 BiP coimmunoprecipitates Tg in FRTL-5 lysates 676773 N/A 676778 unspecified role unspecified role bait bait N/A 676780 unspecified role unspecified role prey prey physical association physical association false false false 1 C This interaction occurred inl ysates from cells cross-linked with DSP and untreated cells. tg-hspa5 Tg coimmunoprecipitates BiP in FRTL-5 lysates 676774 N/A 676778 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 676780 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 ATP and Mg2+ - Tg did not coimmunoprecipitate BiP when cells were lysed in the presence of 2 mM ATP and 2 mM MgCl2. This suggests that the dissociation of Tg and BiP requires an ATP-hydrolyzing reaction. hspa5-tg Tg coimmunoprecipitates with BiP in FRTL-5 lysates 676774 N/A 676778 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 676780 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2 ATP and Mg2+ - Tg did not coimmunoprecipitate with BiP when cells were lysed in the presence of 2 mM ATP and 2 mM MgCl2. This suggests that the dissociation of Tg and BiP requires an ATP-hydrolyzing reaction. pdia4-tg ERp72 coimmunoprecipitates Tg in PCCl3 lysates from cross-linked cells 676775 N/A 676780 unspecified role unspecified role prey prey N/A 676779 unspecified role unspecified role bait bait physical association physical association false false false 4 B This interaction only occurred in lysates from cells cross-linked with DSP. pdia4-grp94 ERp72 coimmunoprecipitates grp94 and BiP in PCCl3 lysates from cross-linked cells 676775 N/A 676778 unspecified role unspecified role prey prey N/A 676779 unspecified role unspecified role bait bait N/A 676777 unspecified role unspecified role prey prey association association false false false 4 D These interactions only occurred in lysates from cells cross-linked with DSP. grp94-hspa5 Tg, ERp72, grp94 and BiP cosediment in FRTL-5 lysates 676776 N/A 676779 unspecified role unspecified role neutral component neutral component N/A 676780 unspecified role unspecified role neutral component neutral component N/A 676778 unspecified role unspecified role neutral component neutral component physical association physical association false false false 5 The amount of ERp72 cosedimenting with Tg was increased in lysates from DSP cross-linked cells. The amount of grp74 cosedimenting with Tg was markedly increased in lysates from DSP cross-linked cells, and very little was cosedimented in untreated cells. The participant EBI-916036(P06761) was duplicated. It is posssible that originally this interaction did contain more participants pointing to different Trembl proteins which have been merged to a single swissprot protein later.