IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ michniewicz-2007-1 Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. jifri@psb.ugent.be Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation michniewicz-2007-3 Antagonistic Regulation of PIN Phosphorylation by PP2A and PINOID Directs Auxin Flux. in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Michniewicz M., Zago MK., Abas L., Weijers D., Schweighofer A., Meskiene I., Heisler MG., Ohno C., Zhang J., Huang F., Schwab R., Weigel D., Meyerowitz EM., Luschnig C., Offringa R., Friml J. Transgenic Arabidopsis plants expressing epitope tagged protein constructs were used in this study. The myelin basic protein was purchased from Sigma and the source organism is not stated. As this could be one of a number of source organisms avaliable this protein was not annotated in this entry. jifri@psb.ugent.be Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation 2aaa_arath Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform PP2AA1 EER1 RCN1 REGA F2J7.19 At1g25490 Protein enhancer of ethylene-response 1 Protein ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID 1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAMVDEPLYPIAVLIDELKNDDIQLRLNSIRRLSTIARALGEERTRKELIPFLSENSDDDDEVLLAMAEELGVFIPFVGGIEFAHVLLPPLESLCTVEETCVREKAVESLCKIGSQMKENDLVESFVPLVKRLAGGEWFAARVSACGIFHVAYQGCTDVLKTELRATYSQLCKDDMPMVRRAAASNLGKFATTVESTFLIAEIMTMFDDLTKDDQDSVRLLAVEGCAALGKLLEPQDCVARILPVIVNFSQDKSWRVRYMVANQLYELCEAVGPDCTRTDLVPAYVRLLRDNEAEVRIAAAGKVTKFCRLLNPELAIQHILPCVKELSSDSSQHVRSALASVIMGMAPILGKDSTIEHLLPIFLSLLKDEFPDVRLNIISKLDQVNQVIGIDLLSQSLLPAIVELAEDRHWRVRLAIIEYVPLLASQLGIGFFDDKLGALCMQWLQDKVYSIREAAANNLKRLAEEFGPEWAMQHLVPQVLDMVNNPHYLHRMMVLRAISLMAPVMGSEITCSKFLPVVVEASKDRVPNIKFNVAKLLQSLIPIVDQSVVDKTIRQCLVDLSEDPDVDVRYFANQALNSIDGSTAAQS pini_arath Auxin efflux carrier component 1 Protein PIN-FORMED PIN1 F6D5.2 At1g73590 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIAANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPLLKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSDIMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSATPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDGGPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGNAPVVGGKRQDGNGRDLHMFVWSSSASPVSDVFGGGGGNHHADYSTATNDHQKDVKISVPQGNSNDNQYVEREEFSFGNKDDDSKVLATDGGNNISNKTTQAKVMPPTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLLYYILLGL pin2_arath Auxin efflux carrier component 2 Auxin efflux carrier AGR Polar-auxin-transport efflux component AGR1 Protein AGRAVITROPIC 1 Ethylene-insensitive root 1 Protein WAVY 6 PIN2 AGR AGR1 EIR1 WAV6 MUL3.3 At5g57090 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MITGKDMYDVLAAMVPLYVAMILAYGSVRWWGIFTPDQCSGINRFVAVFAVPLLSFHFISSNDPYAMNYHFLAADSLQKVVILAALFLWQAFSRRGSLEWMITLFSLSTLPNTLVMGIPLLRAMYGDFSGNLMVQIVVLQSIIWYTLMLFLFEFRGAKLLISEQFPETAGSITSFRVDSDVISLNGREPLQTDAEIGDDGKLHVVVRRSSAASSMISSFNKSHGGGLNSSMITPRASNLTGVEIYSVQSSREPTPRASSFNQTDFYAMFNASKAPSPRHGYTNSYGGAGAGPGGDVYSLQSSKGVTPRTSNFDEEVMKTAKKAGRGGRSMSGELYNNNSVPSYPPPNPMFTGSTSGASGVKKKESGGGGSGGGVGVGGQNKEMNMFVWSSSASPVSEANAKNAMTRGSSTDVSTDPKVSIPPHDNLATKAMQNLIENMSPGRKGHVEMDQDGNNGGKSPYMGKKGSDVEDGGPGPRKQQMPPASVMTRLILIMVWRKLIRNPNTYSSLFGLAWSLVSFKWNIKMPTIMSGSISILSDAGLGMAMFSLGLFMALQPKIIACGKSVAGFAMAVRFLTGPAVIAATSIAIGIRGDLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLVALPVTVLYYVLLGL pid_arath Protein kinase PINOID Protein kinase ABRUPTUS T31E10.1 PID ABR At2g34650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLRESDGEMSLGTTNSPISSGTESCSSFSRLSFDAPPSTIPEEESFLSLKPHRSSDFAYAEIRRRKKQGLTFRDFRLMRRIGAGDIGTVYLCRLAGDEEESRSSYFAMKVVDKEALALKKKMHRAEMEKTILKMLDHPFLPTLYAEFEASHFSCIVMEYCSGGDLHSLRHRQPHRRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENILVRSDGHIMLSDFDLSLCSDSIAAVESSSSSPENQQLRSPRRFTRLARLFQRVLRSKKVQTLEPTRLFVAEPVTARSGSFVGTHEYVAPEVASGGSHGNAVDWWAFGVFLYEMIYGKTPFVAPTNDVILRNIVKRQLSFPTDSPATMFELHARNLISGLLNKDPTKRLGSRRGAAEVKVHPFFKGLNFALIRTLTPPEIPSSVVKKPMKSATFSGRSSNKPAAFDYF pp2aa1-pin1 Colocalisation of PP2AA1 protein and PIN1 protein 670697 N/A 670699 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 670700 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5F The authors state that "Quantitative analysis demonstrated that about 7% of the detected PP2AA1 signal colocalizes with PIN1, whereas 80% of PIN1 overlaps with PP2AA1". pp2aa1-pin2 Colocalisation of PP2AA1 protein and PIN2 protein 670697 N/A 670699 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 670701 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5C pid-pin2-1 Colocalisation of PID protein and PIN2 protein 670697 N/A 670702 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 670701 unspecified role unspecified role neutral component neutral component colocalization colocalization false false false 5I The authors note that colocalisation occured in the apical plasma membrane of epidermal cells and that a "quantitative analysis of colocalization revealed that 23% of PID:YFP colocalizes with PIN2, whereas 89% of PIN2 colocalizes with PID:YFP". pid-pin1 Phosphorylation of PIN1 protein by PID1 protein 670698 N/A 670700 enzyme target enzyme target substrate substrate neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 670702 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation false false false 7B phosphorylation of PIN1 protein.