MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint momose-2007-1 Structural basis of functional cooperation of Tim15/Zim17 with yeast mitochondrial Hsp70. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Momose T., Ohshima C., Maeda M., Endo T. EMBO Rep. (1469-221X) 2007 2009-01-08 Only protein-protein interactions imex curation hsp77_yeasx Import motor subunit, mitochondrial Heat shock protein SSC1, mitochondrial SSC1 ENS1 mtHSP70 Endonuclease SceI 75 kDa subunit protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MLAAKNILNRSSLSSSFRIATRLQSTKVQGSVIGIDLGTTNSAVAIMEGKVPKIIENAEGSRTTPSVVAFTKEGERLVGIPAKRQAVVNPENTLFATKRLIGRRFEDAEVQRDIKQVPYKIVKHSNGDAWVEARGQTYSPAQIGGFVLNKMKETAEAYLGKPVKNAVVTVPAYFNDSQRQATKDAGQIVGLNVLRVVNEPTAAALAYGLEKSDSKVVAVFDLGGGTFDISILDIDNGVFEVKSTNGDTHLGGEDFDIYLLREIVSRFKTETGIDLENDRMAIQRIREAAEKAKIELSSTVSTEINLPFITADASGPKHINMKFSRAQFETLTAPLVKRTVDPVKKALKDAGLSTSDISEVLLVGGMSRMPKVVETVKSLFGKDPSKAVNPDEAVAIGAAVQGAVLSGEVTDVLLLDVTPLSLGIETLGGVFTRLIPRNTTIPTKKSQIFSTAAAGQTSVEIRVFQGERELVRDNKLIGNFTLAGIPPAPKGVPQIEVTFDIDADGIINVSARDKATNKDSSITVAGSSGLSENEIEQMVNDAEKFKSQDEARKQAIETANKADQLANDTENSLKEFEGKVDKAEAQKVRDQITSLKELVARVQGGEEVNAEELKTKTEELQTSSMKLFEQLYKNDSNNNNNNNNGNNAESDETKQ mint Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed fratxin homolog YFH1 into the mitochondrion Acts as a non-catalytic component of endonuclease SceI (endo.SceI), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. SSC1 confers broader sequence specificity, greater stability, and higher activity on the catalytic subunit zim17_yeast Mitochondrial protein import protein ZIM17 TIM15 N0381 YNL310C FMP28 HEP1 mtHsp70-associated motor and chaperone protein TIM15/ZIM17 ZIM17 Mitochondrial import inner membrane translocase subunit TIM15 mtHsp70 escort protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIPRTRTLLQSKIPITRYFARCWAPRVRYNVCRTLPAAALHTNIIAHNEVKKDDKKVHLGSFKVDKPKMMIAFTCKKCNTRSSHTMSKQAYEKGTVLISCPHCKVRHLIADHLKIFHDHHVTVEQLMKANGEQVSQDVGDLEFEDIPDSLKDVLGKYAKNNSENASQLPHPSQK zim17-ssc1 664518 hsp77_yeast mtHsp70 664519 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified zim17_yeast Tim 15 664520 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P42844:p.Asp111Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false F4A F3F homomint mint zim17-ssc1-1 664518 hsp77_yeast mtHsp70 664519 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey purified purified zim17_yeast Tim 15 664520 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified P42844:p.Arg106Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P42844:p.His107Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false F4A F3F homomint mint