MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint vasudevan-2007-4 The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Vasudevan S., Starostina NG., Kipreos ET. ekipreos@cb.uga.edu EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation vasudevan-2007-1 The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. caeel-gavid adult Caenorhabditis elegans - pregnant adult gravid_adult pregnant adult anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Vasudevan S., Starostina NG., Kipreos ET. ekipreos@cb.uga.edu EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation vasudevan-2007-3 The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vasudevan S., Starostina NG., Kipreos ET. ekipreos@cb.uga.edu EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation vasudevan-2007-5 The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vasudevan S., Starostina NG., Kipreos ET. ekipreos@cb.uga.edu EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation vasudevan-2007-2 The Caenorhabditis elegans cell-cycle regulator ZYG-11 defines a conserved family of CUL-2 complex components. caeel-gavid adult Caenorhabditis elegans - pregnant adult gravid_adult pregnant adult anti tag coip anti tag coimmunoprecipitation anti tag coip polyclonal western polyclonal antibody western blot polyclonal western Vasudevan S., Starostina NG., Kipreos ET. ekipreos@cb.uga.edu EMBO Rep. (1469-221X) 2007 Only protein-protein interactions imex curation q9bks1_caeel Elongin-C elc-1 Y82E9BR.15 CELE_Y82E9BR.15 protein protein caeel Caenorhabditis elegans MADQNNAIQCDQDAAQPKQYGGIEGPTSQYVKLVSSDDHEFIIKRELALTSGTIRAMLSGPGVYAENESNVVYFREIPSHVLQKVCQYFAYKVRYTHAATEIPEFPIPPDVALELLMAANFLDC zyg11_caeel Early embryogenesis protein zyg-11 zyg-11 C08B11.1 protein protein caeel Caenorhabditis elegans MDHNAALTTCSTSAIPRLARLATERIAELIENDALPEFDHKVIASCSNDIFEVLRQKKKLNHQTLQTMCSTSRFQINKIDLMGNKVELQDLELCSNQNLVSFRLGDIDYFPNTNKIQVADVLDTALNRTSRQQLRHLDLSGRHRITSNWPTYVARKLPRLESLAFANRSTANETLSQIGASLLNLRFLDISSTCVTDISCISSLRNLEVLIMYNLNILKGDVTETLSNLTKLRVLDISRKVNTDYLQETSQDAHLDLALGIYNRSVEAIESGTATPWAELRAIDMSGLSIVQFGTDRALAFVEKIIEAHPKLEQISLLATPLDSSLIEIPNRNLQVINTVSRRSIIFALSHYANLDRPAFITHALHSVYYQLQSGYDKFSQEELKECLRLVCISMQQGLNTLPVQIAGSACLYHLCKMKRIKRLSVKEVNNCIERSLDAAEQYRSMTQLQKNVWLTICNDYLLHLDEIDFYRTCKVALDTMLLNRDASVERMTIAIVSIVTPKMRPSEAKILTTETKYVYHLVKIMNDYLEAYTREHRVGHERDNENALYTLKFTLSALWNLTDECPATCKAFLDAGGVQIAFRILKAFDYHGNVQTKVLGILNNLAEVEELHLGQLCKNEYISVLISCLDGSFNEVDSKGRYREVERSYFAAGILANLLTNTDGWESENQRDEACEKLLELIEQYPTLPSAMVSYKSFIPFSRIVRESNSNGAIMWCLWGVHHVLQHREKNKAPTYEKGYIEMFMDSGLSPIVEDMSHGHSKHIHNLDIRVVHLAREIIDIVSCHSLSSSPVRLVRRV vhl_caeel von Hippel-Lindau tumor suppressor homolog vhl-1 F08G12.4 protein protein caeel Caenorhabditis elegans MSDGSMDDDGRLFPDLGSSTHDNREIRVRFLNRCAYPVDVFWLNPSKQPTKYGTLAQKKYLDIKTFKDHPWVARRSFDGCKVLVNEKEVFWPEPAPRMNLIVRNHCVITMKVQSLREIAGRSFLRHNPTEVPNKIKGLPRELQFEVKHFLDRKQEYSEIVCRSIPPPGPQRPQQ zer1_caeel Zyg eleven-related protein 1 zer-1 zyg-11-related protein T24C4.6 protein protein caeel Caenorhabditis elegans MSHTADIPLEAPTLLKLASSSVQSQINNDEYMETVILPVPLSQELFARFRNKWNIHNHGPAPVEHLGPPTDEPRPDQGNIDMIKQVFRIWSDSTRLPLEKLNLTGATVDDQIFVDLLHAHAHSITELDLTNVTGVTDLSNAYLYARSTHFPMLTSIRMTSMDLVTGHQPRRKNGIASTEFRALFMLGEEALAEARGQMERDGLRSPLSPSSQPSSIQSDHQSLDALVPCDSPVEPPLITARAPNVKRLFLPRCPAKRTTIDEEEQNTHVILTKILSPLQQLEVLDLSYWSKTDDMRCLQPLSNTLTCLILYDVPDLYHAVPNICHMTELRILDVSQSNRDSGLYPHPVTTLNKLIVSLKYLTHLDISSTNLATQPSSHDNPARYRESVRTDICGLQSLVRPLKYLGLFNCESASHVREIPAEVVSGDANEDQVITSLQMYKDRAGLLQNVLNESYQLYRFGNSNPLTRHTEALHLVLEAMHRHLADSTLQIAGSASLFYIIRKVDMNRDTKRRVVSALLSGMEVHMEEQVMVRNCCLSLCQFEIPQDILFDYSRLAVLLVSVLQHHNADNLTQRIVVFLLNSMACHVEGDQKVQVGSYGAIEMILDQIARKHTANVCDDVMEVGWSFLWNITDETPVNCELFLNANGLDLFQKCYEAFKTERELVRNMMGLIGNIAEVDELRSQLMKDDYVKIFCALLESQEESIEISYNSAGVLAHMVSDGEEVWKQMTVCRNEVMQRIVEATSSWKLATRRFINYRSFRPILRLLPLYHAYASQHWAVWALANLTTTDGEKYCAYVRDEGGVPLLEELVSNAITTTDIRTLANTVLENIRNVENKRNPKSIPADLAHSSNSPTFVPSYVSDVEEEDEGDVEADEEVQDFVNVDFDDYALPMEY zy11b_human Protein zyg-11 homolog B ZYG11B KIAA1730 protein protein human Homo sapiens Human MPEDQAGAAMEEASPYSLLDICLNFLTTHLEKFCSARQDGTLCLQEPGVFPQEVADRLLRTMAFHGLLNDGTVGIFRGNQMRLKRACIRKAKISAVAFRKAFCHHKLVELDATGVNADITITDIISGLGSNKWIQQNLQCLVLNSLTLSLEDPYERCFSRLSGLRALSITNVLFYNEDLAEVASLPRLESLDISNTSITDITALLACKDRLKSLTMHHLKCLKMTTTQILDVVRELKHLNHLDISDDKQFTSDIALRLLEQKDILPNLVSLDVSGRKHVTDKAVEAFIQQRPSMQFVGLLATDAGYSEFLTGEGHLKVSGEANETQIAEALKRYSERAFFVREALFHLFSLTHVMEKTKPEILKLVVTGMRNHPMNLPVQLAASACVFNLTKQDLAAGMPVRLLADVTHLLLKAMEHFPNHQQLQKNCLLSLCSDRILQDVPFNRFEAAKLVMQWLCNHEDQNMQRMAVAIISILAAKLSTEQTAQLGTELFIVRQLLQIVKQKTNQNSVDTTLKFTLSALWNLTDESPTTCRHFIENQGLELFMRVLESFPTESSIQQKVLGLLNNIAEVQELHSELMWKDFIDHISSLLHSVEVEVSYFAAGIIAHLISRGEQAWTLSRSQRNSLLDDLHSAILKWPTPECEMVAYRSFNPFFPLLGCFTTPGVQLWAVWAMQHVCSKNPSRYCSMLIEEGGLQHLYNIKDHEHTDPHVQQIAVAILDSLEKHIVRHGRPPPCKKQPQARLN eloc_human Elongin-C ELOC Elongin 15 kDa subunit RNA polymerase II transcription factor SIII subunit C SIII p15 TCEB1 Transcription elongation factor B polypeptide 1 protein protein human Homo sapiens Human MDGEEKTYGGCEGPDAMYVKLISSDGHEFIVKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYKVRYTNSSTEIPEFPIAPEIALELLMAANFLDC zer1_human Protein zer-1 homolog Zyg-11 homolog B-like protein Hzyg ZER1 C9orf60 ZYG ZYG11BL Zyg11b-like protein protein protein human Homo sapiens Human MASDTPESLMALCTDFCLRNLDGTLGYLLDKETLRLHPDIFLPSEICDRLVNEYVELVNAACNFEPHESFFSLFSDPRSTRLTRIHLREDLVQDQDLEAIRKQDLVELYLTNCEKLSAKSLQTLRSFSHTLVSLSLFGCTNIFYEEENPGGCEDEYLVNPTCQVLVKDFTFEGFSRLRFLNLGRMIDWVPVESLLRPLNSLAALDLSGIQTSDAAFLTQWKDSLVSLVLYNMDLSDDHIRVIVQLHKLRHLDISRDRLSSYYKFKLTREVLSLFVQKLGNLMSLDISGHMILENCSISKMEEEAGQTSIEPSKSSIIPFRALKRPLQFLGLFENSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDRNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKTCDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGVCEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDIIKMATARQETKEMARKVIEHCSNFKEENMDTSR elob_human Elongin-B ELOB Elongin 18 kDa subunit RNA polymerase II transcription factor SIII subunit B SIII p18 TCEB2 Transcription elongation factor B polypeptide 2 protein protein human Homo sapiens Human MDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADDTFEALCIEPFSSPPELPDVMKPQDSGSSANEQAVQ cul2_caeel Cullin-2 cul-2 ccd-3 div-3 ZK520.4 protein protein caeel Caenorhabditis elegans MASSTKVSLRRPPRPQVMYSLKPKVVEFDKVWVQLRPSIIDIINLRPITNVQWHHKFSDVYDICVSIPTPLSERLYNEVKACIQEHVRQKRQDIVDVDPDLLLQEYHKMWRVFHEGAIFIHRLFGYLNKQFVKQKRCTDLDNFAQYAAFLQIPDVKEIGCLALEIWKEDLVKTILPQLVKLLLIAIDNDRKGNFPHIANEVSGVINSFVKMEETDFDVVPAEGARYKARESTTAFYQESFEKPLLTDTEQYYSALAQKMLTDLSCSEYMEQVIVLLEQEEMRAKKYLHESSVEKVITLCQKVMIKAHKDKLHAVCHDLITNEENKDLRNMYRLLKPIQAGLSVMVKEFEEYVKKKGLEAVSRLTGENVPQQFVENVLRVYNKFNDMKTAVFMDDGEFSSGLDKALQGVVNSKEPGQSVPKASERLARYTDGLLKKSTKGLSETDLEAKLDSAIVIFRYIEDKDIFQKFYSKMLANRLIASTSISMDAEELMINKLKQACGYEFTSKLSRMFTDIGLSQELSNNFDKHIADIKTVQPDVKFVPTQTMILQAGSWPLNAPQLSTNSNNQTAQDVANFHLPRILQPVIQEFEKFYTGKHNGRKLTWLFNMSQGDVRLTYLDKQYVAQMYVYQMAALLCFERRDAILVKDIGEEIGVSGDYLLKTIRTILDVTLLTCDDQNLTADSLVRLNMSMTSKRMKFRLQAPQVNKAVEKEQEAVANTVSQDRKYYMECAIVRIMKTRKVLKHNALVTEIMDQTKGRFSPDVPFIKKSIEDLIEKMYIQRTDQNDEYQYLA cul2_human Cullin-2 CUL2 protein protein human Homo sapiens Human MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA zyg11-elc1-1 598111 N/A 598116 unspecified role unspecified role prey prey N/A 598117 unspecified role unspecified role bait bait P21541:p.Leu21Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P21541:p.Leu18Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 2C, 3A, 3C vhl1-elc1-1 598111 N/A 598118 unspecified role unspecified role bait bait N/A-x-x-x 598116 unspecified role unspecified role prey prey physical association physical association false false false Fig 2C zer1-elc1-1 598111 N/A 598119 unspecified role unspecified role bait bait N/A-x 598116 unspecified role unspecified role prey prey physical association physical association false false false Fig 4B zyg11b-tceb1-1 598111 N/A 598120 unspecified role unspecified role bait bait Q9C0D3:p.Leu18Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q9C0D3:p.Cys22Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A 598121 unspecified role unspecified role prey prey physical association physical association false false false Fig 6A zer1-tceb1-1 598111 N/A 598122 unspecified role unspecified role bait bait Q7Z7L7:p.Leu9Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q7Z7L7:p.Cys13Ser mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A-x 598121 unspecified role unspecified role prey prey n/a 598123 unspecified role unspecified role ancillary ancillary physical association physical association false false false Fig 6A cul2-zyg11-1 598112 N/A 598124 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 598117 unspecified role unspecified role prey prey physical association physical association false false false cul2-zer1-1 598112 N/A-x 598124 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 598119 unspecified role unspecified role prey prey physical association physical association false false false Fig 4A elc1-cul2-1 598113 N/A 598124 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 598116 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 2B elc1-zyg11-1 598113 N/A 598117 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 598116 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Fig 2B zyg11b-cul2-1 598114 N/A 598120 unspecified role unspecified role bait bait c-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 598125 unspecified role unspecified role prey prey physical association physical association false false false Fig 6B zer1-cul2-1 598114 N/A 598122 unspecified role unspecified role bait bait c-terminus vsv tag vsv tag vesicular stomatitis virus tag vesicular stomatitis virus tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A-x 598125 unspecified role unspecified role prey prey physical association physical association false false false Fig 6B cul2-zyg11-2 598115 N/A 598124 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 598117 unspecified role unspecified role prey prey physical association physical association false false false Fig 2A Interaction between cul-2 and zyg-11 is inhibited by RNAi against elc-1.