IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ di_fulvio-2007-1 The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation di_fulvio-2007-2 The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. human-uvec Human Umbilical Vein Endothelial Cells (HUVEC) huvec umbilical_vein_endothelium Umbilical vein endothelial cell [Umbilical vein endothelium] fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation di_fulvio-2007-3 The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation di_fulvio-2007-4 The Grb2/PLD2 interaction is essential for lipase activity, intracellular localization and signaling in response to EGF. chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Di Fulvio M., Frondorf K., Henkels KM., Lehman N., Gomez-Cambronero J. julian.cambronero@wright.edu 2007-07-17: Julian Gomez-Cambronero, Cell Biology and Physiology, Wright State University, School of Medicine, Dayton, OH, 45435, USA J. Mol. Biol. (0022-2836) 2007 Only protein-protein interactions imex curation pld2_human Phospholipase D2 Choline phosphatase 2 Phosphatidylcholine-hydrolyzing phospholipase D2 PLD1C PLD2 protein protein human Homo sapiens Human MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRAGPEGSTRHAASKQKYLENYLNRLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVADAILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFIQRWNFTKTTKAKYKTPTYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQAILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT grb2_human Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 SH2/SH3 adapter GRB2 Protein Ash protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV sos_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt grb2_cerae_protein Cercopithecus aethiops Grb2 protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P62993 pld2-grb2-1 659342 N/A 659346 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659347 unspecified role unspecified role prey prey physical association physical association false false false 5 pld2-sos 659342 N/A 659348 unspecified role unspecified role prey prey N/A 659346 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 4a pld2-grb2-2 659343 N/A 659346 unspecified role unspecified role neutral component neutral component N/A 659347 unspecified role unspecified role neutral component neutral component physical association physical association false false false 8 EGF pld2-grb2-3 659344 N/A 659346 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false tyr169 phe178 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 659347 unspecified role unspecified role neutral component neutral component P62993:p.Arg86Lys mutation mutation certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 6, 7 EGF pld2-grb2-4 659345 N/A 659346 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 659349 unspecified role unspecified role bait bait physical association physical association false false false 4a