DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ singh-2006a-2 Dissecting virulence pathways of Mycobacterium tuberculosis through protein-protein association. mycsm Mycobacterium smegmatis dhfr reconstruction dihydrofolate reductase reconstruction dhfr reconstruction nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Singh A., Mai D., Kumar A., Steyn AJ. Transformants were selected on 7H11 medium supplemented with kanamycin (KAN) and hygromycin (HYG; 7H11/KAN/HYG). Transformants were then subcultured onto 7H11KAN/HYG plates containing 50 ug/ml TRIM (7H11/TRIM) or freshly inoculated into 96-well microtiter plates containing 7H9/KAN/HYG/TRIM. A change from nonfluorescent blue to fluorescent pink color indicates reduction of the indicator Alamar Blue and allowed quantification of the assay. asteyn@uab.edu Roland Krause, MPI, Germany Mycobacterium tuberculosis H37Rv genomic DNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Only protein-protein interactions singh-2006a-1 Dissecting virulence pathways of Mycobacterium tuberculosis through protein-protein association. mycsm Mycobacterium smegmatis dhfr reconstruction dihydrofolate reductase reconstruction dhfr reconstruction predetermined predetermined participant predetermined Singh A., Mai D., Kumar A., Steyn AJ. Transformants were selected on 7H11 medium supplemented with kanamycin (KAN) and hygromycin (HYG; 7H11/KAN/HYG). Transformants were then subcultured onto 7H11KAN/HYG plates containing 50 ug/ml TRIM (7H11/TRIM) or freshly inoculated into 96-well microtiter plates containing 7H9/KAN/HYG/TRIM. A change from nonfluorescent blue to fluorescent pink color indicates reduction of the indicator Alamar Blue and allowed quantification of the assay. asteyn@uab.edu Roland Krause, MPI, Germany Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Only protein-protein interactions singh-2006a-3 Dissecting virulence pathways of Mycobacterium tuberculosis through protein-protein association. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Singh A., Mai D., Kumar A., Steyn AJ. Transformants were selected on 7H11 medium supplemented with kanamycin (KAN) and hygromycin (HYG; 7H11/KAN/HYG). Transformants were then subcultured onto 7H11KAN/HYG plates containing 50 ug/ml TRIM (7H11/TRIM) or freshly inoculated into 96-well microtiter plates containing 7H9/KAN/HYG/TRIM. A change from nonfluorescent blue to fluorescent pink color indicates reduction of the indicator Alamar Blue and allowed quantification of the assay. asteyn@uab.edu Roland Krause, MPI, Germany Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Only protein-protein interactions singh-2006a-4 Dissecting virulence pathways of Mycobacterium tuberculosis through protein-protein association. ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Singh A., Mai D., Kumar A., Steyn AJ. Transformants were selected on 7H11 medium supplemented with kanamycin (KAN) and hygromycin (HYG; 7H11/KAN/HYG). Transformants were then subcultured onto 7H11KAN/HYG plates containing 50 ug/ml TRIM (7H11/TRIM) or freshly inoculated into 96-well microtiter plates containing 7H9/KAN/HYG/TRIM. A change from nonfluorescent blue to fluorescent pink color indicates reduction of the indicator Alamar Blue and allowed quantification of the assay. asteyn@uab.edu Roland Krause, MPI, Germany Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2006 imex curation Only protein-protein interactions esxb_myctu ESAT-6-like protein EsxB esxB cfp10 lhp mtsA10 Rv3874 MTV027.09 10 kDa culture filtrate antigen CFP-10 Secreted antigenic protein MTSA-10 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MAEMKTDAATLAQEAGNFERISGDLKTQIDQVESTAGSLQGQWRGAAGTAAQAAVVRFQEAANKQKQELDEISTNIRQAGVQYSRADEEQQQALSSQMGF esxa_myctu 6 kDa early secretory antigenic target esxA esaT6 Rv3875 MTV027.10 ESAT-6 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA i6xvz6_myctu Rv0686 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MLARYIKMQLLVLLCGGLVGPIFLVVYFTLGLGSLMSWMFYVGLIITVADVLVALALTNYGAKTAAKTAALERSGVLALAQITGLSETGTRINDQPLVKVHLHISGPGITPFDTEDRVIASVTRLGNLTARKLVVLVNPATQQYLIDWERSALVNGLVPAQFTVAEDNKTYDLSGQTGPLMEILQILKANNVPLNRMVDIRSNPALRQQVQAVVRRAAERQAPAAEPASQGSIAERLAELESLRASGAVNAAEYESKRAQIISEI ftsq_myctu Cell division protein FtsQ ftsQ Rv2151c MTCY270.17 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTEHNEDPQIERVADDAADEEAVTEPLATESKDEPAEHPEFEGPRRRARRERAERRAAQARATAIEQARRAAKRRARGQIVSEQNPAKPAARGVVRGLKALLATVVLAVVGIGLGLALYFTPAMSAREIVIIGIGAVSREEVLDAARVRPATPLLQIDTQQVADRVATIRRVASARVQRQYPSALRITIVERVPVVVKDFSDGPHLFDRDGVDFATDPPPPALPYFDVDNPGPSDPTTKAALQVLTALHPEVASQVGRIAAPSVASITLTLADGRVVIWGTTDRCEEKAEKLAALLTQPGRTYDVSSPDLPTVK clpc1_myctu ATP-dependent Clp protease ATP-binding subunit ClpC1 clpC1 MTCY07H7B.26 Rv3596c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGVAAKSLESLGISLEGVRSQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNYIGTEHILLGLIREGEGVAAQVLVKLGAELTRVRQQVIQLLSGYQGKEAAEAGTGGRGGESGSPSTSLVLDQFGRNLTAAAMEGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGKTAVVEGLAQAIVHGEVPETLKDKQLYTLDLGSLVAGSRYRGDFEERLKKVLKEINTRGDIILFIDELHTLVGAGAAEGAIDAASILKPKLARGELQTIGATTLDEYRKYIEKDAALERRFQPVQVGEPTVEHTIEILKGLRDRYEAHHRVSITDAAMVAAATLADRYINDRFLPDKAIDLIDEAGARMRIRRMTAPPDLREFDEKIAEARREKESAIDAQDFEKAASLRDREKTLVAQRAEREKQWRSGDLDVVAEVDDEQIAEVLGNWTGIPVFKLTEAETTRLLRMEEELHKRIIGQEDAVKAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDALIQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKPFSVVLFDEIEKAHQEIYNSLLQVLEDGRLTDGQGRTVDFKNTVLIFTSNLGTSDISKPVGLGFSKGGGENDYERMKQKVNDELKKHFRPEFLNRIDDIIVFHQLTREEIIRMVDLMISRVAGQLKSKDMALVLTDAAKALLAKRGFDPVLGARPLRRTIQREIEDQLSEKILFEEVGPGQVVTVDVDNWDGEGPGEDAVFTFTGTRKPPAEPDLAKAGAHSAGGPEPAAR pks13_myctu Polyketide synthase Pks13 pks13 Rv3800c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MADVAESQENAPAERAELTVPEMRQWLRNWVGKAVGKAPDSIDESVPMVELGLSSRDAVAMAADIEDLTGVTLSVAVAFAHPTIESLATRIIEGEPETDLAGDDAEDWSRTGPAERVDIAIVGLSTRFPGEMNTPEQTWQALLEGRDGITDLPDGRWSEFLEEPRLAARVAGARTRGGYLKDIKGFDSEFFAVAKTEADNIDPQQRMALELTWEALEHARIPASSLRGQAVGVYIGSSTNDYSFLAVSDPTVAHPYAITGTSSSIIANRVSYFYDFHGPSVTIDTACSSSLVAIHQGVQALRNGEADVVVAGGVNALITPMVTLGFDEIGAVLAPDGRIKSFSADADGYTRSEGGGMLVLKRVDDARRDGDAILAVIAGSAVNHDGRSNGLIAPNQDAQADVLRRAYKDAGIDPRTVDYIEAHGTGTILGDPIEAEALGRVVGRGRPADRPALLGAVKTNVGHLESAAGAASMAKVVLALQHDKLPPSINFAGPSPYIDFDAMRLKMITTPTDWPRYGGYALAGVSSFGFGGANAHVVVREVLPRDVVEKEPEPEPEPKAAAEPAEAPTLAGHALRFDEFGNIITDSAVAEEPEPELPGVTEEALRLKEAALEELAAQEVTAPLVPLAVSAFLTSRKKAAAAELADWMQSPEGQASSLESIGRSLSRRNHGRSRAVVLAHDHDEAIKGLRAVAAGKQAPNVFSVDGPVTTGPVWVLAGFGAQHRKMGKSLYLRNEVFAAWIEKVDALVQDELGYSVLELILDDAQDYGIETTQVTIFAIQIALGELLRHHGAKPAAVIGQSLGEAASAYFAGGLSLRDATRAICSRSHLMGEGEAMLFGEYIRLMALVEYSADEIREVFSDFPDLEVCVYAAPTQTVIGGPPEQVDAILARAEAEGKFARKFATKGASHTSQMDPLLGELTAELQGIKPTSPTCGIFSTVHEGRYIKPGGEPIHDVEYWKKGLRHSVYFTHGIRNAVDSGHTTFLELAPNPVALMQVALTTADAGLHDAQLIPTLARKQDEVSSMVSTMAQLYVYGHDLDIRTLFSRASGPQDYANIPPTRFKRKEHWLPAHFSGDGSTYMPGTHVALPDGRHVWEYAPRDGNVDLAALVRAAAAHVLPDAQLTAAEQRAVPGDGARLVTTMTRHPGGASVQVHARIDESFTLVYDALVSRAGSESVLPTAVGAATAIAVADGAPVAPETPAEDADAETLSDSLTTRYMPSGMTRWSPDSGETIAERLGLIVGSAMGYEPEDLPWEVPLIELGLDSLMAVRIKNRVEYDFDLPPIQLTAVRDANLYNVEKLIEYAVEHRDEVQQLHEHQKTQTAEEIARAQAELLHGKVGKTEPVDSEAGVALPSPQNGEQPNPTGPALNVDVPPRDAAERVTFATWAIVTGKSPGGIFNELPRLDDEAAAKIAQRLSERAEGPITAEDVLTSSNIEALADKVRTYLEAGQIDGFVRTLRARPEAGGKVPVFVFHPAGGSTVVYEPLLGRLPADTPMYGFERVEGSIEERAQQYVPKLIEMQGDGPYVLVGWSLGGVLAYACAIGLRRLGKDVRFVGLIDAVRAGEEIPQTKEEIRKRWDRYAAFAEKTFNVTIPAIPYEQLEELDDEGQVRFVLDAVSQSGVQIPAGIIEHQRTSYLDNRAIDTAQIQPYDGHVTLYMADRYHDDAIMFEPRYAVRQPDGGWGEYVSDLEVVPIGGEHIQAIDEPIIAKVGEHMSRALGQIEADRTSEVGKQ y2240_myctu Uncharacterized protein Rv2240c Rv2240c MTCY427.21c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MLIGWRAVPRRHGGELPRRGALALGCIALLLMGIVGCTTVTDGTAMPDTNVAPAYRSSVSASVSASAATSSIRESQRQQSLTTKAIRTSCDALAATSKDAIDKVNAYVAAFNQGRNTGPTEGPAIDALNNSASTVSGSLSAALSAQLGDALNAYVDAARAVANAIGAHASTAEFNRRVDRLNDTKTKALTMCVAAF gcn4_yeast General control transcription factor GCN4 ARG9 GCN4 AAS3 Amino acid biosynthesis regulatory protein AAS101 General control protein GCN4 YEL009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER 2ED1B8E35D509578 kdpd_myctu Sensor protein KdpD kdpD Rv1028c MTCY10G2.21 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTLLFADLCAIFTPYRWMIEHVTTKRGQLRIYLGAAPGVGKTYAMLGEAHRRLERGTDVVAAVVETHGRNKTAKLLEGIEMIPPRYVEYRGARFPELDVEAVLRRHPQVVLVDELAHTNTPGSKNPKRWQDVQEILDAGITVISTVNIQHLEGLNDVVEQITGIEQKEKIPDEIVRAADQVELVDITPEALRRRLAHGNVYAAERVDAALSNYFRTGNLTALREIALLWLADQVDAALEKYRADKKITATWEARERVVVAVTGGPESETLVRRASRIASKSSAELMVVHVIRGDGLAGVSAPQLGRVRELATSLGATMHTVVGDDVPTALLDFAREMNATQLVVGTSRRSRWARLFDEGIGARTVQEPGGIDVHMVTHPAASRASGWSRVSPRERHIASWLAALVVPSVICAITVAWLDRFMGIGGESALFFIGVLIVALLGGVAPAALSALLSGMLLNYFLTEPRYTWTIAEPDAAVTEFVLLAMAVAVAVLVDGAASRTREARRASQEAELLALFAGSVLRGADLATLLQRVRETYSQRAVTMLRVRQGASTGETVACVGTNPCRDVDSADTAIEVGDDEFWMLMAGRKLAARDRRVLTAVATQAAGLVKQRELAEEAGQAEAIARADELRRSLLSAVSHDLRTPLAAAKVAVSSLRTEDVAFSPEDTAELLATIEESIDQLTALVANLLDSSRLAAGVIRPQLRRAYLEEAVQRALVSIGKGATGFYRSGIDRVKVDVGDAVAMADAGLLERVLANLIDNALRYAPDCVVRVNAGRVRERVLINVIDEGPGVPRGTEEQLFAPFQRPGDHDNTTGVGLGMSVARGFVEAMGGTISATDTPGGGLTVVIDLAAPEDRP kdpe_myctu Transcriptional regulatory protein KdpE kdpE Rv1027c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTLVLVIDDEPQILRALRINLTVRGYQVITASTGAGALRAAAEHPPDVVILDLGLPDMSGIDVLGGLRGWLTAPVIVLSARTDSSDKVQALDAGADDYVTKPFGMDEFLARLRAAVRRNTAAAELEQPVIETDSFTVDLAGKKVIKDGAEVHLTPTEWGMLEMLARNRGKLVGRGELLKEVWGPAYATETHYLRVYLAQLRRKLEDDPSHPKHLLTESGMGYRFEA devr_myctu DNA-binding transcriptional activator DevR/DosR devR dosR Rv3133c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MVKVFLVDDHEVVRRGLVDLLGADPELDVVGEAGSVAEAMARVPAARPDVAVLDVRLPDGNGIELCRDLLSRMPDLRCLILTSYTSDEAMLDAILAGASGYVVKDIKGMELARAVKDVGAGRSLLDNRAAAALMAKLRGAAEKQDPLSGLTDQERTLLGLLSEGLTNKQIADRMFLAEKTVKNYVSRLLAKLGMERRTQAAVFATELKRSRPPGDGP devs_myctu Oxygen sensor histidine kinase response regulator DevS/DosS devS dosS Rv3132c protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MTTGGLVDENDGAAMRPLRHTLSQLRLHELLVEVQDRVEQIVEGRDRLDGLVEAMLVVTAGLDLEATLRAIVHSATSLVDARYGAMEVHDRQHRVLHFVYEGIDEETVRRIGHLPKGLGVIGLLIEDPKPLRLDDVSAHPASIGFPPYHPPMRTFLGVPVRVRDESFGTLYLTDKTNGQPFSDDDEVLVQALAAAAGIAVANARLYQQAKARQSWIEATRDIATELLSGTEPATVFRLVAAEALKLTAADAALVAVPVDEDMPAADVGELLVIETVGSAVASIVGRTIPVAGAVLREVFVNGIPRRVDRVDLEGLDELADAGPALLLPLRARGTVAGVVVVLSQGGPGAFTDEQLEMMAAFADQAALAWQLATSQRRMRELDVLTDRDRIARDLHDHVIQRLFAIGLALQGAVPHERNPEVQQRLSDVVDDLQDVIQEIRTTIYDLHGASQGITRLRQRIDAAVAQFADSGLRTSVQFVGPLSVVDSALADQAEAVVREAVSNAVRHAKASTLTVRVKVDDDLCIEVTDNGRGLPDEFTGSGLTNLRQRAEQAGGEFTLASVPGASGTVLRWSAPLSQ p95032_mycto MT0714 protein protein mycto Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) MLARYIKMQLLVLLCGGLVGPIFLVVYFTLGLGSLMSWMFYVGLIITVADVLVALALTNYGAKTAAKTAALERSGVLALAQITGLSETGTRINDQPLVKVHLHISGPGITPFDTEDRVIASVTRLGNLTARKLVVLVNPATQQYLIDWERSALVNGLVPAQFTVAEDNKTYDLSGQTGPLMEILQILKANNVPLNRMVDIRSNPALRQQVQAVVRRAAERQAPAAEPASQGSIAERLAELESLRASGAVNAAEYESKRAQIISEI clpp2_myctu ATP-dependent Clp protease proteolytic subunit 2 clpP2 Rv2460c MTV008.16c Endopeptidase Clp 2 protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MNSQNSQIQPQARYILPSFIEHSSFGVKESNPYNKLFEERIIFLGVQVDDASANDIMAQLLVLESLDPDRDITMYINSPGGGFTSLMAIYDTMQYVRADIQTVCLGQAASAAAVLLAAGTPGKRMALPNARVLIHQPSLSGVIQGQFSDLEIQAAEIERMRTLMETTLARHTGKDAGVIRKDTDRDKILTAEEAKDYGIIDTVLEYRKLSAQTA esxa-esxb-3 Interaction between EsxA and EsxB identified by DHFR 608681 N/A 608685 unspecified role unspecified role bait bait N/A 608686 unspecified role unspecified role prey prey physical association physical association false false false Table 1 rv0686-esxb-1 Interaction between Rv0686 and EsxB identified by DHFR 608681 N/A 608687 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Table 1 ftsq-esxb-1 Interaction between FtsQ and EsxB identified by DHFR 608681 N/A 608688 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Table 1 clpc-esxb-2 Interaction between ClpC and EsxB identified by DHFR 608681 N/A 608689 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Table 1 pks13-esxb-1 Interaction between Pks13 and EsxB identified by DHFR 608681 N/A 608685 unspecified role unspecified role bait bait N/A 608690 unspecified role unspecified role prey prey physical association physical association false false false Table 1 rv2240c-esxb-1 Interaction between Rv2240c and EsxB identified by DHFR 608681 N/A 608685 unspecified role unspecified role bait bait N/A 608691 unspecified role unspecified role prey prey physical association physical association false false false Table 1 gcn4-gcn4-1 Homodimerisation of GCN4 identified by DHFR 608682 N/A 608692 unspecified role unspecified role bait bait c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 608692 unspecified role unspecified role prey prey c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 3, 5 kdpe-kdpd-1 Interaction between KdpD and KdpE identified by DHFR 608682 N/A 608693 unspecified role unspecified role prey prey N/A 608694 unspecified role unspecified role bait bait physical association physical association false false false Fig. 3 esxa-esxb-1 Interaction between EsxA and EsxB identified by DHFR 608682 N/A 608686 unspecified role unspecified role bait bait c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 608685 unspecified role unspecified role prey prey c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 3, 4B, 5 esxa-esxb-2 Interaction between EsxA and EsxB identified by DHFR 608682 N/A 608686 unspecified role unspecified role bait bait n-terminal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 608685 unspecified role unspecified role prey prey n-terminal tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4B devs-devr-1 Interaction between DevS and DevR identified by DHFR 608682 N/A 608695 unspecified role unspecified role prey prey c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 608696 unspecified role unspecified role bait bait c-terminal tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 4B, 5 clpc-esxb-3 Interaction between ClpC and EsxB identified by Y2H 608683 N/A 608689 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Sup. Fig. 7 rv0686-esxb-2 Interaction between Rv0686 and EsxB identified by Y2H 608683 N/A 608685 unspecified role unspecified role bait bait N/A 608697 unspecified role unspecified role prey prey physical association physical association false false false Sup. Fig. 7 ftsq-esxb-2 Interaction between FtsQ and EsxB identified by Y2H 608683 N/A 608688 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Fig 7 rv2240c-esxb-2 Interaction between Rv2240c and EsxB identified by Y2H 608683 N/A 608691 unspecified role unspecified role prey prey N/A 608685 unspecified role unspecified role bait bait physical association physical association false false false Sup Fig 7 clpc-esxb-4 Interaction between ClpC and EsxB identified by pulldown 608684 N/A 608689 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 608685 unspecified role unspecified role prey prey physical association physical association false false false Sup. Fig. 8 clpc-esxa-1 Interaction between ClpC and EsxA identified by pulldown 608684 N/A 608689 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 608686 unspecified role unspecified role prey prey physical association physical association false false false Sup. Fig. 8 clpc-clpp2-1 Interaction between ClpC and clpp2 identified by pulldown 608684 N/A 608689 unspecified role unspecified role bait bait n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 608698 unspecified role unspecified role prey prey physical association physical association false false false Sup. Fig. 8