IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ chen-2005d-1 TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L. Author refers to protein as TSSK5, whereas it identifies as TSSK4 longyu@fudan.edu.cn Human testes cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2005 Only protein-protein interactions imex curation chen-2005d-2 TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L. Author refers to protein as TSSK5, whereas it identifies as TSSK4 longyu@fudan.edu.cn Human testes cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2005 Only protein-protein interactions imex curation chen-2005d-3 TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L. Author refers to protein as TSSK5, whereas it identifies as TSSK4 longyu@fudan.edu.cn Human testes cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2005 Only protein-protein interactions imex curation chen-2005d-4 TSSK5, a novel member of the testis-specific serine/threonine kinase family, phosphorylates CREB at Ser-133, and stimulates the CRE/CREB responsive pathway. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Chen X., Lin G., Wei Y., Hexige S., Niu Y., Liu L., Yang C., Yu L. Author refers to protein as TSSK5, whereas it identifies as TSSK4 longyu@fudan.edu.cn Human testes cDNA Biochem. Biophys. Res. Commun. (0006-291X) 2005 Only protein-protein interactions imex curation creb1_human Cyclic AMP-responsive element-binding protein 1 CREB1 protein protein human Homo sapiens Human MTMESGAENQQSGDAAVTEAENQQMTVQAQPQIATLAQVSMPAAHATSSAPTVTLVQLPNGQTVQVHGVIQAAQPSVIQSPQVQTVQISTIAESEDSQESVDSVTDSQKRREILSRRPSYRKILNDLSSDAPGVPRIEEEKSEEETSAPAITTVTVPTPIYQTSSGQYIAITQGGAIQLANNGTDGVQGLQTLTMTNAAATQPGTTILQYAQTTDGQQILVPSNQVVVQAASGDVQTYQIRTAPTSTIAPGVVMASSPALPTQPAEEAARKREVRLMKNREAARECRRKKKEYVKCLENRVAVLENQNKTLIEELKALKDLYCHKSD tssk4_human Testis-specific serine/threonine-protein kinase 4 TSSK4 C14orf20 STK22E TSSK5 Serine/threonine-protein kinase 22E protein protein human Homo sapiens Human MGKGDVLEAAPTTTAYHSLMDEYGYEVGKAIGHGSYGSVYEAFYTKQKVMVAVKIISKKKASDDYLNKFLPREIQVMKVLRHKYLINFYRAIESTSRVYIILELAQGGDVLEWIQRYGACSEPLAGKWFSQLTLGIAYLHSKSIVHRDLKLENLLLDKWENVKISDFGFAKMVPSNQPVGCSPSYRQVNCFSHLSQTYCGSFAYACPEILRGLPYNPFLSDTWSMGVILYTLVVAHLPFDDTNLKKLLRETQKEVTFPANHTISQECKNLILQMLRQATKRATILDIIKDSWVLKFQPEQPTHEIRLLEAMCQLHNTTKQHQSLQITT tssk4-creb1-1 Interaction between TSSK4 and CREB1 identified by Y2H 593911 N/A 593915 unspecified role unspecified role prey prey kid region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 593916 unspecified role unspecified role bait bait physical association physical association false false false 3A tssk4-creb1-2 Interaction between TSSK4 and CREB1 identified by Y2H 593912 N/A 593916 unspecified role unspecified role bait bait N/A 593915 unspecified role unspecified role prey prey kid region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3B tssk4-creb1-3 Interaction between TSSK4 and CREB1 identified by GST pulldown 593913 N/A 593916 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 593915 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association false false false 3C tssk4-creb1-4 Phosphorylation of CREB1 by TSSK4 593914 N/A 593916 enzyme enzyme unspecified role unspecified role region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. N/A 593915 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation false false false 4 and 5 Phosphorylation of Ser-133 (mutational analysis)