IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ li-2005d-3 The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7. in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay western blot western blot Immuno blot Immuno blot Li H., Sanchez-Torres J., del Carpio AF., Nogales-Gonzalez A., Molina-Ortiz P., Moreno MJ., Torok K., Villalobo A. Immobilized phosphoinositides provided by the PIP Strips system Origin of P62157 taken from manufacturer's website antonio.villalobo@iib.uam.es 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation li-2005d-1 The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li H., Sanchez-Torres J., del Carpio AF., Nogales-Gonzalez A., Molina-Ortiz P., Moreno MJ., Torok K., Villalobo A. Origin of P62157 taken from manufacturer's website antonio.villalobo@iib.uam.es 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation li-2005d-2 The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Li H., Sanchez-Torres J., del Carpio AF., Nogales-Gonzalez A., Molina-Ortiz P., Moreno MJ., Torok K., Villalobo A. Origin of P62157 taken from manufacturer's website antonio.villalobo@iib.uam.es 2007-06-01: Antonio Villalobo, Instituto de Investigaciones Biomedicas, Universidad Autonoma de Madrid, Spain Oncogene (0950-9232) 2005 Only protein-protein interactions imex curation calm_rat Calmodulin Calm1 Calm Cam Cam1 Calm2 Cam2 Camb Calm3 Cam3 Camc CaMII protein protein rat Rattus norvegicus (Rat) Rat MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) grb7_human Growth factor receptor-bound protein 7 GRB7 GRB7 adapter protein Epidermal growth factor receptor GRB-7 B47 protein protein human Homo sapiens Human MELDLSPPHLSSSPEDLCPAPGTPPGTPRPPDTPLPEEVKRSQPLLIPTTGRKLREEERRATSLPSIPNPFPELCSPPSQSPILGGPSSARGLLPRDASRPHVVKVYSEDGACRSVEVAAGATARHVCEMLVQRAHALSDETWGLVECHPHLALERGLEDHESVVEVQAAWPVGGDSRFVFRKNFAKYELFKSSPHSLFPEKMVSSCLDAHTGISHEDLIQNFLNAGSFPEIQGFLQLRGSGRKLWKRFFCFLRRSGLYYSTKGTSKDPRHLQYVADVNESNVYVVTQGRKLYGMPTDFGFCVKPNKLRNGHKGLRIFCSEDEQSRTCWLAAFRLFKYGVQLYKNYQQAQSRHLHPSCLGSPPLRSASDNTLVAMDFSGHAGRVIENPREALSVALEEAQAWRKKTNHRLSLPMPASGTSLSAAIHRTQLWFHGRISREESQRLIGQQGLVDGLFLVRESQRNPQGFVLSLCHLQKVKHYLILPSEEEGRLYFSMDDGQTRFTDLLQLVEFHQLNRGILPCLLRHCCTRVAL pi5p 1-phosphatidyl-1D-myo-inositol 5-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,4-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,4,5-trisphosphate 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) phosphatidic acids phosphatidic acids small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK grb7-pi3p-1 Binding of 1-phosphatidyl-1D-myo-inositol 3-phosphate to Grb7 650875 n/a 650878 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) N/A 650879 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association false false false Fig 7 Calmodulin grb7-pi4p-1 Binding of 1-phosphatidyl-1D-myo-inositol 4-phosphate to Grb7 650875 n/a 650881 unspecified role unspecified role prey prey n/a 650878 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association false false false Fig 7 Calmodulin grb7-pi5p-1 Binding of 1-phosphatidyl-1D-myo-inositol 5-phosphate to Grb7 650875 n/a 650878 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 650881 unspecified role unspecified role prey prey association association false false false Fig 7 Calmodulin grb7-pi34p2-1 Binding of 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate to Grb7 650875 n/a 650882 unspecified role unspecified role prey prey n/a 650878 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association false false false Fig 7 Calmodulin grb7-pi35p2-1 Binding of 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate to Grb7 650875 n/a 650878 competitor competitor competitor prey prey ecoli Escherichia coli (strain K12) N/A 650883 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association false false false Fig 7 Calmodulin grb7-pi45p2-1 Binding of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to Grb7 650875 n/a 650878 competitor competitor competitor prey prey n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 650884 unspecified role unspecified role prey prey association association false false false Fig 7 Calmodulin grb7-pi345p2-1 Binding of 1-phosphatidyl-1D-myo-inositol 3,4,5-bisphosphate to Grb7 650875 N/A 650878 inhibitor inhibitor prey prey ecoli Escherichia coli (strain K12) N/A 650885 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association false false false Fig 7 Calmodulin grb7-pa-1 Binding of phosphatidic acid to Grb7 650875 n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 650886 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig 7 grb7-pi3p-2 Binding of 1-phosphatidyl-1D-myo-inositol 3-phosphate to Grb7 650875 N/A 650879 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false Fig 7 grb7-pi4p-2 Binding of 1-phosphatidyl-1D-myo-inositol 4-phosphate to Grb7 650875 n/a 650881 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false Fig 7 grb7-pi5p-2 Binding of 1-phosphatidyl-1D-myo-inositol 5-phosphate to Grb7 650875 n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 650881 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig 7 grb7-pi34p2-2 Binding of 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate to Grb7 650875 n/a 650882 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false Fig 7 grb7-pi35p2-2 Binding of 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate to Grb7 650875 N/A 650883 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false Fig 7 grb7-pi45p2-2 Binding of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to Grb7 650875 n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. N/A 650884 unspecified role unspecified role prey prey direct interaction direct interaction false false false Fig 7 grb7-pi345p2-2 Binding of 1-phosphatidyl-1D-myo-inositol 3,4,5-bisphosphate to Grb7 650875 N/A 650885 unspecified role unspecified role prey prey n/a 650880 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false Fig 7 calm-grb7-1 Binding of Grb7 to calmodulin agarose 650876 n/a 650880 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion N/A 650887 unspecified role unspecified role bait bait bovin-brain Bos taurus brain brain Brain [cerebrum] physical association physical association false false false Figure 1a Interaction is calcium dependent. calm-grb7-2 Binding of Grb7 to calmodulin agarose 650876 N/A 650887 unspecified role unspecified role bait bait bovin-brain Bos taurus brain brain Brain [cerebrum] n/a 650880 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 1b calm-grb7-3 Binding of Grb7 to calmodulin demonstrated by Far Western 650877 N/A 650878 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 650880 unspecified role unspecified role prey prey human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion physical association physical association false false false Figure 2b Interaction is calcium dependent calm-grb7-4 Binding of Grb7 to calmodulin demonstrated by Far Western 650877 n/a 650880 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 650878 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Figures 2 & 4 Interaction is calcium dependent