IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ basu-2004-2 Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Basu D., El-Assal Sel-D., Le J., Mallery EL., Szymanski DB. Corrigendum: In this article, the authors reported that complementation tests between pirogi and klunker plants proved that the two genes were distinct. They have subsequently learned that the klunker stocks were mislabeled, and that KLUNKER and PIROGI correspond to the same gene and encode a SRA1 homologue. dszyman@purdue.edu Development (0950-1991) 2004 basu-2004-1 Interchangeable functions of Arabidopsis PIROGI and the human WAVE complex subunit SRA1 during leaf epidermal development. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Basu D., El-Assal Sel-D., Le J., Mallery EL., Szymanski DB. Corrigendum: In this article, the authors reported that complementation tests between pirogi and klunker plants proved that the two genes were distinct. They have subsequently learned that the klunker stocks were mislabeled, and that KLUNKER and PIROGI correspond to the same gene and encode a SRA1 homologue. dszyman@purdue.edu Development (0950-1991) 2004 pir_arath Protein PIR PIR of plants Protein PIROGI Protein KLUNKER PIR KLK PIRP F20L16.130 At5g18410 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAVPVEEAIAALSTFSLEDEQPEVQGPAVMVSAERAATDSPIEYSDVAAYRLSLSEDTKALNQLNTLIQEGKEMASILYTYRSCVKALPQLPESMKHSQADLYLETYQVLDLEMSRLREIQRWQSSASAKLAADMQRFSRPERRINGPTVTHLWSMLKLLDVLVQLDHLKNAKASIPNDFSWYKRTFTQVSAQWQDTDTMREELDDLQIFLSTRWAILLNLHVEMFRVNNVEDILQVLIVFIVESLELDFALLFPERYILLRVLPVLVVLATPSEKDTEALYKRVKLNRLINIFKNDPVIPAFPDLHLSPAAILKELSVYFQKFSSQTRLLTLPAPHELPPREALEYQRHYLIVNHIGALRAEHDDFTIRFASSMNQLLLLKSNDGAYTEWCREVKGNMYDMVVEGFQLLSRWTARIWEQCAWKFSRPCRDAGETPEASGSYSDYEKVVRFNYTAEERKALVELVGYIKSVGSMLQRCDTLVADALWETIHAEVQDFVQNTLATMLRTTFRKKKDLSRILSDMRTLSADWMANTRPEHEMPSSQHGNDESRGNFFYPRPVAPTAAQVHCLQFLIYEVVSGGNLRRPGGFFGNNGSEIPVNDLKQLETFFYKLSFFLHILDYSASIGILTDLGFLWFREFYLESSRVIQFPIECSLPWMLIDYILEAQNSGLLESVLLPFDIYNDSAQQALVVLRQRFLYDEIEAEVDHGFDIFVSRLSESIFTYYKSWSASELLDPSFLFALDNGEKFSIQPVRFTALFKMTKVKILGRTINLRSLIAQRMNRIFRENLEFLFDRFESQDLCAVVELEKLIDILKHSHELLSQDLSIDPFSLMLNEMQENISLVSFSSRLATQIWSEMQSDFLPNFILCNTTQRFVRSSKVPPTQKPSVPSAKPSFYCGTQDLNAAHQSFARLHSGFFGIPHLFSIVKLLGSRSLPWLIRALLDHISNKITTLEPMISGLQEALPKSIGLLSFDGGVTGCMKLIREQLNWGTKSELKSEVLRGIKEIGSVIYTMGLLDIVLREVDTKRFMQTAPWLGLIPGAEGQIVNAQDGESPLVNLLKSATSAVVSSPGCLNPAAFYTMSKQAEAADLLYKANMNGGSVLEYTLAFTSASLDKYCSKWSAPPKTGFVDITTSKDFYRIYGGLQIGYLEEITAPQSAQHEVLGDSIAWGGCTIIYLLGQQLHFELFDFSYQVLNVSEVETVSASHTHRNPQIHQGWEGLLEGMKKARRLNNHVFSMLKARCPLEDKTACAIKQSGAPLPRVRFENTVSAFETLPQKGTVG nckp1_arath Protein NAP1 Nck-associated protein 1 p125Nap1 NAP of plants ARP2/3 regulatory protein subunit NAPP Protein GNARLED NAP1 GRL NAP NAPP T4C15.22 At2g35110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSRQYYPSQDESMSPTSVRSREWEGPSRWTEYLGPEMAASVSSTRSSKQIDGHVGGSTKALNIQWVVQMIEVADGLMAKMYRLNQILEYPDPVGHVFSEAFWKAGVFPNHPRICTLLSKKFPEHFSKLQLERIDKFSLDSLHDGAELHLQSLEPWIQLLLDLMAFREQALRLILDLSSTVITLLPHQNSLILHAFMDLFCAFVRVNLFAEKIPRKMLLQVYNLLHALSRNDRDCDFYHRLVQFIDSYDPPLKGLQEDLNFVSPRIGEVLEAVGPSIFLSADTRKLRNEGFLSPYHPRFPDILTNSAHPMRAQDLANVTSYREWVLLGYLVCPDELLRVTSIDIALVVLKENLVVTLFRDEVSLYQMVCEKEFGIGISFASADSINLTMQYILLHEDYQLYVLPRVLESKKMAKSGRTKQKEADLEYSVAKQVEKMISEVHEQALQLCDTIHRERRILLKQEIGRMVLFFTDQPSLLAPNIQMVFSALALAQSEVLWYFQHAGIASSRSKAARVIPVDIDPNDPTIGFLLDGMDRLCCLVRKYISAARGYALSYLSSSAGRIRYLMGTPGIVALDLDPTLKGLFQRIVQHLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLSEGNAAYNWSRCVDELESQLSKHGSLKKLYFYHQHLTTVFRNTMFGPEGRPQHCCAWLSVASSFPECASLIIPEEVTKFGRDAVLYVESLIESIMGGLEGLINILDSEGGFGALESQLLPEQAAAYLNNASRISAPSVKSPRVVGGFTLPGHESYPENNKSIKMLEAAIQRLTNLCSILNDMEPICVINHVFVLREYMRECILGNFKRRFLTALQTDNDLQRPSVLESLIRRHMGIVHLAEQHVSMDLTQGIREILLTEAFSGPVSSLHTFEKPAEQQQTTGSAVEVVCNWYMDNIIKDVSGAGILFAPRHKYFKSTRPVGGYFAESVTDLKELQAFVRIFGGYGVDRLDRMMKVHTAALVNCIETSLRSNRELIEAAAASMHSGDRVERDASVRQIVDLDTVIGFCIEAGQALAFDDLLAEASGAVLEDNASLIHSMISGIVEHIPEEIPEKKEIRRIKGVANGVGVAGDHDSEWVRLILEEVGGANDNSWSLLPYFFASFMTSNAWNTTGFNIETGGFSNNIHCLARCISAVIAGSEYVRLQREYQQQHQSLSNGHHSSENLDSEFPPRVTAEASIKSSMLLFVKFAASIVLDSWSEANRSHLVAKLIFLDQLCEISPYLPRSSLESHVPYTILRSIYTQYYSNTPSTPLSTASPYHSPSVSLIHASPSMKNSTTPQRGSGSGSSSTAAPDSGYFKGSSSSLYGQEHYTESETGNSRNNENNNNNKQRGSSRRSGPLDYSSSHKGGSGSNSTGPSPLPRFAVSRSGPISYKQHN cyfp1_human Cytoplasmic FMR1-interacting protein 1 Specifically Rac1-associated protein 1 p140sra-1 CYFIP1 KIAA0068 protein protein human Homo sapiens Human MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSGSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDSAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTRFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSIVELDGLLEINRMTHKLLSRYLTLDGFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGEGTPVEHVRCFQPPIHQSLASS nckp1_human Nck-associated protein 1 NCKAP1 HEM2 KIAA0587 NAP1 p125Nap1 Membrane-associated protein HEM-2 protein protein human Homo sapiens Human MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA rac4_arath Rac-like GTP-binding protein ARAC4 ARAC4 ROP2 T20H2.12 At1g20090 GTPase protein ROP2 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASRFIKCVTVGDGAVGKTCMLISYTSNTFPTDYVPTVFDNFSANVVVDGNTVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENIAKKWIPELRHYAPGVPIILVGTKLDLRDDKQFFIDHPGAVPITTNQGEELKKLIGSAVYIECSSKTQQNVKAVFDAAIKVVLQPPKQKKKKKNKNRCAFL rac1_human Ras-related C3 botulinum toxin substrate 1 RAC1 MIG5 p21-Rac1 Ras-like protein TC25 Cell migration-inducing gene 5 protein TC25 protein protein human Homo sapiens Human MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL klk-nap125-2 Interaction between KLK (PIR) and NAP125 (NAP1) proteins 673562 N/A 673564 unspecified role unspecified role bait bait N/A 673565 unspecified role unspecified role prey prey physical association physical association false false false 4 sra1-nap125 Interaction between Human SRA1 and Arabidopsis NAP125 proteins 673562 N/A 673566 unspecified role unspecified role bait bait N/A 673565 unspecified role unspecified role prey prey physical association physical association false false false 4 sra1-nap Interaction between human SRA1 (CYFIP1) and human NAP protein (NCKAP1) 673562 N/A 673567 unspecified role unspecified role prey prey N/A 673566 unspecified role unspecified role bait bait physical association physical association false false false 4 klk-rop2 Interaction between KLK (PIR) and ROP2 (ARAC4) proteins 673562 N/A 673568 unspecified role unspecified role prey prey Q38919:p.Asp120Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false Q38919:p.Thr19Asn mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false N/A 673564 unspecified role unspecified role bait bait physical association physical association false false false 5 klk-rac1 Interaction between Arabidopsis KLK (PIR) and Human RAC1 proteins 673562 N/A 673569 unspecified role unspecified role prey prey N/A 673564 unspecified role unspecified role bait bait physical association physical association false false false 5 sra1-rop2 Interaction between Human SRA1 (CYFIP1) and Arabidopsis ROP2 (ARAC4) protein 673562 N/A 673566 unspecified role unspecified role bait bait N/A 673568 unspecified role unspecified role prey prey physical association physical association false false false 5 sra1-rac1 Interaction between Human SRA1 (CYFIP1) and Human RAC1 (NCKAP1) protein 673562 N/A 673567 unspecified role unspecified role prey prey N/A 673566 unspecified role unspecified role prey prey physical association physical association false false false 5 klk-nap125-1 Interaction between KLK (PIR) and NAP125 (NAP1) proteins 673563 N/A 673565 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 673564 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecobd Escherichia coli (strain B / BL21-DE3) physical association physical association false false false 4