IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ feng-2004b-1 Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng S., Shen Y., Sullivan JA., Rubio V., Xiong Y., Sun TP., Deng XW. Transgenic Arabidopsis plants expressing FLAG tagged CAND1 protein were used in this study. xingwang.deng@yale.edu Plant Cell (1040-4651) 2004 Only protein-protein interactions imex curation feng-2004b-2 Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng S., Shen Y., Sullivan JA., Rubio V., Xiong Y., Sun TP., Deng XW. Transgenic Arabidopsis plants expressing FLAG tagged CAND1 protein were used in this study. xingwang.deng@yale.edu Plant Cell (1040-4651) 2004 Only protein-protein interactions imex curation feng-2004b-3 Arabidopsis CAND1, an unmodified CUL1-interacting protein, is involved in multiple developmental pathways controlled by ubiquitin/proteasome-mediated protein Degradation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Feng S., Shen Y., Sullivan JA., Rubio V., Xiong Y., Sun TP., Deng XW. Transgenic Arabidopsis plants expressing FLAG tagged CAND1 protein were used in this study. xingwang.deng@yale.edu Plant Cell (1040-4651) 2004 Only protein-protein interactions imex curation cand1_arath Cullin-associated NEDD8-dissociated protein 1 At2g02560 CAND1 ETA2 HVE Cullin-associated and neddylation-dissociated protein 1 Protein ENHANCER OF TIR1-1 AUXIN RESISTANCE 2 Protein HEMIVENATA T8K22.14 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANLQVSGILEKFQMTGKDKDYRYMATSDLLNELNKDSFKIDLDLEVRLSSIILQQLDDVAGDVSGLAVKCLAPLVKKVGEERIVEMTNKLCDKLLHGKDQHRDTASIALRTVVAQIAPTLAPSILVTLTPQMIGGISGQGMSSGIKCECLEIMCDVVQKYGSLMTDDHEKLLNTLLLQLGCNQATVRKKTVTCIASLASSLSDDLLAKATVEVVKNLSNRNAKSEITRTNIQMIGALCRAVGYRFGTHLGNTVPVLINYCTSASENDEELREYSLQALESFLLRCPRDISPYCDEILNLTLEYISYDPNFTDNMEEDTDNETLEDEEDDESANEYTDDEDASWKVRRAAAKCLAGLIVSRSEMLTKVYQEACPKLIDRFKEREENVKMDVFNTFIDLLRQTGNVTKGQTDTDESSPKWLLKQEVSKIVKSINRQLREKSVKTKVGAFSVLRELVVVLPDCLADHIGSLVPGIERALNDKSSTSNLKIEALVFTKLVLASHAPPVFHPYIKALSSPVLAAVGERYYKVTAEALRVCGELVRVVRPSTAGMGFDFKPFVHPIYNAIMSRLTNQDQDQEVKECAITCMGLVISTFGDQLRAELPSCLPVLVDRMGNEITRLTAVKAFSVIATSPLHINLSCVLDHLIAELTGFLRKANRVLRQATLITMNTLVTAYGDKIGSEAYEVILVELSSLISVSDLHMTALALELCCTLMTGKSCSENISLAVRNKVLPQALTLVKSPLLQGQALLDLQKFFEALVYHANTSFYTLLESLLSCAKPSPQSGGVPKQALYSIAQCVAVLCLAAGDKNCSSTVKMLMEILKDDSGTNSAKQHLALLSLGEIGRRKDLSAHAGIETIVIESFQSPFEEIKSAASYALGNIAVGNLSNYLPFILDQIDNQQKKQYILLHSLKEVIVRQSVDKADFQNSSVEKILALLFNHCESEEEGVRNVVAECLGKMALIEPEKLVPALQVRTTSPAAFTRATVVTAVKYSVVERPEKLDEIIFPQISSFLMLIKDGDRHVRRAAVSALSTFAHYKPNLIKGLLPELLPLLYDQTVIKKELIRTVDLGPFKHVVDDGLELRKAAFECVFTLVDSCLDQVNPSSFIVPFLKSGLEDHYDLKMLCHLILSLLADKCPSAVLAVLDSLVEPLHKTISFKPKQDAVKQEHDRNEDMIRSALRAISSLDRINGVDYSHKFKGLMGDMKRSVPLWEKFQTIRNE B0F5769754DE6040 cul1_arath Cullin-1 CUL1 At4g02570 T10P11.26 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERKTIDLEQGWDYMQTGITKLKRILEGLNEPAFDSEQYMMLYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHDEFMLRELFKRWSNHKVMVRWLSRFFYYLDRYFIARRSLPPLNEVGLTCFRDLVYNELHSKVKQAVIALVDKEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRDDKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQVLIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILKKGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILTKLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSYKSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQAAVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEFNSKFTDRMRRIKIPLPPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLVSECVEQLSRMFKPDIKAIKKRMEDLITRDYLERDKENPNMFRYLA cand1-cul1-6 CAND1 interacts with Cul1 678121 N/A 678124 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 678125 unspecified role unspecified role prey prey physical association physical association false false false 3a cul1-cand1-9 Cul1 interacts with CAND1 678122 N/A 678125 unspecified role unspecified role bait bait c-terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 678124 unspecified role unspecified role prey prey physical association physical association false false false 3b cand1-cul1-7 CAND1 interacts with Cul1 678123 N/A 678124 unspecified role unspecified role bait bait N/A 678125 unspecified role unspecified role prey prey Q94AH6:p.Lys682Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3d CAND1 interacts with Cul1 (K682R) more strongly than with wild-type CUL1 in yeast two-hybrid assays.