UniProt UniProt http://www.uniprot.org malone-2003-1 The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Malone CJ., Misner L., Le Bot N., Tsai MC., Campbell JM., Ahringer J., White JG. cjmalone@facstaff.wisc.edu Cell (0092-8674) 2003 Only protein-protein interactions imex curation malone-2003-2 The C. elegans hook protein, ZYG-12, mediates the essential attachment between the centrosome and nucleus. caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Malone CJ., Misner L., Le Bot N., Tsai MC., Campbell JM., Ahringer J., White JG. cjmalone@facstaff.wisc.edu Cell (0092-8674) 2003 Only protein-protein interactions imex curation q23529-2 Zygote defective protein 12 zyg-12 ber-1 ZK546.1 protein protein caeel Caenorhabditis elegans MLDLTNKESESSDNGNSKYEDSIDGREVGTSKPFKEERSLEDLQADLADMAVWMEGLDATKLPLNDPQLLCNGRAFSEVLHNVDKNFFTDGWLETMPENRTTNIMVFRSCTRKLWRKMFDYVNHINRTVVSSRWTDIHERIDGIYESDLPAMVNLGMAVVTLAHIGKNAKRFVDYSKALTSTHKSMMSNVAKMVTTVIDEMPENPCFHEISELHGSQSELNSLSESSGKLNGNGSSERRSNADQILVDAELEIERLRTETENQRKEIERLTKSFETAQHDMSSNSESGDISILEKQNEELRQKRRELEEKNLELDAAVDQFKGIVFELTNENDVLRRSDKERQRLQTVLDAAQSDLDEWKTVANQYQKEAELSKQQDKEIKELLSQNKALKSRLDHHVKSATLEDANKNGIAQLRTQVGGLTALNTELKASLDSKKRCVEQLEIQLIQHKEKVKELEDRKDELIEERNRLENQLIFKEAVTPRSLHESMFEAGNLSFEPFSEKNTLPLEIENKRLTERIQELESLEPLKGELITLKSKNGVLEEEKLFATKQIEELQQQIEDLQENLLKNQEHASGDVVGLKIQLEKAEVEAQQMREAKMRAETNQAQVDEILKKRTAELEVNATALQKAKAVIDELEYNSRPVSEDSMTSVQAFKEMKEENEKLRQKVEKLEIELNTVTQGFEQENRLLTSASHQQVLNRSIDEVMSMRAHAGSEEPQTLLDTQKMSGALPWRFS No experimental confirmation available. Does not contain a transmembrane domain. g5eck7_caeel Dynein light intermediate chain dli-1 C39E9.14 CELE_C39E9.14 protein protein caeel Caenorhabditis elegans MPPTAQPLEFDDTANAVLPVLYSSTDEEVGIWRELLASKKTNTNQGSTVIVLGDNRSGKSSLLSRLDKSDRTVKPKSLLGYRVLHVQNDARDASYAYQLGTAGANLNPSELLQIPVWSLDGNAACASLLKHALPATPAEAIFILTASIDNPNLIHSLKRWANVCTEQAQKHFTKEDLKAGRSQQEKMWQEYVDPVQSQMSTSVVGSFADEHNLLPLDQGTLTENCGVTFMVVITKSDLGKEFTDAQFAKISIQLRKFCLSLGATLVFTSAKETKNIQLLQKYIVHRSFGTAFTSAAQVIERESIFVPAGWDGEKKIDIIRESIPDVDSALEPTRDKLRPVAKEQLIEAEEDQAFLKKLMDILATSTTTAAPKPRTMQEEPTDKDSPLANFFSNLLNKDKPSKVSSPSTVSQPMDAAATQAQLDRLLKSAQSPKPQPRDSDA hook_caeel Zygote defective protein 12 zyg-12 ber-1 ZK546.1 protein protein caeel Caenorhabditis elegans MLDLTNKESESSDNGNSKYEDSIDGREVGTSKPFKEERSLEDLQADLADMAVWMEGLDATKLPLNDPQLLCNGRAFSEVLHNVDKNFFTDGWLETMPENRTTNIMVFRSCTRKLWRKMFDYVNHINRTVVSSRWTDIHERIDGIYESDLPAMVNLGMAVVTLAHIGKNAKRFVDYSKALTSTHKSMMSNVAKMVTTVIDEMPENPCFHEISELHGSQSELNSLSESSGKLNGNGSSERRSNADQILVDAELEIERLRTETENQRKEIERLTKSFETAQHDMSSNSESGDISILEKQNEELRQKRRELEEKNLELDAAVDQFKGIVFELTNENDVLRRSDKERQRLQTVLDAAQSDLDEWKTVANQYQKEAELSKQQDKEIKELLSQNKALKSRLDHHVKSATLEDANKNGIAQLRTQVGGLTALNTELKASLDSKKRCVEQLEIQLIQHKEKVKELEDRKDELIEERNRLENQLIFKEAVTPRSLHESMFEAGNLSFEPFSEKNTLPLEIENKRLTERIQELESLEPLKGELITLKSKNGVLEEEKLFATKQIEELQQQIEDLQENLLKNQEHASGDVVGLKIQLEKAEVEAQQMREAKMRAETNQAQVDEILKKRTAELEVNATALQKAKAVIDELEYNSRPVSEDSMTSVQAFKEMKEENEKLRQKVEKLEIELNTVTQGFEQENRLLTSASHQQVLNRSIDEVMSMRAHAGSEEPQTLLDTQKMSGALPWRSLASETRRELPTAMASILVLGFLVFIAWMFININSALNAPPNA zyg12-zyg12-1 684489 N/A 684491 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q23529-2:p.Gln44Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q23529-2:p.Gln367Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false N/A 684491 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q23529-2:p.Gln44Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q23529-2:p.Gln367Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 6A zyg12-dli1-1 684489 N/A 684491 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q23529-2:p.Gln44Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false N/A 684492 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 6A zyg12-zyg12-2 684490 N/A 684493 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 684493 unspecified role unspecified role prey prey physical association physical association false false false Fig 6B