IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ nekrasov-2003-1 Interactions between centromere complexes in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Nekrasov VS., Smith MA., Peak-Chew S., Kilmartin JV. jvk@mrc-lmb.cam.ac.uk Mol. Biol. Cell (1059-1524) 2003 nekrasov-2003-2 Interactions between centromere complexes in Saccharomyces cerevisiae. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nekrasov VS., Smith MA., Peak-Chew S., Kilmartin JV. jvk@mrc-lmb.cam.ac.uk Mol. Biol. Cell (1059-1524) 2003 nsl1_yeast Kinetochore-associated protein NSL1 NSL1 YPL233W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQGQSKKLDVTVEQLRSIYHQFHDILEEKTDLHLPKKEYDDDAVRREVQIQLQEFLLSAMTMASKSLEVVNADTVGKTVKQLIMESQEKYMEPFDLDLNEQVRKMYQEWEDETVKVAQLRQTGPAKINEVYNNSKDEYLAQLDGRIGVLQARMMQQQSADHDDSTDDADDHINWEHIKQDYVASLNELYQTQQDLPKVRYNVEKVKRLMDFLEED nnf1_yeast Kinetochore-associated protein NNF1 J2011 YJR112W NNF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVNSHGIRYIRLKQVFNRALDQSISKLQSWDKVSSCFPQYVNSKQGAINVANCQRQLTEFWTELCQREFKEIMEERNVEQKLNELDELILEAKERYTDRDQDEVNKGPAIDELSSKELVECHLYSQRMHAIHEIDERLAKVNEMNDQLAQELKDLETQVEVEKNEIGKMYDEYLGSHTDQPANVLLVQSLNDMVLELKENY dsn1_yeast Kinetochore-associated protein DSN1 YIB10W YIR010W DSN1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLEPTQTVSGTPPMLHQRTHKQVYPLRMETIPILESDSKATLQSNEPTQKDEEETEYFENKQSVSNLSPDLKFKRHKNKHIQGFPTLGERLDNLQDIKKAKRVENFNSSAPIADDNHSGDATANATANATANATANVNASAMPAPYMPYYYYYHPMNAPTPAMIPYPGSPMHSIMPNSSLQPFYSQPTAAGGPDMTTPQNISSSQQLLPAPQLFPYGSFHQQQLQQPHYIQRTRERKKSIGSQRGRRLSMLASQANGGSTIISPHKDIPEEDFYTVVGNASFGKNLQIRQLFNWCLMRSLHKLELKAKNQEEEGELEHLTKKSKLESTKAETDYVDPKRLAMVIIKEFVDDLKKDHIAIDWEDEEKYEDEDEEKILDNTENYDDTELRQLFQENDDDDDDDDEVDYSEIQRSRRKFSERRKALPKEPKKLLPNSKNVENTKNLSILTSKVNAIKNEVKEWAVTLDTSRPDLEWQELTSFSSQPLEPLSDTEEPDLAIADVETKLETKVDELRYQSHILNSHSLALNEITNSKVNKLNIETMRKISSETDDDHSQVINPQQLLKGLSLSFSKKLDL mtw1_yeast Kinetochore-associated protein MTW1 MTW1 YAL034W-A YAL034AW DSN3 NSL2 Mis12-like protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTMRSTSILTEHLGYPPISLVDDIINAVNEIMYKCTAAMEKYLLSKSKIGEEDYGEEIKSGVAKLESLLENSVDKNFDKLELYVLRNVLRIPEEYLDANVFRLENQKDLVIVDENELKKSEEKLREKVNDVELAFKKNEMLLKRVTKVKRLLFTIRGFKQKLNELLKCKDDVQLQKILESLKPIDDTMTLLTDSLRKLYVDSESTSSTEEVEALLQRLKTNGKQNNKDFRTRYIDIRTNNVLRKLGLLGDKEDEKQSAKPDARTQAGDIVSIDIEEPQLDLLDDVL kre28_yeast Spindle pole body component KRE28 KRE28 YDR532C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDTGSASIKDYETVLTDIEDSIAVSSEEVLNNQELRLKNTLHEITSSILAINEENKFVNPLRNDESLDVEGKEVFVNPKILSAKIKEFNKLMELLKLTYLEQETLDYFFRFTLSSTKPLQLDSEKDPQFVKLNERVNDLKEEISNVQESKIEQIKAEIQETGHNFAERQDLINELYLEATGDIENCWDSLNELKNLTNKEDKNMMGEKDTILNSSDSDDFVEETYTNWQKLLFLQKQNQRLTKELKEMHEVKNQIIRKGEQSKKEDSGHLMANESELCQSINLLTKFWEKHFLLKGSKTTILNFEIFTQLGKVQFEIKDMQYIIAISLSDLKRPMIKDITILQKAGGNIVTDIEASSKFNNKYRNNTKVQIFEVMDDIISELTNE A1A44CF0D9E75F50 sp105_yeast Spindle pole body component SPC105 SPC105 105 kDa spindle pole component protein YGL093W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNVDERSRIGGREKDAGPGKGILKQNQSSQMTSSFLENPGVRIPTRIITKKEVLDGSNTTSRINTSNLQSMVKRRVSFAPDVTLHSFTFVPEQNNEIKEPRRRKTSTNSPTKISSQEEPLVTSTQIDDARTEEKTAAEEDPDTSGMELTEPIVATPDSNKASQHDPTSMEMTEVFPRSIRQKNPDVEGESIESSQQIDDVEAVREETMELTAIHNVHDYDSISKDTVEGEPIDLTEYESKPYVPNSVSRSTGKSSDYSVERSNDKSDLSKSENKTNSSQPMEITDIFHADPQNPMSLHSDNNINNDGNEMELTQIQTNFDRDNHHIDESPSEKHAFSSNKRRKLDTVSDYAASVTTPVKEAKDTSGEDNDGDLEMMEKMSPITFSDVDNKIGTRSNDVFTIEPGTEDTGMQTATDDEEDGENVDDNGNKIVEKTRLPEIDKEGQSGIALPTQDYTLREFINEVGVGFLDTKLIDDLDKKVNFPLNSFNFVENQRIDNVFSAFYIDIPILEVEAFRCKELWRSINESKDKFKDFEAQIDKSHPPLLLQEYFSSDEKMKQLMRDQLQLVKGYSKLEAAMEWYEWRKKQLNGLELILAENLNTLKREYEKLNEEVEKVNSIRGKIRKLNEAIKEEIRSLKNLPSDSYKPTLMNRIKIEAFKQELMEHSISLSSSNDFTQEMRSLKLAIAKKSNDILTLRSEVASIDKKIEKRKLFTRFDLPKLRDTLKILESLTGVRFLKFSKATLSIAFLQLDDLRVDINLANFKNNPLSSMKVMNDSNNDDMSYHLFTMLLKNVEAEHQDSMLSNLFFAMKKWRPLLKYIKLLKLLFPVKITQTEEEEALLQFKDYDRRNKTAFFYVISLVSFAQGVFSENGQIPMKVHISTQQDYSPSREVLSDRITHKISGVLPSFTKSRIHLEFT spc24_yeast Kinetochore protein SPC24 SPC24 YM9718.16C YMR117C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEESEARISKEHSKVSESNKKLNAERINVAKLEGDLEYTNEESNEFGSKDELVKLLKDLDGLERNIVSLRSELDEKMKLYLKDSEIISTPNGSKIKAKVIEPELEEQSAVTPEANENILKLKLYRSLGVILDLENDQVLINRKNDGNIDILPLDNNLSDFYKTKYIWERLGK 8BE4B8619DD4C8FB spc25_yeast Kinetochore protein SPC25 YER018C SPC25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMRQLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTLAAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVRFVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLLASL 1BED6CCFF330A5D8 nuf2_yeast Kinetochore protein NUF2 YOL069W NUF2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRNQDVFPILDLQELVICLQSCDFALATQENISRPTSDYMVTLYKQIIENFMGISVESLLNSSNQETGDGHLQEENENIYLDTLNVLVLNKICFKFFENIGVQDFNMTDLYKPEAQRTQRLLSAVVNYARFREERMFDCNSFILQMESLLGQLRSKFDDYNLIQQQLKQYEDVDGDNIPDEQELQKLEEQNKELEIQLKKLTKIQETLSIDYNDYKISKQSIFKDLEALSFQIVELESNRDKLIKISNTDMEELSEGIKELNDLLIQRKKTLDDLTAQQKNLQDTVTTFETIISELYDVLRIISSEVQESNRTETELVGLKQNLINNKLKLMNVLETGIMYKLEILQEQLDLQLKNLEKLSQDTKEESRLNDTKLMDLQIKYENEIKPKIDKTDIFIQEELISGKINKLNDEIKQLQKDFEVEVKEIEIEYSLLSGHINKYMNEMLEYMQ ndc80_yeast Kinetochore protein NDC80 NDC80 80 kDa spindle component protein HEC1 Nuclear division cycle protein 80 TID3 Two-hybrid interaction with DMC1 protein 3 YIL144W protein protein yeast Saccharomyces cerevisiae Baker's yeast MQSSTSTDQHVLHHMDPHRFTSQIPTATSSQLRRRNSTNQGLTDMINKSIARNTISGTGIPTGGINKNKRTRSTVAGGTNGTALALNDKSNSRNSVSRLSINQLGSLQQHLSNRDPRPLRDKNFQSAIQEEIYDYLKKNKFDIETNHPISIKFLKQPTQKGFIIIFKWLYLRLDPGYGFTKSIENEIYQILKNLRYPFLESINKSQISAVGGSNWHKFLGMLHWMVRTNIKLDMCLNKVDRSLINQNTQEITILSQPLKTLDEQDQRQERYELMVEKLLIDYFTESYKSFLKLEDNYEPSMQELKLGFEKFVHIINTDIANLQTQNDNLYEKYQEVMKISQKIKTTREKWKALKSDSNKYENYVNAMKQKSQEWPGKLEKMKSECELKEEEIKALQSNISELHKILRKKGISTEQFELQNQEREKLTRELDKINIQSDKLTSSIKSRKLEAEGIFKSLLDTLRQYDSSIQNLTRSRSQLGHNVNDSSLKINISENLLDRDFHEGISYEQLFPKGSGINESIKKSILKLNDEIQERIKTIEKDNITLEKDIKNLKHDINEKTQINEKLELELSEANSKFELSKQENERLLVAQRIEIEKMEKKINDSNLLMKTKISDAEELVTSTELKLEELKVDLNRKRYKLHQQVIHVIDITSKFKINIQSSLENSENELGNVIEELRNLEFETEHNVTN 7DBC492227A80093 mtw1-dsn1 Nnf1p, Nsl1p, Dsn1p copurify with Mtw1p in yeast cell extracts 671799 N/A 671801 unspecified role unspecified role prey prey N/A 671802 unspecified role unspecified role prey prey N/A 671803 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1 A spc105-ydr532 Ydr532p copurifies with Spc105p in yeast cell extracts 671799 N/A 671805 unspecified role unspecified role prey prey N/A 671806 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 B ydr532-spc105 Spc105p copurifies with Ydr532p in yeast cell extracts 671799 N/A 671805 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671806 unspecified role unspecified role prey prey physical association physical association false false false 1 B nnf1-dsn1-1 Mtw1p, Nsl1p, Dsn1p copurify with Nnf1p in yeast cell extracts 671799 N/A 671802 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671801 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role prey prey N/A 671803 unspecified role unspecified role prey prey association association false false false 1 D dsn1-mtw1-1 Nnf1p, Nsl1p, Mtw1p as well as components from Spc105p and Ndc80p complexes copurify with Dsn1p in yeast cell extracts 671799 N/A 671807 unspecified role unspecified role prey prey N/A 671808 unspecified role unspecified role prey prey N/A 671806 unspecified role unspecified role prey prey N/A 671809 unspecified role unspecified role prey prey N/A 671805 unspecified role unspecified role prey prey N/A 671810 unspecified role unspecified role prey prey N/A 671801 unspecified role unspecified role prey prey N/A 671803 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671802 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role prey prey association association false false false 1 E nsl1-dsn1 Mtw1p, Nsl1p, Dsn1p and Spc105p copurify with Nsl1p in yeast cell extracts 671799 N/A 671806 unspecified role unspecified role prey prey N/A 671802 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role prey prey N/A 671801 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671803 unspecified role unspecified role prey prey association association false false false 1 F mtw1-dsn1-1 Nnf1p, Nsl1p, Dsn1p and components of the Ndc80p and Spc105p complexes copurify with Mtw1p in yeast cell extracts 671799 N/A 671805 unspecified role unspecified role prey prey N/A 671808 unspecified role unspecified role prey prey N/A 671807 unspecified role unspecified role prey prey N/A 671809 unspecified role unspecified role prey prey N/A 671806 unspecified role unspecified role prey prey N/A 671810 unspecified role unspecified role prey prey N/A 671802 unspecified role unspecified role prey prey N/A 671801 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671803 unspecified role unspecified role prey prey association association false false false 1 G tid3-mtw1 Ndc80p complex components and Mtw1p copurify with Ndc80p 671799 N/A 671807 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role prey prey N/A 671808 unspecified role unspecified role prey prey N/A 671809 unspecified role unspecified role prey prey N/A 671810 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1 H spc105-mtw1 Ndc80p, Nuf2, Ydr532, Mtw1p and Nsl1p copurify with Spc105p 671799 N/A 671805 unspecified role unspecified role prey prey N/A 671810 unspecified role unspecified role prey prey N/A 671804 unspecified role unspecified role prey prey N/A 671809 unspecified role unspecified role prey prey N/A 671801 unspecified role unspecified role prey prey N/A 671806 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1 I tid3-spc105p Ndc80p-protein A coimmunoprecipitates Spc105p in yeast cell extracts 671800 N/A 671810 unspecified role unspecified role bait bait region protein a tag protein a tag protein A protein A undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671806 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 1 J