IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ benjamins-2003-1 PINOID-Mediated Signaling Involves Calcium-Binding Proteins yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rene Benjamins, Carlos S. Galva�n Ampudia, Paul J.J. Hooykaas, and Remko Offringa offringa@rulbim.leidenuniv.nl Arabidopsis cDNA library was constructed using mRNA isolated from green parts of 6-week-old flowering Arabidopsis plants. Plant Physiology 2003 benjamins-2003-2 PINOID-mediated signaling involves calcium-binding proteins. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Rene Benjamins, Carlos S. Galva�n Ampudia, Paul J.J. Hooykaas, and Remko Offringa offringa@rulbim.leidenuniv.nl Arabidopsis cDNA library was constructed using mRNA isolated from green parts of 6-week-old flowering Arabidopsis plants. Plant Physiology 2003 benjamins-2003-3 PINOID-mediated signaling involves calcium-binding proteins. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rene Benjamins, Carlos S. Galva�n Ampudia, Paul J.J. Hooykaas, and Remko Offringa offringa@rulbim.leidenuniv.nl Arabidopsis cDNA library was constructed using mRNA isolated from green parts of 6-week-old flowering Arabidopsis plants. Plant Physiology 2003 benjamins-2003-4 PINOID-mediated signaling involves calcium-binding proteins. in vitro In vitro in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Rene Benjamins, Carlos S. Galva�n Ampudia, Paul J.J. Hooykaas, and Remko Offringa offringa@rulbim.leidenuniv.nl Arabidopsis cDNA library was constructed using mRNA isolated from green parts of 6-week-old flowering Arabidopsis plants. Plant Physiology 2003 benjamins-2003-5 PINOID-Mediated Signaling Involves Calcium-Binding Proteins yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rene Benjamins, Carlos S. Galva�n Ampudia, Paul J.J. Hooykaas, and Remko Offringa offringa@rulbim.leidenuniv.nl Arabidopsis cDNA library was constructed using mRNA isolated from green parts of 6-week-old flowering Arabidopsis plants. Plant Physiology 2003 pid_arath Protein kinase PINOID Protein kinase ABRUPTUS T31E10.1 PID ABR At2g34650 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLRESDGEMSLGTTNSPISSGTESCSSFSRLSFDAPPSTIPEEESFLSLKPHRSSDFAYAEIRRRKKQGLTFRDFRLMRRIGAGDIGTVYLCRLAGDEEESRSSYFAMKVVDKEALALKKKMHRAEMEKTILKMLDHPFLPTLYAEFEASHFSCIVMEYCSGGDLHSLRHRQPHRRFSLSSARFYAAEVLVALEYLHMLGIIYRDLKPENILVRSDGHIMLSDFDLSLCSDSIAAVESSSSSPENQQLRSPRRFTRLARLFQRVLRSKKVQTLEPTRLFVAEPVTARSGSFVGTHEYVAPEVASGGSHGNAVDWWAFGVFLYEMIYGKTPFVAPTNDVILRNIVKRQLSFPTDSPATMFELHARNLISGLLNKDPTKRLGSRRGAAEVKVHPFFKGLNFALIRTLTPPEIPSSVVKKPMKSATFSGRSSNKPAAFDYF pbp1_arath Calcium-binding protein PBP1 PINOID-binding protein 1 F24B18.11 KRP2 KIC-related protein 2 PBP1 At5g54490 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASPKSSTRPNQENQEPQFQDFFPTMAGKLGGEGLIEEICKGFELLMDKDKGVITFESLRRNASTVLGLGDLTDDDVRYMINEGDFDRDGALNQMEFCVLMFRLSPELMEASRCVVTEVIEEEFYRH cml12_arath Calmodulin-like protein 12 At2g41100 CML12 CAL4 TCH3 Touch-induced calmodulin-related protein 3 T3K9.13 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MADKLTDDQITEYRESFRLFDKNGDGSITKKELGTMMRSIGEKPTKADLQDLMNEADLDGDGTIDFPEFLCVMAKNQGHDQAPRHTKKTMADKLTDDQITEYRESFRLFDKNGDGSITKKELRTVMFSLGKNRTKADLQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAPRHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMRSLGETQTKAELQDMINEADADGDGTISFSEFVCVMTGKMIDTQSKKETYRVVNQGQGQVQRHTRNDRAGGTNWERDIAVGVASNIIASPISDFMKDRFKDLFEALLS mbp_bovin Myelin basic protein MBP Myelin A1 protein 20 kDa microtubule-stabilizing protein protein protein bovin Bos taurus (Bovine) Bovine AAQKRPSQRSKYLASASTMDHARHGFLPRHRDTGILDSLGRFFGSDRGAPKRGSGKDGHHAARTTHYGSLPQKAQGHRPQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQKPGFGYGGRASDYKSAHKGLKGHDAQGTLSKIFKLGGRDSRSGSPMARR pid-pbp1 Yeast 2 hybrid analysis reveals interaction between PID protein and the PID binding protein 665400 N/A 665405 unspecified role unspecified role bait bait N/A 665406 unspecified role unspecified role prey prey physical association physical association false false false Table 1 pid-tch3 Yeast 2 hybrid analysis reveals interaction between PID protein and the TCH3 protein 665400 n/a 665407 unspecified role unspecified role prey prey N/A 665405 unspecified role unspecified role bait bait physical association physical association false false false Table 1 pid-tch3-2 Pull down analysis reveals interaction between PID protein and the TCH3 protein 665401 n/a 665407 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecobb Escherichia coli (strain B / BL21) N/A 665405 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false Figure 3 and 4 The interaction was dependent on the presence of calcium in the binding buffer. pid-pbp1-2 Pull down analysis reveals interaction between PID protein and the PID binding protein 665401 N/A 665405 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 665406 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecobb Escherichia coli (strain B / BL21) physical association physical association false false false The interaction was enhanced in the presence of calcium in the binding buffer. Figure 3 and 4 pid-pbp1-1 Yeast 2 hybrid analysis reveals interaction between PID protein and the PID binding protein 665402 N/A 665405 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 665406 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 pid-tch3-1 Yeast 2 hybrid analysis reveals interaction between PID protein and the TCH3 protein 665402 N/A 665405 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false n/a 665407 unspecified role unspecified role prey prey physical association physical association false false false Figure 2 pid-mbp Kinase assay for Arabidopsis PID protein and Bovine myelin basic protein 665403 N/A 665405 enzyme enzyme neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 665408 enzyme target enzyme target substrate substrate neutral component neutral component phosphorylation phosphorylation reaction phosphorylation false false false 6 phosphorylation of myelin basic protein. pid-pid Kinase assay for Arabidopsis PID protein 665403 N/A 665405 enzyme target enzyme target substrate substrate unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 665405 enzyme enzyme unspecified role unspecified role region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false 6 The authors comment that "Interestingly, autophosphorylation of GST:PID was reproducibly enhanced in the presence of PBP1." autophosphorylation of PID protein. pid-pbp1-3 Yeast 2 hybrid analysis reveals interaction between PID protein and the PID binding protein 665404 N/A 665405 unspecified role unspecified role bait bait N/A 665406 unspecified role unspecified role prey prey physical association physical association false false false Table 1 pid-tch3-3 Yeast 2 hybrid analysis reveals interaction between PID protein and the TCH3 protein 665404 N/A 665405 unspecified role unspecified role bait bait n/a 665407 unspecified role unspecified role prey prey physical association physical association false false false Table 1