IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ myers-2002-1 The yeast capping enzyme represses RNA polymerase II transcription. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography fingerprinting peptide massfingerprinting fingerprinting Myers LC., Lacomis L., Erdjument-Bromage H., Tempst P. Complex isolated by anion exchange, hydroxylapatite (HAP), and hydrophobic-chromatography, followed by final resolution on a Mono-Q column. larry.myeers@dartmouth.edu Mol. Cell (1097-2765) 2002 Only protein-protein interactions imex curation myers-2002-2 The yeast capping enzyme represses RNA polymerase II transcription. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Myers LC., Lacomis L., Erdjument-Bromage H., Tempst P. Participants identified by immunodepletion in that a DE400 fraction was incubated with anti-Cet1p antibodies immobilized on protein-A beads, and the supernatant was recovered. An equal volume of load (DE400) and supernatant (DE400[minusCet1/Ceg1]) was analyzed by Western blotting. larry.myeers@dartmouth.edu Mol. Cell (1097-2765) 2002 Only protein-protein interactions imex curation Complex isolated by anion exchange, hydroxylapatite (HAP), and hydrophobic-chromatography, followed by final resolution on a Mono-Q column. mce1_yeast mRNA-capping enzyme subunit alpha CEG1 YGL130W G2853 mRNA guanylyltransferase GTP--RNA guanylyltransferase protein protein yeast Saccharomyces cerevisiae Baker's yeast MVLAMESRVAPEIPGLIQPGNVTQDLKMMVCKLLNSPKPTKTFPGSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYLVNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRCLTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLDKLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDDRNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSDEEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSVSLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPKYVDEDDWSD F7F5A3E59EEFA15F cet1_yeast mRNA-capping enzyme subunit beta CET1 mRNA 5'-phosphatase mRNA 5'-triphosphate monophosphatase P1433 YPL228W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYTDNPPQTKRALSLDDLVNHDENEKVKLQKLSEAANGSRPFAENLESDINQTETGQAAPIDNYKESTGHGSHSQKPKSRKSSNDDEETDTDDEMGASGEINFDSEMDFDYDKQHRNLLSNGSPPMNDGSDANAKLEKPSDDSIHQNSKSDEEQRIPKQGNEGNIASNYITQVPLQKQKQTEKKIAGNAVGSVVKKEEEANAAVDNIFEEKATLQSKKNNIKRDLEVLNEISASSKPSKYRNVPIWAQKWKPTIKALQSINVKDLKIDPSFLNIIPDDDLTKSVQDWVYATIYSIAPELRSFIELEMKFGVIIDAKGPDRVNPPVSSQCVFTELDAHLTPNIDASLFKELSKYIRGISEVTENTGKFSIIESQTRDSVYRVGLSTQRPRFLRMSTDIKTGRVGQFIEKRHVAQLLLYSPKDSYDVKISLNLELPVPDNDPPEKYKSQSPISERTKDRVSYIHNDSCTRIDITKVENHNQNSKSRQSETTHEVELEINTPALLNAFDNITNDSKEYASLIRTFLNNGTIIRRKLSSLSYEIFEGSKKVM 2B946CC53D66F824 ceg1-cets-1 Identification of Cet1 and Ceg1 as components of the mRNA capping enzyme identified by mass spectrometery 606741 N/A 606743 unspecified role unspecified role neutral component neutral component N/A 606744 unspecified role unspecified role neutral component neutral component physical association physical association false false false 3 ceg1-cets-2 Interaction between Cet1 and Ceg1 identified by immunodepletion 606742 N/A 606744 unspecified role unspecified role bait bait N/A 606743 unspecified role unspecified role prey prey physical association physical association false false false 5