UniProt UniProt http://www.uniprot.org wang-2002d-1 The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Wang CW., Stromhaug PE., Shima J., Klionsky DJ. klionsky@umich.edu J. Biol. Chem. (0021-9258) 2002 wang-2002d-2 The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation anti tag western anti tag western blot anti tag western Wang CW., Stromhaug PE., Shima J., Klionsky DJ. Linear density gradient consisting of 10-55 percent OptiPrep used. klionsky@umich.edu J. Biol. Chem. (0021-9258) 2002 wang-2002d-3 The Ccz1-Mon1 protein complex is required for the late step of multiple vacuole delivery pathways. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang CW., Stromhaug PE., Shima J., Klionsky DJ. klionsky@umich.edu J. Biol. Chem. (0021-9258) 2002 ccz1_yeast Vacuolar fusion protein CCZ1 YBR131W YBR1006 CVT16 CCZ1 AUT11 Calcium-caffeine-zinc sensitivity protein 1 Autophagy-related protein 11 Cytoplasm to vacuole targeting protein 16 protein protein yeast Saccharomyces cerevisiae Baker's yeast MRLHYITVFDPSRSTNENDTFKQLLLFHYFGTTDSIPSLNEKLSIIGVIQGIWSLTSSCVNKDGEDLEKIIELNNDIIFCIKVESRFFISLAISNISDDQSAIPLQYLSAYLWLSYRFFKLLNGSFSGFNKDFRKLTDLLNEFVIPFWNDIYLNLETVTNRSFTVMWPGFYKRANFQHSSYNPGEKNNVEESWDAIILQNILLDKKSYLGLKDILVYHLPKRTKAANRESMGTKTYGLVRNFTSDLNTLPDISNWLYHLHCTYGEISSHILTGNVHFKEELQVEEEQERSRDTNGRDEEESQEQQRREHQETTQNNTSELSLSERVIHNVTLPISFAYDAIHEVSTTTGVSGSLSMIMDYVPKPHWPFISSSNKSADKNNYSNSNDNANSNAPLMAQSEAVGGTIGNSRFGFLISPLNSDLLPSSYQALKLNLNFENSKDKEDFYNCLFWYFDDFLIVIVCDPDFNKICERDYLKDLSFQLCQSMECLNNEILNSQNCDNVESFAYVIRDNVTKEIDSSVPFGSPKFTSDESISTLQLAINGIDQFINDNSNSLSLANWNPITIMGGSNAISKKNTTEGFGNGVNDKTQKFKRKYLNFLNLMSAEKLWDLQVDVLQFLTSLQNSKRDPDYFQEERLLKLNNGVLCYIKENNSNLIIIIKNWFQNNGTSKAAKQRNRFSSDSSKGSSLFQSLGRDVTDWWESREI mon1_yeast Vacuolar fusion protein MON1 YGL124C G2889 MON1 AUT12 Monensin sensitivity protein 1 Autophagy-related protein 12 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNLNESYLDAEIPKGQLKHSKSGNFEGIPIVATTSEPTTSVNLDETFFKKAPIAMPICDDHSVSKSTSVNSLNTTSLASRRSPLQTKKLQAKNNLLSADLAKSNDDTTRALNSPKKDFGPYLDSENDIRSRLAESIYSMETSIRGSELQRRPYVSNEIPNVFKFSKFNSNCKLNESQTLCDKNFFIFTSAGKPIYCMHGKDEQIMSYTGLVNTVISYFQVNGPSELKTISTLTSGKRLTFLDKSPILLMAQSERGESSNELLNQLDFLYSYILSSLSERQLLRLFSKRENFDLRNYLESTDFENLDEICSLICNRMFPDLLLNSLQCLPFNHSSRLKLQNVVLQQLEKRQDIPRGTLLYGLIIAPQNKLCCVLRPRGHTLHTTDLHLLFCLISHQFQNLDETQELWVPICFPKFNSSGFLYCYIKFLPNDTHSNEKSALVLISAQKDAFFSLKSFSDELIIKLEEEKLLKKINTSKGFKLSDIPAPMVHHFIYKSKQNVQYVMPHFEVNSNIALDSSQGLEYELKLKTYYQQLHGTVVRDNGNLLSRSMLNFVRWSSKDNEDLAMDETQMDFSELDEYIIGNSSFKQESVNMVGMAWVTPTFELYLIGNNGIVDKRVLFKSARKVANWCQKHESRLFISDGAVF ccz1-mon1 Ccz1 and Mon1 co-localize to a perivacuolar compartment different from the pre-autophagosomal structure 592816 N/A 592819 unspecified role unspecified role neutral component neutral component c-terminus cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 592820 unspecified role unspecified role neutral component neutral component n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false Fig. 6A ccz1-mon1-1 592817 N/A 592820 unspecified role unspecified role neutral component neutral component c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 592819 unspecified role unspecified role neutral component neutral component c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 7A ccz1-mon1-2 592818 N/A 592820 unspecified role unspecified role prey prey N/A 592819 unspecified role unspecified role bait bait c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 7B