IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sterner-2002-2 SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 sterner-2002-1 SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 sterner-2002-3 SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 sterner-2002-4 SALSA, a variant of yeast SAGA, contains truncated Spt7, which correlates with activated transcription yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast chromatography chromatography technology column chromatography chromatography western blot western blot Immuno blot Immuno blot Sterner DE., Belotserkovskaya R., Berger SL. This experiment was carried out with a yeast spt-217 strain, harbouring a truncated version of Spt7. Chromatographic preparations of complexes were subjected to Western blotting for three Spt subunits. berger@wistar.upenn.edu Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 spt3_yeast Protein SPT3 SPT3 YDR392W D9509.12 Positive regulator of Ty transcription protein protein yeast Saccharomyces cerevisiae Baker's yeast MMDKHKYRVEIQQMMFVSGEINDPPVETTSLIEDIVRGQVIEILLQSNKTAHLRGSRSILPEDVIFLIRHDKAKVNRLRTYLSWKDLRKNAKDQDASAGVASGTGNPGAGGEDDLKKAGGGEKDEKDGGNMMKVKKSQIKLPWELQFMFNEHPLENNDDNDDMDEDEREANIVTLKRLKMADDRTRNMTKEEYVHWSDCRQASFTFRKNKRFKDWSGISQLTEGKPHDDVIDILGFLTFEIVCSLTETALKIKQREQVLQTQKDKSQQSSQDNTNFEFASSTLHRKKRLFDGPENVINPLKPRHIEEAWRVLQTIDMRHRALTNFKGGRLSSKPIIM E0F60B61AB155C17 tra1_yeast Transcription-associated protein 1 TRA1 p400 kDa component of SAGA YHR099W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLTEQIEQFASRFRDDDATLQSRYSTLSELYDIMELLNSPEDYHFFLQAVIPLLLNQLKEVPISYDAHSPEQKLRNSMLDIFNRCLMNQTFQPYAMEVLEFLLSVLPKENEENGILCMKVLTTLFKSFKSILQDKLDSFIRIIIQIYKNTPNLINQTFYEAGKAEQGDLDSPKEPQADELLDEFSKNDEEKDFPSKQSSTEPRFENSTSSNGLRSSMFSFKILSECPITMVTLYSSYKQLTSTSLPEFTPLIMNLLNIQIKQQQEAREQAESRGEHFTSISTEIINRPAYCDFILAQIKATSFLAYVFIRGYAPEFLQDYVNFVPDLIIRLLQDCPSELSSARKELLHATRHILSTNYKKLFLPKLDYLFDERILIGNGFTMHETLRPLAYSTVADFIHNIRSELQLSEIEKTIKIYTGYLLDESLALTVQIMSAKLLLNLVERILKLGKENPQEAPRAKKLLMIIIDSYMNRFKTLNRQYDTIMKYYGRYETHKKEKAEKLKNSIQDNDKESEEFMRKVLEPSDDDHLMPQPKKEDINDSPDVEMTESDKVVKNDVEMFDIKNYAPILLLPTPTNDPIKDAFYLYRTLMSFLKTIIHDLKVFNPPPNEYTVANPKLWASVSRVFSYEEVIVFKDLFHECIIGLKFFKDHNEKLSPETTKKHFDISMPSLPVSATKDARELMDYLAFMFMQMDNATFNEIIEQELPFVYERMLEDSGLLHVAQSFLTSEITSPNFAGILLRFLKGKLKDLGNVDFNTSNVLIRLFKLSFMSVNLFPNINEVVLLPHLNDLILNSLKYSTTAEEPLVYFYLIRTLFRSIGGGRFENLYRSIKPILQVLLQSLNQMILTARLPHERELYVELCITVPVRLSVLAPYLPFLMKPLVFALQQYPDLVSQGLRTLELCIDNLTAEYFDPIIEPVIDDVSKALFNLLQPQPFNHAISHNVVRILGKLGGRNRQFLKPPTDLTEKTELDIDAIADFKINGMPEDVPLSVTPGIQSALNILQSYKSDIHYRKSAYKYLTCVLLLMTKSSAEFPTNYTELLKTAVNSIKLERIGIEKNFDLEPTVNKRDYSNQENLFLRLLESVFYATSIKELKDDAMDLLNNLLDHFCLLQVNTTLLNKRNYNGTFNIDLKNPNFMLDSSLILDAIPFALSYYIPEVREVGVLAYKRIYEKSCLIYGEELALSHSFIPELAKQFIHLCYDETYYNKRGGVLGIKVLIDNVKSSSVFLKKYQYNLANGLLFVLKDTQSEAPSAITDSAEKLLIDLLSITFADVKEEDLGNKVLENTLTDIVCELSNANPKVRNACQKSLHTISNLTGIPIVKLMDHSKQFLLSPIFAKPLRALPFTMQIGNVDAITFCLSLPNTFLTFNEELFRLLQESIVLADAEDESLSTNIQKTTEYSTSEQLVQLRIACIKLLAIALKNEEFATAQQGNIRIRILAVFFKTMLKTSPEIINTTYEALKGSLAENSKLPKELLQNGLKPLLMNLSDHQKLTVPGLDALSKLLELLIAYFKVEIGRKLLDHLTAWCRVEVLDTLFGQDLAEQMPTKIIVSIINIFHLLPPQADMFLNDLLLKVMLLERKLRLQLDSPFRTPLARYLNRFHNPVTEYFKKNMTLRQLVLFMCNIVQRPEAKELAEDFEKELDNFYDFYISNIPKNQVRVVSFFTNMVDLFNTMVITNGDEWLKKKGNMILKLKDMLNLTLKTIKENSFYIDHLQLNQSIAKFQALYLRFTELSERDQNPLLLDFIDFSFSNGIKASYSLKKFIFHNIIASSNKEKQNNFINDATLFVLSDKCLDARIFVLKNVINSTLIYEVATSGSLKSYLVEDKKPKWLELLHNKIWKNSNAILAYDVLDHHDLFRFELLQLSAIFIKADPEIIAEIKKDIIKFCWNFIKLEDTLIKQSAYLVTSYFISKFDFPIKVVTQVFVALLRSSHVEARYLVKQSLDVLTPVLHERMNAAGTPDTWINWVKRVMVENSSSQNNILYQFLISHPDLFFNSRDLFISNIIHHMNKITFMSNSNSDSHTLAIDLASLILYWENKTLEITNVNNTKTDSDGDVVMSDSKSDINPVEADTTAIIVDANNNSPISLHLREACTAFLIRYVCASNHRAIETELGLRAINILSELISDKHWTNVNVKLVYFEKFLIFQDLDSENILYYCMNALDVLYVFFKNKTKEWIMENLPTIQNLLEKCIKSDHHDVQEALQKVLQVIMKAIKAQGVSVIIEEESPGKTFIQMLTSVITQDLQETSSVTAGVTLAWVLFMNFPDNIVPLLTPLMKTFSKLCKDHLSISQPKDAMALEEARITTKLLEKVLYILSLKVSLLGDSRRPFLSTVALLIDHSMDQNFLRKIVNMSRSWIFNTEIFPTVKEKAAILTKMLAFEIRGEPSLSKLFYEIVLKLFDQEHFNNTEITVRMEQPFLVGTRVEDIGIRKRFMTILDNSLERDIKERLYYVIRDQNWEFIADYPWLNQALQLLYGSFNREKELSLKNIYCLSPPSILQEYLPENAEMVTEVNDLELSNFVKGHIASMQGLCRIISSDFIDSLIEIFYQDPKAIHRAWVTLFPQVYKSIPKNEKYGFVRSIITLLSKPYHTRQISSRTNVINMLLDSISKIESLELPPHLVKYLAISYNAWYQSINILESIQSNTSIDNTKIIEANEDALLELYVNLQEEDMFYGLWRRRAKYTETNIGLSYEQIGLWDKAQQLYEVAQVKARSGALPYSQSEYALWEDNWIQCAEKLQHWDVLTELAKHEGFTDLLLECGWRVADWNSDRDALEQSVKSVMDVPTPRRQMFKTFLALQNFAESRKGDQEVRKLCDEGIQLSLIKWVSLPIRYTPAHKWLLHGFQQYMEFLEATQIYANLHTTTVQNLDSKAQEIKRILQAWRDRLPNTWDDVNMWNDLVTWRQHAFQVINNAYLPLIPALQQSNSNSNINTHAYRGYHEIAWVINRFAHVARKHNMPDVCISQLARIYTLPNIEIQEAFLKLREQAKCHYQNMNELTTGLDVISNTNLVYFGTVQKAEFFTLKGMFLSKLRAYEEANQAFATAVQIDLNLAKAWAQWGFFNDRRLSEEPNNISFASNAISCYLQAAGLYKNSKIRELLCRILWLISIDDASGMLTNAFDSFRGEIPVWYWITFIPQLLTSLSHKEANMVRHILIRIAKSYPQALHFQLRTTKEDFAVIQRQTMAVMGDKPDTNDRNGRRQPWEYLQELNNILKTAYPLLALSLESLVAQINDRFKSTTDEDLFRLINVLLIDGTLNYNRLPFPRKNPKLPENTEKNLVKFSTTLLAPYIRPKFNADFIDNKPDYETYIKRLRYWRRRLENKLDRASKKENLEVLCPHLSNFHHQKFEDIEIPGQYLLNKDNNVHFIKIARFLPTVDFVRGTHSSYRRLMIRGHDGSVHSFAVQYPAVRHSRREERMFQLYRLFNKSLSKNVETRRRSIQFNLPIAIPLSPQVRIMNDSVSFTTLHEIHNEFCKKKGFDPDDIQDFMADKLNAAHDDALPAPDMTILKVEIFNSIQTMFVPSNVLKDHFTSLFTQFEDFWLFRKQFASQYSSFVFMSYMMMINNRTPHKIHVDKTSGNVFTLEMLPSRFPYERVKPLLKNHDLSLPPDSPIFHNNEPVPFRLTPNIQSLIGDSALEGIFAVNLFTISRALIEPDNELNTYLALFIRDEIISWFSNLHRPIIENPQLREMVQTNVDLIIRKVAQLGHLNSTPTVTTQFILDCIGSAVSPRNLARTDVNFMPWF AE3588676F5D5777 gcn5_yeast Histone acetyltransferase GCN5 YGR252W GCN5 ADA4 SWI9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQEGTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID 3200730DDC7EF70D spt20_yeast Transcription factor SPT20 SPT20 ADA5 YOL148C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSANSPTGNDPHVFGIPVNATPSNMGSPGSPVNVPPPMNPAVANVNHPVMRTNSNSNANEGTRTLTREQIQQLQQRQRLLLQQRLLEQQRKQQALQNYEAQFYQMLMTLNKRPKRLYNFVEDADSILKKYEQYLHSFEFHIYENNYKICAPANSRLQQQQKQPELTSDGLILTKNNETLKEFLEYVARGRIPDAIMEVLRDCNIQFYEGNLILQVYDHTNTVDVTPKENKPNLNSSSSPSNNNSTQDNSKIQQPSEPNSGVANTGANTANKKASFKRPRVYRTLLKPNDLTTYYDMMSYADNARFSDSIYQQFESEILTLTKRNLSLSVPLNPYEHRDMLEETAFSEPHWDSEKKSFIHEHRAESTREGTKGVVGHIEERDEFPQHSSNYEQLMLIMNERTTTITNSTFAVSLTKNAMEIASSSSNGVRGASSSTSNSASNTRNNSLANGNQVALAAAAAAAAVGSTMGNDNNQFSRLKFIEQWRINKEKRKQQALSANINPTPFNARISMTAPLTPQQQLLQRQQQALEQQQNGGAMKNANKRSGNNATSNNNNNNNNLDKPKVKRPRKNAKKSESGTPAPKKKRMTKKKQSASSTPSSTTMS 3D67937B65F9AA6A spt7_yeast Transcriptional activator SPT7 YBR0739 YBR081C SPT7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTERIPIKNYQRTNAKALLKLTEKLFNKNFFDLYLTSQQLVVLEYLLSISSEEDKLKAWDYFLKGNIALNVEKSFPLTQEEEHHGAVSPAVDTRSDDVSSQTIKDNNNTNTNTSISNENHVENEIEDKGDNAIANEDNFVNNDESDNVEEDLFKLDLEDLKQQISGTRFIGNLSLKIRYVLWQCAIDYIYCDRNEFGDENDTEYTLLDVEEKEEEEIGKNEKPQNKEGISKFAEDEDYDDEDENYDEDSTDVKNVDDPPKNLDSISSSNIEIDDERRLVLNISISKETLSKLKTNNVEEIMGNWNKIYHSFEYDKETMIKRLKLEESDKMIEKGKKKRSRSDLEAATDEQDRENTNDEPDTNQKLPTPEGSTFSDTGNKRPKQSNLDLTVNLGIENLSLKHLLSSIQQKKSQLGISDYELKHLIMDVRKNRSKWTSDERIGQEELYEACEKVVLELRNYTEHSTPFLNKVSKREAPNYHQIIKKSMDLNTVLKKLKSFQYDSKQEFVDDIMLIWKNCLTYNSDPSHFLRGHAIAMQKKSLQLIRMIPNITIRNRADLEKEIEDMEKDKDYELDEEEEVAGSGRKGLNMGAHMLAKENGKVSEKDSSKTVKDEAPTNDDKLTSVIPEGEKEKDKTASSTVTVHENVNKNEIKENGKNEEQDMVEESSKTEDSSKDADAAKKDTEDGLQDKTAENKEAGENNEEEEDDDDEDEDEDMVDSQSYLLEKDDDRDDLEISVWKTVTAKVRAEICLKRTEYFKNGKLNSDSEAFLKNPQRMKRFDQLFLEYKEQKALESYRQKIEQNSIMKNGFGTVLKQEDDDQLQFHNDHSLNGNEAFEKQPNDIELDDTRFLQEYDISNAIPDIVYEGVNTKTLDKMEDASVDRMLQNGINKQSRFLANKDLGLTPKMNQNITLIQQIRHICHKISLIRMLQSPLSAQNSRSNPNAFLNNHIYNYTIIDDSLDIDPVSQLPTHDYKNNRELIWKFMHKNISKVAMANGFETAHPSAINMLTEIAGDYLSNLIKTLKLHHETNSLNRGTNVEMLQTTLLENGINRPDDLFSYVESEFGKKTKKLQDIKQKLESFLRALLRPTLQELSERNFEDESQSFFTGDFASELTGEDFFGFRELGLEKEFGVLSSSVPLQLLTTQFQTVDGETKVQAKKIQPEESDSIVYKKITKGMLDAGSFWNTLLPLLQKDYERSKAYIAKQSKSSANDKTSMTSTEDNSFALLEEDQFVSKKTATKARLPPTGKISTTYKKKPIASAFILPEEDLENDVKADPTTTVNAKVGAENDGDSSLFLRTPQPLDPLDMDDAFDDTNMGSNSSFSLSLPRLNQ 083B63624669244F ada2_yeast Transcriptional adapter 2 ADA2 D9461.33 YDR448W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKFHCDVCSADCTNRVRVSCAICPEYDLCVPCFSQGSYTGKHRPYHDYRIIETNSYPILCPDWGADEELQLIKGAQTLGLGNWQDIADHIGSRGKEEVKEHYLKYYLESKYYPIPDITQNIHVPQDEFLEQRRHRIESFRERPLEPPRKPMASVPSCHEVQGFMPGRLEFETEFENEAEGPVKDMVFEPDDQPLDIELKFAILDIYNSRLTTRAEKKRLLFENHLMDYRKLQAIDKKRSKEAKELYNRIKPFARVMTAQDFEEFSKDILEELHCRARIQQLQEWRSNGLTTLEAGLKYERDKQARISSFEKFGASTAASLSEGNSRYRSNSAHRSNAEYSQNYSENGGRKKNMTISDIQHAPDYALLSNDEQQLCIQLKILPKPYLVLKEVMFRELLKTGGNLSKSACRELLNIDPIKANRIYDFFQSQNWM 9637E1EDBBED0AC3 spt8_yeast Transcription factor SPT8 L2144 YLR055C SPT8 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDEVDDILINNQVVDDEEDDEEMLSGLENDSKQDLEGNDDGGEDEEDDDDDDEDDDDDEDEREDDDEQEDDDGEDDAARMDKTATPTNEHQHDEQKAAAAGAGGAGDSGDAVTKIGSEDVKLSDVDGGVGSREASSSTHEASANGEVYEYYKHMLNAAQIADSYNIYPTAAIPIQTHVNALAVSRGLKYLFLGGSDGYIRKYDLLNTLEGKLSLTILQKHSLAESIQNAGILQSYWENEIPQKKSEMKLSANKTDYEPKVSPVHSLEVQSECLFILSGLQNGGITMQGVRYMEGSIAHYFKGRNGHTQIVNILRLNGQEDRFLSGSWDKRLLEWDLQTGDIVNEFKKSRSELSSLEMRPLYSSVDVSGNVNSGKENENADDDMDSLFGDEDEDEKQDAGNEPVETGDGSNGEENKEQISEESLNIVYDESVFMTSGLNGSVHIWDRRMTQSPALSLERGAGVPPWCLSACWGVDGDHVYAGRRNACVEQFDLKMPSKPIHNLKLPSISGPVSCVKAMPNNKHLLCASRDNIRLYNVEIAVDASNSTTKSSKVPFLIVPGHHGGIISNLYLDPTSRFIISTSGNRGWQGNSTDTTLIYDIDLE DDE88155E7638B46 ngg1_yeast Chromatin-remodeling complexes subunit NGG1 NGG1 ADA3 YDR176W YD9395.09 SWI7 Transcriptional adapter 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRHGRRGKLPKGEKLPKKEGGDNTPSKLLSSMLKTLDLTFERDIGMLNGKSVRSIPNKKTLLELQSQLDSLNEILGTIARGDQETIEALRKIRDSKNEKQANDEKQETSNADGQHESSTATEETNIIDKGVQSPPKPPPSNEISGTIENDVESIKQAADNMAKEEINEDKDLQVHRDQPREKRPFDSETENRATENENTQRPDNKKQKIDVDKMENDPTVKNPKSEFVVSQTLPRAAAALGLFNEEGLESTGEDFLKKKYNVASYPTNDLKDLLPGELPDMDFSHPKPTNQIQFNTFLAFVENFFKDLSDDNLKFLKMKYIIPDSLQFDKTYDPEVNPFIIPKLGPLYTDVWFKDENDKNSAYKKPSPYSNDASTILPKKSANELDDNALESGSISCGPLLSRLLSAVLKDDNDKSELQSSKIIRDGGLPRTGGEDDIQSFRNNNNDTVDMTLSQENGPSVQTPDNDIDEEASFQAKLAENKGSNGGTTSTLPQQIGWITNGINLDYPTFEERLKRELKYVGIYMNLPKDENNPNSDDPDWVTGREDDEISAELRELQGTLKQVTKKNQKRKAQLIPLVERQLAWQEYSSILEDLDKQIDQAYVKRIRVPKKRKKHHTAASNNVNTGTTSQIAQQKAANSSLKSLLDKRQRWINKIGPLFDKPEIMKRIPNESVFKDMDQEEDEDEADVFAQNTNKDVELN 489016BFD1C095D4 taf6_yeast Transcription initiation factor TFIID subunit 6 TAF6 TAF60 YGL112C G2985 TBP-associated factor 6 TBP-associated factor 60 kDa protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTQQQSYTIWSPQDTVKDVAESLGLENINDDVLKALAMDVEYRILEIIEQAVKFKRHSKRDVLTTDDVSKALRVLNVEPLYGYYDGSEVNKAVSFSKVNTSGGQSVYYLDEEEVDFDRLINEPLPQVPRLPTFTTHWLAVEGVQPAIIQNPNLNDIRVSQPPFIRGAIVTALNDNSLQTPVTSTTASASVTDTGASQHLSNVKPGQNTEVKPLVKHVLSKELQIYFNKVISTLTAKSQADEAAQHMKQAALTSLRTDSGLHQLVPYFIQFIAEQITQNLSDLQLLTTILEMIYSLLSNTSIFLDPYIHSLMPSILTLLLAKKLGGSPKDDSPQEIHEFLERTNALRDFAASLLDYVLKKFPQAYKSLKPRVTRTLLKTFLDINRVFGTYYGCLKGVSVLEGESIRFFLGNLNNWARLVFNESGITLDNIEEHLNDDSNPTRTKFTKEETQILVDTVISALLVLKKDLPDLYEGKGEKVTDEDKEKLLERCGVTIGFHILKRDDAKELISAIFFGE 14B590E02C16F289 hfi1_yeast Transcriptional coactivator HFI1/ADA1 YPL254W HFI1 ADA1 SUP110 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAIQSPAPKPLQPTYPAASPASTNAYMKPGLIGSPAVSNHTEPNNGNNETAEPQGPNQRIDLGAMIEELTSLLGKESWTKYAQIISLFILGKLSRKELSNELELVFSPSAASLEKSNTNHHHSLVRLHNQLLLGIFANSLRENPLGRNGNESSWGFGNGSNNPNNKLKRINKHNSQIEVYKKIVMSLPLNDRNRLKMITKEAGKRGFIFCSVFQARLNNIPKIPIVTNPESLKRVKSNNLKTPLEWSQDIMNGFNVPLASESHSLPDTDSFYLRMVGIAREHGLVGTVDARCVELISLALDQYLKNIIEFTIDTVRYRRKKYSDYYDLNESGLYKSVSEMAADKRDAKIKQLDDDKNEDECADEAKSINNGNNSSKDDIGDISMSSITKAGEAVNEELHENRTISLTNEDIYDSLSIFPNLVEPSGSYYALTNLGLVNDDELVDMKSNIDDLPDFLNEKPTFTPLDERNVGTRHELNWLIKGILTED B3ACF3C6101AB541 taf5_yeast Transcription initiation factor TFIID subunit 5 TAF5 TAF90 YBR198C YBR1410 TBP-associated factor 5 TBP-associated factor 90 kDa TAFII-90 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQKQSTNQNQNGTHQPQPVKNQRTNNAAGANSGQQPQQQSQGQSQQQGRSNGPFSASDLNRIVLEYLNKKGYHRTEAMLRAESGRTLTPQNKQSPANTKTGKFPEQSSIPPNPGKTAKPISNPTNLSSKRDAEGGIVSSGRLEGLNAPENYIRAYSMLKNWVDSSLEIYKPELSYIMYPIFIYLFLNLVAKNPVYARRFFDRFSPDFKDFHGSEINRLFSVNSIDHIKENEVASAFQSHKYRITMSKTTLNLLLYFLNENESIGGSLIISVINQHLDPNIVESVTAREKLADGIKVLSDSENGNGKQNLEMNSVPVKLGPFPKDEEFVKEIETELKIKDDQEKQLNQQTAGDNYSGANNRTLLQEYKAMNNEKFKDNTGDDDKDKIKDKIAKDEEKKESELKVDGEKKDSNLSSPARDILPLPPKTALDLKLEIQKVKESRDAIKLDNLQLALPSVCMYTFQNTNKDMSCLDFSDDCRIAAAGFQDSYIKIWSLDGSSLNNPNIALNNNDKDEDPTCKTLVGHSGTVYSTSFSPDNKYLLSGSEDKTVRLWSMDTHTALVSYKGHNHPVWDVSFSPLGHYFATASHDQTARLWSCDHIYPLRIFAGHLNDVDCVSFHPNGCYVFTGSSDKTCRMWDVSTGDSVRLFLGHTAPVISIAVCPDGRWLSTGSEDGIINVWDIGTGKRLKQMRGHGKNAIYSLSYSKEGNVLISGGADHTVRVWDLKKATTEPSAEPDEPFIGYLGDVTASINQDIKEYGRRRTVIPTSDLVASFYTKKTPVFKVKFSRSNLALAGGAFRP B42315B8C752D0B6 taf12_yeast Transcription initiation factor TFIID subunit 12 TAF12 TAF61 TAF68 YDR145W YD8358.02 TBP-associated factor 12 TBP-associated factor 61 kDa TAFII-61 TAFII-68 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSNPENSGVNANNNTGTGNADAITGAQQNMVLQPRQLQEMAAKFRTLLTEARNVGETTPRGKELMFQAAKIKQVYDALTLNRRRQQAAQAYNNTSNSNSSNPASIPTENVPNSSQQQQQQQQQTRNNSNKFSNMIKQVLTPEENQEYEKLWQNFQVRHTSIKEKETYLKQNIDRLEQEINKQTDEGPKQQLQEKKIELLNDWKVLKIEYTKLFNNYQNSKKTFYVECARHNPALHKFLQESTQQQRVQQQRVQQQQQQQQQQQQQQQQQQQQQQQRQGQNQRKISSSNSTEIPSVTGPDALKSQQQQQNTITATNNPRGNVNTSQTEQSKAKVTNVNATASMLNNISSSKSAIFKQTEPAIPISENISTKTPAPVAYRSNRPTITGGSAMNASALNTPATTKLPPYEMDTQRVMSKRKLRELVKTVGIDEGDGETVIDGDVEELLLDLADDFVTNVTAFSCRLAKHRKSDNLEARDIQLHLERNWNIRIPGYSADEIRSTRKWNPSQNYNQKLQSITSDKVAAAKNNGNNVASLNTKK A8FC84E46F8370D3 ada2-ada3-6 Purification of the yeast SALSA complex 671535 N/A 671539 unspecified role unspecified role prey prey N/A 671540 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey N/A 671543 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 1 B SALSA, as compared to SAGA, lacks Spt8 and also contains a truncated form of Spt7. ada2-ada3-7 Purification of the yeast SAGA complex 671535 N/A 671540 unspecified role unspecified role prey prey N/A 671543 unspecified role unspecified role prey prey N/A 671539 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671541 unspecified role unspecified role prey prey N/A 671545 unspecified role unspecified role prey prey association association false false false 1 B ada2-ada3-8 Ada2-2FLAG coimmunoprecipitates SAGA complex proteins from extracts of cells expressing wild-type Spt7 671535 N/A 671542 unspecified role unspecified role prey prey N/A 671546 unspecified role unspecified role prey prey N/A 671547 unspecified role unspecified role prey prey N/A 671540 unspecified role unspecified role prey prey N/A 671545 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey N/A 671543 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671539 unspecified role unspecified role prey prey N/A 671549 unspecified role unspecified role prey prey N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5 A These proteins apart Spt8 are coimmunoprecipitated with Ada-2-2FLAG in cells expressing Spt7Delta213C (aa 1- 1119) (like spt7-217), indicating a structural and functional relationship between the Spt7 C-terminus and the participitation of Spt8 in the complex. ada2-ada3-9 Ada2-2FLAG coimmunoprecipitates SALSA complex proteins from extracts of cells expressing Spt7Delta213C 671535 N/A 671543 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false N/A 671546 unspecified role unspecified role prey prey N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671540 unspecified role unspecified role prey prey N/A 671549 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671547 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey N/A 671539 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey association association false false false 5 A Spt8 is not coimmunoprecipitated with Ada-2-2FLAG in cells expressing Spt7Delta213C (aa 1- 1119) (like spt7-217), indicating a structural and functional relationship between the Spt7 C-terminus and the participitation of Spt8 in the complex. ada2-ada3-10 Ada2-2FLAG coimmunoprecipitates fewer SALSA complex proteins from extracts of cells expressing Spt7Delta400C or no Spt7 671535 N/A 671546 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5 A These proteins were coimmunoprecipitated from extracts of cells expressing Spt7Delta400C (aa 1-932) or Spt7 deletion mutants. spt3-ada3 Spt3-13Myc coimmunoprecipitates Ada1, Ada3 and Taf60 from yeast cell extracts 671535 N/A 671547 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671546 unspecified role unspecified role prey prey N/A 671539 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5 B spt20-ada1 Spt20-13Myc coimmunoprecipitates Ada1, Ada3 and Taf60 from yeast cell extracts 671535 N/A 671546 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671547 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 5 B spt8-ada1 Spt8-13Myc coimmunoprecipitates Ada1, Ada3 and Taf60 in the presence of wild-type Spt7 671535 N/A 671547 unspecified role unspecified role prey prey N/A 671546 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671545 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false SAGA/SALSA complex proteins could be pulled down by Spt8 in the presence of wild-type Spt7 but not Delta213C Spt7 (aa 1-1119). 5 B ada2-ada3-1 Purification of the yeast SAGA complex 671536 N/A 671540 unspecified role unspecified role prey prey N/A 671549 unspecified role unspecified role prey prey N/A 671539 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671547 unspecified role unspecified role prey prey N/A 671545 unspecified role unspecified role prey prey N/A 671546 unspecified role unspecified role prey prey N/A 671543 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671550 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey association association false false false 1 B ada2-ada3-5 Purification of the yeast SALSA complex 671536 N/A 671543 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 671544 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 671539 unspecified role unspecified role prey prey N/A 671550 unspecified role unspecified role prey prey N/A 671540 unspecified role unspecified role prey prey N/A 671549 unspecified role unspecified role prey prey N/A 671541 unspecified role unspecified role prey prey N/A 671546 unspecified role unspecified role prey prey N/A 671547 unspecified role unspecified role prey prey N/A 671548 unspecified role unspecified role prey prey N/A 671542 unspecified role unspecified role prey prey association association false false false 1 B SALSA, as compared to SAGA, lacks Spt8 and also contains a truncated form of Spt7. spt3-spt8 SAGA subunits Spt20, Spt8 and Spt3 elute in the same fractions in a yeast strain expressing wild-type Spt7 671537 N/A 671542 unspecified role unspecified role neutral component neutral component N/A 671545 unspecified role unspecified role neutral component neutral component N/A 671539 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4 spt3-spt20 SALSA subunits Spt20 and Spt3 elute in the same fractions in a yeast strain expressing wild-type Spt7 671537 N/A 671542 unspecified role unspecified role neutral component neutral component N/A 671539 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4 spt3-spt8-1 SAGA subunits Spt20, Spt8 and Spt3 elute in the same fractions in a yeast Spt7-217 strain 671538 N/A 671539 unspecified role unspecified role neutral component neutral component N/A 671542 unspecified role unspecified role neutral component neutral component N/A 671545 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4 spt3-spt20-1 SALSA subunits Spt20, Spt8 and Spt3 elute in the same fractions in a yeast Spt7-217 strain 671538 N/A 671542 unspecified role unspecified role neutral component neutral component N/A 671539 unspecified role unspecified role neutral component neutral component physical association physical association false false false 4