IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ reiss-2001-1 Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. mouse-p6 Mus musculus P6 cells p6 Mouse P6 cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu Clin. Cancer Res. (1078-0432) 2001 reiss-2001-2 Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu Clin. Cancer Res. (1078-0432) 2001 igf1r_human Insulin-like growth factor 1 receptor Insulin-like growth factor I receptor IGF1R protein protein human Homo sapiens Human MKSGSGGGSPTSLWGLLFLSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAVSNNYIVGNKPPKECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSTCGKRACTENNECCHPECLGSCSAPDNDTACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRDFCANILSAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERKRRDVMQVANTTMSSRSRNTTAADTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTGYENFIHLIIALPVAVLLIVGGLVIMLYVFHRKRNNSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTYSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSIKEEMEPGFREVSFYYSEENKLPEPEELDLEPENMESVPLDPSASSSSLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC igf1r-igf1r FLAG-486/STOP coimmunoprecipitates the IGF-IR proreceptor, IGF-IRalpha and IGR-IRbeta in P6 lysates 669033 N/A 669035 unspecified role unspecified role prey prey c-terminal IGF-IRbeta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 669035 unspecified role unspecified role prey prey region IGF-IRalpha sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 669035 unspecified role unspecified role prey prey N/A 669035 unspecified role unspecified role bait bait region 486/STOP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 3 A, B P6 cells constitutively overexpress P08069. P08069 transfection The fraction of IGF-IR that coprecipitates with 486/STOP is very small. P6 cells, which overecpress IGF-IR, have about 5 E5 receptors/cell. Densitometric measurements of blots under different conditions indicate that the fraction of IGF-IR coprecipitating with 486/STOP is less than 5%. igf1r-igf1r-1 FLAG-486/STOP coimmunoprecipitates IGR-IRbeta from P6 lysates 669034 N/A 669035 unspecified role unspecified role prey prey c-terminal IGF-IRbeta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false mouse-p6 Mus musculus P6 cells p6 Mouse P6 cells N/A 669035 unspecified role unspecified role bait bait region 486/STOP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false mouse-r- Mouse R- cells (embryonic fibroblasts lacking type-1 IGF receptor) r- Mouse R- cells (embryonic fibroblasts lacking the type-1 IGF receptor ) Mouse embryonic fibroblasts lacking the type-1 IGF receptor. physical association physical association false false false 8 P6 cells constitutively overexpress P08069. P08069 transfection