UniProt UniProt http://www.uniprot.org manning-1999-1 Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Manning BD., Barrett JG., Wallace JA., Granok H., Snyder M. michael.snyder@yale.edu J. Cell Biol. 144 (1219-1233) 1999 kar3_yeast Kinesin-like protein KAR3 KAR3 YPR141C P9659.16 Nuclear fusion protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MESLPRTPTKGRSTQHLSTPSPKNDILAMNGHKRRNTTTPPPKHTLLKPQRTDIHRHSLASQSRISMSPNRELLKNYKGTANLIYGNQKSNSGVTSFYKENVNELNRTQAILFEKKATLDLLKDELTETKEKINAVNLKFETLREEKIKIEQQLNLKNNELISIKEEFLSKKQFMNEGHEIHLKQLAASNKKELKQMENEYKTKIEKLKFMKIKQFENERASLLDKIEEVRNKITMNPSTLQEMLNDVEQKHMLEKEEWLTEYQSQWKKDIELNNKHMQEIESIKKEIENTLKPELAEKKKLLTEKRNAYEAIKVKVKEKEEETTRLRDEVALKQKTNLETLEKIKELEEYIKDTELGMKELNEILIKEETVRRTLHNELQELRGNIRVYCRIRPALKNLENSDTSLINVNEFDDNSGVQSMEVTKIQNTAQVHEFKFDKIFDQQDTNVDVFKEVGQLVQSSLDGYNVCIFAYGQTGSGKTFTMLNPGDGIIPSTISHIFNWINKLKTKGWDYKVNCEFIEIYNENIVDLLRSDNNNKEDTSIGLKHEIRHDQETKTTTITNVTSCKLESEEMVEIILKKANKLRSTASTASNEHSSRSHSIFIIHLSGSNAKTGAHSYGTLNLVDLAGSERINVSQVVGDRLRETQNINKSLSCLGDVIHALGQPDSTKRHIPFRNSKLTYLLQYSLTGDSKTLMFVNISPSSSHINETLNSLRFASKVNSTRLVSRK vik1_yeast Spindle pole body-associated protein VIK1 YPL253C VIK1 Vegetative interaction with KAR3 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MASQQNKHAFLSKNRIFHNPDNVSSSKSRNLMDITNTTNTMNGSRPSSMKSSLALPPVKDSFPSVSRSASLNINMSKIKDLKDRQDKIRFQRHTLRTQLIECEREIKTIKFRDLNKSRFELYKKKSKQAKYLKQVRDLTQNLNSKDGERADLIKKNKSALATLQAELDQNLILKRQESQELYNNKLIFWENELQIMENVEPDHEITEEISQLKKTLQELNINWANLQKQNLERQVNHESQLRKDFIAFKEAKLKSMENLTNKHRELLDQIATLQSESEKLHKEIMDIDRQAEYSEQNISEINENIKQLELANNPLISKSLQNSQDLEHLQNQMENLKEMASKQEKFYNDTYNTVEKELLRSRRLENSIIEQKGTMRCYAYVMEQNLPENLLFDYENGVITQGLSEHVYKFNRVIPHLKVSEDKFFTQEYSVYHDMCLNQKKNFNLISLSTTPHGSLRESLIKFLAEKDTIYQKQYVITLQFVFLSDDEFSQDMLLDYSHNDKDSIKLKFEKHSISLDSKLVIIENGLEDLPLNFSCDEHPNLPHSGMGIIKVQFFPRDSKSDGNNDPVPVDFYFIELNNLKSIEQFDKSIFKKESCETPIALVLKKLISDTKSFFLLNLNDSKNVNKLLTISEEVQTQLCKRKKKLT kar3-vik1-2 Vik1p coimmunoprecipitates with Kar3p 682920 N/A 682921 unspecified role unspecified role bait bait N/A 682922 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig: 3B The interaction is very stable since it was performed in presence of 1M NaCl, and it does not need the presence of Cik1.