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<entrySet level="2" version="5" minorVersion="4" xsi:schemaLocation="http://psi.hupo.org/mi/mif http://psidev.sourceforge.net/mi/rel25/src/MIF254.xsd" xmlns="http://psi.hupo.org/mi/mif" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
    <entry>
        <source releaseDate="2006-09-22+01:00">
            <names>
                <shortLabel>UniProt</shortLabel>
                <fullName>UniProt</fullName>
            </names>
            <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0486" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-3989247" refType="identity" refTypeAc="MI:0356"/>
            </xref>
            <attributeList>
                <attribute name="url" nameAc="MI:0614">http://www.uniprot.org</attribute>
            </attributeList>
        </source>
        <experimentList>
            <experimentDescription id="370248">
                <names>
                    <shortLabel>anderson-1998-1</shortLabel>
                    <fullName>The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA.</fullName>
                </names>
                <bibref>
                    <xref>
                        <primaryRef db="pubmed" dbAc="MI:0446" id="9851972" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </bibref>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-934088" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-19628" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <hostOrganismList>
                    <hostOrganism ncbiTaxId="4932">
                        <names>
                            <shortLabel>yeasx</shortLabel>
                            <fullName>Saccharomyces cerevisiae (Baker's yeast)</fullName>
                            <alias typeAc="MI:1041" type="synonym">Baker's yeast</alias>
                        </names>
                    </hostOrganism>
                </hostOrganismList>
                <interactionDetectionMethod>
                    <names>
                        <shortLabel>pull down</shortLabel>
                        <fullName>pull down</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </interactionDetectionMethod>
                <participantIdentificationMethod>
                    <names>
                        <shortLabel>western blot</shortLabel>
                        <fullName>western blot</fullName>
                        <alias typeAc="MI:0303" type="go synonym">Immuno blot</alias>
                        <alias typeAc="MI:1041" type="synonym">Immuno blot</alias>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                    </xref>
                </participantIdentificationMethod>
                <attributeList>
                    <attribute name="author-list" nameAc="MI:0636">Anderson J., Phan L., Cuesta R., Carlson BA., Pak M., Asano K., Bjork GR., Tamame M., Hinnebusch AG.</attribute>
                    <attribute name="journal" nameAc="MI:0885">Genes Dev. (0890-9369)</attribute>
                    <attribute name="publication year" nameAc="MI:0886">1998</attribute>
                    <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
                    <attribute name="imex curation" nameAc="MI:0959"/>
                    <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
                </attributeList>
            </experimentDescription>
        </experimentList>
        <interactorList>
            <interactor id="370249">
                <names>
                    <shortLabel>trm61_yeast</shortLabel>
                    <fullName>tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
                    <alias typeAc="MI:0301" type="gene name">GCD14</alias>
                    <alias typeAc="MI:0305" type="locus name">YJL125C</alias>
                    <alias typeAc="MI:0306" type="orf name">J0710</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">TRM61</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">tRNA(m1A58)-methyltransferase subunit TRM61</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">General control non-derepressible protein 14</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P46959" version="SP_57" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR029063" version="SP_168"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5ERG" version="SP_168"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_012410.1"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031515" secondary="tRNA (m1A) methyltransferase complex" version="SP_168"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6VW61" version="SP_87" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5EQJ" version="SP_168"/>
                    <secondaryRef db="sgd" dbAc="MI:0484" id="S000003661"/>
                    <secondaryRef db="ensemblfungi" id="YJL125C" version="SP_168" refType="gene"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-7416" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="dip" dbAc="MI:0465" id="DIP-5421N"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030488" secondary="tRNA methylation" version="SP_168"/>
                    <secondaryRef db="ensemblfungi" id="YJL125C_mRNA" version="SP_168" refType="transcript"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR014816" version="SP_168"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0016429" secondary="tRNA (adenine-N1-)-methyltransferase activity" version="SP_168"/>
                    <secondaryRef db="ensemblfungi" id="YJL125C" version="SP_168" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="P46959"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" secondary="nucleus" version="SP_168"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="559292">
                    <names>
                        <shortLabel>yeast</shortLabel>
                        <fullName>Saccharomyces cerevisiae</fullName>
                        <alias typeAc="MI:1041" type="synonym">Baker's yeast</alias>
                    </names>
                </organism>
                <sequence>MSTNCFSGYKDLIKEGDLTLIWVSRDNIKPVRMHSEEVFNTRYGSFPHKDIIGKPYGSQIAIRTKGSNKFAFVHVLQPTPELWTLSLPHRTQIVYTPDSSYIMQRLNCSPHSRVIEAGTGSGSFSHAFARSVGHLFSFEFHHIRYEQALEEFKEHGLIDDNVTITHRDVCQGGFLIKKGDTTSYEFGNNETAASLNANVVFLDLPAPWDAIPHLDSVISVDEKVGLCCFSPCIEQVDKTLDVLEKYGWTDVEMVEIQGRQYESRRQMVRSLNDALERLRDIKRHKLQGVERRKRMFNNTIDSNDEKVGKRNEDGVPLTEKAKFNPFGKGSRIKEGDSNYKWKEVTKMEAEIKSHTSYLTFAFKVVNRSRDDEKVNEILRSTEK</sequence>
            </interactor>
            <interactor id="370250">
                <names>
                    <shortLabel>trm6_yeast</shortLabel>
                    <fullName>tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
                    <alias typeAc="MI:0301" type="gene name">GCD10</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">TIF33</alias>
                    <alias typeAc="MI:0305" type="locus name">YNL062C</alias>
                    <alias typeAc="MI:0306" type="orf name">N2422</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">TRM6</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">tRNA(m1A58)-methyltransferase subunit TRM6</alias>
                    <alias typeAc="MI:0302" type="gene name synonym">General control non-derepressible protein 10</alias>
                </names>
                <xref>
                    <primaryRef db="uniprotkb" dbAc="MI:0486" id="P41814" version="SP_57" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0003723" secondary="RNA binding" version="SP_170"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5EQJ" version="SP_170"/>
                    <secondaryRef db="ensemblfungi" id="YNL062C" version="SP_170" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017423" version="SP_170"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0005634" secondary="nucleus" version="SP_170"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0030488" secondary="tRNA methylation" version="SP_170"/>
                    <secondaryRef db="go" dbAc="MI:0448" id="GO:0031515" secondary="tRNA (m1A) methyltransferase complex" version="SP_170"/>
                    <secondaryRef db="uniprotkb" dbAc="MI:0486" id="D6W1B8" version="SP_85" refType="secondary-ac" refTypeAc="MI:0360"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8995" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="mint" dbAc="MI:0471" id="P41814"/>
                    <secondaryRef db="ensemblfungi" id="YNL062C" version="SP_170" refType="gene"/>
                    <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5ERG" version="SP_170"/>
                    <secondaryRef db="refseq" dbAc="MI:0481" id="NP_014337.3"/>
                    <secondaryRef db="sgd" dbAc="MI:0484" id="S000005006"/>
                    <secondaryRef db="dip" dbAc="MI:0465" id="DIP-3842N"/>
                    <secondaryRef db="ensemblfungi" id="YNL062C_mRNA" version="SP_170" refType="transcript"/>
                </xref>
                <interactorType>
                    <names>
                        <shortLabel>protein</shortLabel>
                        <fullName>protein</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactorType>
                <organism ncbiTaxId="559292">
                    <names>
                        <shortLabel>yeast</shortLabel>
                        <fullName>Saccharomyces cerevisiae</fullName>
                        <alias typeAc="MI:1041" type="synonym">Baker's yeast</alias>
                    </names>
                </organism>
                <sequence>MNALTTIDFNQHVIVRLPSKNYKIVELKPNTSVSLGKFGAFEVNDIIGYPFGLTFEIYYDGEEVSSDENRDSKPKNKIPIGKVRLLSQEIKDVNNDKDDGQSEPPLSIKEKSVSLELSSIDSSATNQNLVNMGSKAQELTVEEIEKMKQESLSSKEIIDKIIKSHKSFHNKTVYSQEKYVNRKKQKFAKYFTVEYLSSSNLLQFLIDKGDIQRVLDMSQESMGMLLNLANIQSEGNYLCMDETGGLLVYFLLERMFGGDNESKSKGKVIVIHENEHANLDLLKFANYSEKFIKEHVHTISLLDFFEPPTLQEIQSRFTPLPKEEARALKGGKKNSYYRKLRWYNTQWQILELTGEFLYDGLVMATTLHLPTLVPKLAEKIHGSRPIVCYGQFKETLLELAHTLYSDLRFLAPSILETRCRPYQSIRGKLHPLMTMKGGGGYLMWCHRVIPAPEPVSENATAADSSEKLAEHGAKKQKI</sequence>
            </interactor>
        </interactorList>
        <interactionList>
            <interaction imexId="IM-19628-1" id="370251">
                <names>
                    <shortLabel>gcd10-gcd14-1</shortLabel>
                </names>
                <xref>
                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-934090" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="imex" dbAc="MI:0670" id="IM-19628-1" refType="imex-primary" refTypeAc="MI:0662"/>
                </xref>
                <experimentList>
                    <experimentRef>370248</experimentRef>
                </experimentList>
                <participantList>
                    <participant id="370252">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-934094" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>370249</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>prey</shortLabel>
                                    <fullName>prey</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                    </participant>
                    <participant id="370253">
                        <names>
                            <shortLabel>N/A</shortLabel>
                        </names>
                        <xref>
                            <primaryRef db="intact" dbAc="MI:0469" id="EBI-934091" refType="identity" refTypeAc="MI:0356"/>
                        </xref>
                        <interactorRef>370250</interactorRef>
                        <biologicalRole>
                            <names>
                                <shortLabel>unspecified role</shortLabel>
                                <fullName>unspecified role</fullName>
                            </names>
                            <xref>
                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                            </xref>
                        </biologicalRole>
                        <experimentalRoleList>
                            <experimentalRole>
                                <names>
                                    <shortLabel>bait</shortLabel>
                                    <fullName>bait</fullName>
                                </names>
                                <xref>
                                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                </xref>
                            </experimentalRole>
                        </experimentalRoleList>
                        <featureList>
                            <feature id="370254">
                                <names>
                                    <shortLabel>region</shortLabel>
                                </names>
                                <xref>
                                    <primaryRef db="intact" dbAc="MI:0469" id="EBI-934092" refType="identity" refTypeAc="MI:0356"/>
                                </xref>
                                <featureType>
                                    <names>
                                        <shortLabel>his tag</shortLabel>
                                        <fullName>his tag</fullName>
                                        <alias typeAc="MI:0303" type="go synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">6-His-tag</alias>
                                        <alias typeAc="MI:0303" type="go synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Hexa-His-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">Histidine-tag</alias>
                                        <alias typeAc="MI:1041" type="synonym">6-His-tag</alias>
                                    </names>
                                    <xref>
                                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                                    </xref>
                                </featureType>
                                <featureRangeList>
                                    <featureRange>
                                        <startStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </startStatus>
                                        <endStatus>
                                            <names>
                                                <shortLabel>undetermined</shortLabel>
                                                <fullName>undetermined sequence position</fullName>
                                                <alias typeAc="MI:1041" type="synonym">undetermined</alias>
                                            </names>
                                            <xref>
                                                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0339" refType="identity" refTypeAc="MI:0356"/>
                                                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                                                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-448295" refType="identity" refTypeAc="MI:0356"/>
                                            </xref>
                                        </endStatus>
                                        <isLink>false</isLink>
                                    </featureRange>
                                </featureRangeList>
                            </feature>
                        </featureList>
                    </participant>
                </participantList>
                <interactionType>
                    <names>
                        <shortLabel>physical association</shortLabel>
                        <fullName>physical association</fullName>
                    </names>
                    <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
                    </xref>
                </interactionType>
                <modelled>false</modelled>
                <intraMolecular>false</intraMolecular>
                <negative>false</negative>
                <attributeList>
                    <attribute name="figure legend" nameAc="MI:0599">5a</attribute>
                </attributeList>
            </interaction>
        </interactionList>
    </entry>
</entrySet>
