IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ albrecht-1998-2 Homodimers and heterodimers of Pho1-type phosphorylase isoforms in Solanum tuberosum L. as revealed by sequence-specific antibodies. ecoli Escherichia coli (strain K12) affinity techniques affinity technology affinity techniques western blot western blot Immuno blot Immuno blot Albrecht T., Greve B., Pusch K., Kossmann J., Buchner P., Wobus U., Steup M. Potato (Solanum tuberosum L. var. Desiree) were grown under controlled conditions (16-h light period at 130 to 200 micro-mol photons meter squared per second and 23�C; 8-h dark period at 18�C; 50% relative humidity). Leaf and tuber extracts were used in this study. In some cases chemical cross linking was carried out. Figure 4 Correspondence to M. Steup, Institut f�r Biochemie und Molekulare, Physiologie, Universit�t Potsdam, Maulbeerallee 2, D-14469 Potsdam, Germany Eur. J. Biochem. (0014-2956) 1998 Only protein-protein interactions imex curation albrecht-1998-1 Homodimers and heterodimers of Pho1-type phosphorylase isoforms in Solanum tuberosum L. as revealed by sequence-specific antibodies. soltu Solanum tuberosum (Potato) Potato anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Albrecht T., Greve B., Pusch K., Kossmann J., Buchner P., Wobus U., Steup M. Potato (Solanum tuberosum L. var. Desiree) were grown under controlled conditions (16-h light period at 130 to 200 micro-mol photons meter squared per second and 23�C; 8-h dark period at 18�C; 50% relative humidity). Leaf and tuber extracts were used in this study. In some cases chemical cross linking was carried out. Figure 4 Correspondence to M. Steup, Institut f�r Biochemie und Molekulare, Physiologie, Universit�t Potsdam, Maulbeerallee 2, D-14469 Potsdam, Germany Eur. J. Biochem. (0014-2956) 1998 Only protein-protein interactions imex curation phsl1_soltu Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic Starch phosphorylase L-1 protein protein soltu Solanum tuberosum (Potato) Potato MATANGAHLFNHYSSNSRFIHFTSRNTSSKLFLTKTSHFRRPKRCFHVNNTLSEKIHHPITEQGGESDLSSFAPDAASITSSIKYHAEFTPVFSPERFELPKAFFATAQSVRDSLLINWNATYDIYEKLNMKQAYYLSMEFLQGRALLNAIGNLELTGAFAEALKNLGHNLENVASQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVAEDWLEIGSPWEVVRNDVSYPIKFYGKVSTGSDGKRYWIGGEDIKAVAYDVPIPGYKTRTTISLRLWSTQVPSADFDLSAFNAGEHTKACEAQANAEKICYILYPGDESEEGKILRLKQQYTLCSASLQDIISRFERRSGDRIKWEEFPEKVAVQMNDTHPTLCIPELMRILIDLKGLNWNEAWNITQRTVAYTNHTVLPEALEKWSYELMQKLLPRHVEIIEAIDEELVHEIVLKYGSMDLNKLEEKLTTMRILENFDLPSSVAELFIKPEISVDDDTETVEVHDKVEASDKVVTNDEDDTGKKTSVKIEAAAEKDIDKKTPVSPEPAVIPPKKVRMANLCVVGGHAVNGVAEIHSEIVKEEVFNDFYELWPEKFQNKTNGVTPRRWIRFCNPPLSAIITKWTGTEDWVLKTEKLAELQKFADNEDLQNEWREAKRSNKIKVVSFLKEKTGYSVVPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMTAAERKTNFVPRVCIFGGKAFATYVQAKRIVKFITDVGATINHDPEIGDLLKVVFVPDYNVSVAELLIPASDLSEHISTAGMEASGTSNMKFAMNGCIQIGTLDGANVEIREEVGEENFFLFGAQAHEIAGLRKERADGKFVPDERFEEVKEFVRSGAFGSYNYDDLIGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRDQKRWTTMSILNTAGSYKFSSDRTIHEYAKDIWNIEAVEIA phsl2_soltu Alpha-1,4 glucan phosphorylase L-2 isozyme, chloroplastic/amyloplastic Starch phosphorylase L-2 STP-1 protein protein soltu Solanum tuberosum (Potato) Potato MATFAVSGLNSISSISSFNNNFRSKNSNILLSRRRILLFSFRRRRRSFSVSSVASDQKQKTKDSSSDEGFTLDVFQPDSTSVLSSIKYHAEFTPSFSPEKFELPKAYYATAESVRDTLIINWNATYEFYEKMNVKQAYYLSMEFLQGRALLNAIGNLGLTGPYADALTKLGYSLEDVARQEPDAALGNGGLGRLASCFLDSMATLNYPAWGYGLRYQYGLFKQLITKDGQEEVAENWLEMGNPWEIVRNDISYPVKFYGKVIEGADGRKEWAGGEDITAVAYDVPIPGYKTKTTINLRLWTTKLAAEAFDLYAFNNGDHAKAYEAQKKAEKICYVLYPGDESLEGKTLRLKQQYTLCSASLQDIIARFEKRSGNAVNWDQFPEKVAVQMNDTHPTLCIPELLRILMDVKGLSWKQAWEITQRTVAYTNHTVLPEALEKWSFTLLGELLPRHVEIIAMIDEELLHTILAEYGTEDLDLLQEKLNQMRILDNVEIPSSVLELLIKAEESAADVEKAADEEQEEEGKDDSKDEETEAVKAETTNEEEETEVKKVEVEDSQAKIKRIFGPHPNKPQVVHMANLCVVSGHAVNGVAEIHSEIVKDEVFNEFYKLWPEKFQNKTNGVTPRRWLSFCNPELSEIITKWTGSDDWLVNTEKLAELRKFADNEELQSEWRKAKGNNKMKIVSLIKEKTGYVVSPDAMFDVQIKRIHEYKRQLLNIFGIVYRYKKMKEMSPEERKEKFVPRVCIFGGKAFATYVQAKRIVKFITDVGETVNHDPEIGDLLKVVFVPDYNVSVAEVLIPGSELSQHISTAGMEASGTSNMKFSMNGCLLIGTLDGANVEIREEVGEDNFFLFGAQAHEIAGLRKERAEGKFVPDPRFEEVKAFIRTGVFGTYNYEELMGSLEGNEGYGRADYFLVGKDFPDYIECQDKVDEAYRDQKKWTKMSILNTAGSFKFSSDRTIHQYARDIWRIEPVELP p04045-p04045-1 Immunoretardation assay reveals homodimer of starch phosphorylase isoform in Ecoli 297196 N/A 297198 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) N/A 297198 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false p04045-stp1-1 Immunoretardation assay reveals heterodimer of starch phosphorylase isoforms in Ecoli 297196 N/A 297198 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) N/A 297199 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false stp_1-stp_1 Immunoretardation assay reveals homodimer of starch phosphorylase isoform in Ecoli 297196 N/A 297199 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) N/A 297199 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) physical association physical association false false false p04045-stp1 Coimmunoprecipitation assay reveals heterodimer of starch phosphorylase isoforms in potato leaf 297197 N/A 297199 unspecified role unspecified role prey prey N/A 297198 unspecified role unspecified role bait bait physical association physical association false false false This heterodimer was detected in potato leaf extracts only. p04045-p04045 Coimmunoprecipitation assay reveals homodimer of starch phosphorylase isoform in potato tubers and leaves 297197 N/A 297198 unspecified role unspecified role prey prey N/A 297198 unspecified role unspecified role bait bait physical association physical association false false false This homodimer was detected in potato leaves and tuber extracts