IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yoon-1997-3 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yoon DW., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung JU. fig 2a human lymphocyte library. Immunity (1074-7613) 1997 yoon-1997-1 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yoon DW., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung JU. fig 2a human lymphocyte library. Immunity (1074-7613) 1997 yoon-1997-2 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yoon DW., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung JU. fig 2a human lymphocyte library. Immunity (1074-7613) 1997 yoon-1997-4 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. human-jurkat Homo sapiens jurkat Human T cell leukemia anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Yoon DW., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung JU. Immmuprecipitate was incubated with gamma 32P ATP and analyzed by SDS-PAGE. fig 2a human lymphocyte library. Immunity (1074-7613) 1997 yoon-1997-5 Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. human-jurkat Homo sapiens jurkat Human T cell leukemia anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yoon DW., Lee H., Seol W., DeMaria M., Rosenzweig M., Jung JU. fig 2a human lymphocyte library. Immunity (1074-7613) 1997 tip_shv2c Tyrosine-protein kinase-interacting protein protein protein shv2c Saimiriine herpesvirus 2 (strain 488) (SaHV-2) Herpesvirus saimiri MANEGEEIELTEFPETEKERKDEEKLSSCSEETTNTSSSSGSDHVPVPIEVNVIIQNSSRTEDELQNSKEIELTGFQGKLSSCSEETTAPSSSYSSKQASVFIEENGDNETSTYRPQNVLTNLNSLYTTFEDARAQGKGMVRHKSEDLQSFLEKYPPDFRKPKRDLSATWDPGMPTPPLPPRPANLGERQASTVRLHVKESNCKQPRERKANERNIVKDLKRLENKINVIICLVVVILAVLLLVTVLSILHIGMKS nxf1_human Nuclear RNA export factor 1 NXF1 TAP Tip-associating protein Tip-associated protein mRNA export factor TAP protein protein human Homo sapiens Human MADEGKSYSEHDDERVNFPQRKKKGRGPFRWKYGEGNRRSGRGGSGIRSSRLEEDDGDVAMSDAQDGPRVRYNPYTTRPNRRGDTWHDRDRIHVTVRRDRAPPERGGAGTSQDGTSKNWFKITIPYGRKYDKAWLLSMIQSKCSVPFTPIEFHYENTRAQFFVEDASTASALKAVNYKILDRENRRISIIINSSAPPHTILNELKPEQVEQLKLIMSKRYDGSQQALDLKGLRSDPDLVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILNLSGNELKSERELDKIKGLKLEELWLDGNSLCDTFRDQSTYISAIRERFPKLLRLDGHELPPPIAFDVEAPTTLPPCKGSYFGTENLKSLVLHFLQQYYAIYDSGDRQGLLDAYHDGACCSLSIPFIPQNPARSSLAEYFKDSRNVKKLKDPTLRFRLLKHTRLNVVAFLNELPKTQHDVNSFVVDISAQTSTLLCFSVNGVFKEVDGKSRDSLRAFTRTFIAVPASNSGLCIVNDELFVRNASSEEIQRAFAMPAPTPSSSPVPTLSPEQQEMLQAFSTQSGMNLEWSQKCLQDNNWDYTRSAQAFTHLKAKGEIPEVAFMK lck_human Tyrosine-protein kinase Lck Protein YT16 Proto-oncogene Lck T cell-specific protein-tyrosine kinase LCK Lymphocyte cell-specific protein-tyrosine kinase p56-LCK Leukocyte C-terminal Src kinase protein protein human Homo sapiens Human MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP tip-tap-3 in vivo Co-IP: bait= herpes virus saimiri Tip, prey= tap 332596 N/A 332601 unspecified role unspecified role bait bait au1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 332602 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The start methionine of human TAP protein in the article corresponds the methionine 61 of the UniProtKB entry Q9UBU9. tip-tap-4 in vivo Co-IP: bait= tap, prey= herpes virus saimiri Tip 332596 N/A 332601 unspecified role unspecified role prey prey au1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 332602 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false The start methionine of Human TAP protein in the article corresponds the methionine 61 of the UniProtKB entry Q9UBU9. tip-tap-1 Y2H: bait= herpes virus saimiri Tip, prey= tap 332597 N/A 332601 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 332602 unspecified role unspecified role prey prey n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 332620 332618 physical association physical association false false false tip-tap-2 in vitro pull-down: bait= herpes virus saimiri Tip, prey= tap 332598 N/A 332602 unspecified role unspecified role bait bait n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 332601 unspecified role unspecified role prey prey c-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 physical association physical association false false false Cos-1 cell lysate expressing the viral protein tip was applied on the pull-down column. tip-tap-6 in vivo Co-IP: bait= herpes virus saimiri Tip, phosphorylated preys= tap, Lck 332599 N/A 332603 unspecified role unspecified role prey prey region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 332601 unspecified role unspecified role bait bait au1 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 332602 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false association association false false false The start methionine of human TAP protein in the article corresponds the methionine 61 of the UniProtKB entry Q9UBU9. A 50 kDa phosphorylated protein was also detected. tip-tap-7 in vivo Co-IP: bait= tap, prey= herpes virus saimiri Tip 332600 N/A 332602 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 332601 unspecified role unspecified role prey prey au1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false The start methionine of human TAP protein in the article corresponds the methionine 61 of the UniProtKB entry Q9UBU9.